Key Product Details

Species Reactivity

MERS-CoV

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1038729
View all Nucleocapsid Primary Antibodies »

Product Specifications

Immunogen

Spodoptera frugiperda insect ovarian cell line Sf21-derived mouse MERS-CoV Nucleocapsid
Met1-Thr411
Accession # YP_007188586.1

Specificity

Detects Mouse MERS-CoV Nucleocapsid in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for MERS-CoV Nucleocapsid Antibody

Detection of MERS-CoV MERS-CoV Nucleocapsid by Western Blot.

Western blot shows recombinant MERS-CoV Nucleocapsid protein. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Mouse MERS-CoV Nucleocapsid Monoclonal Antibody (Catalog # MAB10729) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for MERS-CoV Nucleocapsid at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Nucleocapsid by Simple WesternTM.

Simple Western lane view shows recombinant MERS-CoV nucleocapsid, loaded at 0.2 mg/mL. A specific band was detected for Nucleocapsid at approximately 57 kDa (as indicated) using 25 µg/mL of Mouse Anti-MERS-CoV Nucleocapsid Monoclonal Antibody (Catalog # MAB10729). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for MERS-CoV Nucleocapsid Antibody

Application
Recommended Usage

Simple Western

25 µg/mL
Sample: Recombinant MERS-CoV nucleocapsid

Western Blot

1 µg/mL
Sample: Recombinant MERS-CoV Nucleocapsid protein

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Nucleocapsid

MERS-CoV, which causes the Middles East Respiratory Syndrome (MERS), belongs to a family of viruses known as coronaviruses that are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1). While the S, E and M proteins build up the viral envelope, the N protein is involved transcription, replication and packaging of the viral RNA genome into a helical ribonucleocapsid (RNP) (1, 2). The MERS-CoV N protein is a ~45 kDa protein composed of two independent structural domains connected by a linker region. The N-terminal region contains an Intrinsically Disordered Region (3) and an RNA binding domain (4), the linker region interacts with the M protein and the C-terminal region contains a self-association domain (1, 2). The MERS-CoV N protein shares 46.3% and 4.5% amino acid sequence identity with SARS-CoV-1 and SARS-CoV-2 N protein, respectively. MERS-CoV N proteins have been shown to inhibit Type I Interferon(IFN) production(1). In addition, the N protein is an abundant protein during coronavirus infection and displays high immunogenic activity, making it a promising therapeutic target (5-7).

References

  1. Li, Y. et al. (2019) Engineering. 5:940.
  2. Hurst, K. R. et al. (2009) J. Virol. 83:7221.
  3. Wang, Y. et al. (2015) Acta. Crystallogr. F. Struct. Biol. Commun. 71:977.
  4. Papageorgiou, N. et al. (2016) Acta. Crystallogr. D. Struct. Biol. 72:192.
  5. Che, X. Y. et al. (2004) J. Clin. Microbiol. 42:2629.
  6. Guan, M. et al. (2004) Clin. Diagn. Lab. Immunol. 11:287.
  7. Chang, C-K. et al.(2016) Drug Discov. Today. 21:562.

Long Name

Nucleocapsid Protein

Alternate Names

N Protein

Entrez Gene IDs

918763 (HCoV-229E); 3200423 (HCoV-HKU1); 2943504 (HCoV-NL63); 39105221 (HCoV-OC43); 14254601 (MERS-CoV); 1489678 (SARS-CoV); 43740575 (SARS-CoV-2)

Gene Symbol

N

UniProt

YP_007188586.1

Additional Nucleocapsid Products

Product Documents for MERS-CoV Nucleocapsid Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for MERS-CoV Nucleocapsid Antibody

For research use only

Related Research Areas

Infectious Diseases

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