Mouse Activin RIB/ALK-4 Antibody

Catalog # Availability Size / Price Qty
AF1477
AF1477-SP
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Mouse Activin RIB/ALK-4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Activin RIB/ALK‑4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross‑reactivity with recombinant human (rh) Activin RIB is observed, 5% cross‑reactivity with rhActivin R1A is observed and less than 1% cross‑reactivity with rhActivin RIIA and rhActivin RIIB is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Activin RIB/ALK‑4
Leu32-Glu126
Accession # Q61271
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Activin RIB/ALK‑4 Fc Chimera (Catalog # 1477-AR)
Immunohistochemistry
5-15 µg/mL
Immersion fixed frozen sections of mouse embryo (E13-15)
Blockade of Receptor-ligand Interaction
In a functional ELISA, 1-4 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Mouse Cripto (Catalog # 1538‑CR) to immobilized Recombinant Mouse Activin RIB/ALK-4 Fc Chimera (Catalog # 1477‑AR) coated at 1 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Activin RIB/ALK-4

Activin RIB, also known as ALK-4, is a glycosylated 58 kDa type I transmembrane receptor that belongs to the superfamily of TGF-beta serine/threonine kinase receptors. Activin RIB associates with Activin RIIB to form a receptor complex for activin and inhibin molecules (1). These ligands bind to Activin RIIB which then associates with, and phosphorylates, the cytoplasmic domain of Activin RIB to initiate signal transduction (2, 3). Mature mouse Activin RIB consists of a 103 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 356 aa cytoplasmic region that includes the kinase domain (4). Within the ECD, mouse Activin RIB shares 93% and 98% aa sequence identity with human and rat Activin RIB, respectively. It shares 23%‑38% aa sequence identity with other mouse type I receptors Activin RIA, BMPR-IA, BMPR-IB, and TGF-beta RI. Activin receptor signaling is modulated by the direct interaction of Activin RIB with cripto or inhibin binding protein (5‑7). Activin RIB is excluded from the signaling complex if Activin RIIB first binds inhibin and betaglycan (8). Activin RIB functions in a wide variety of growth and differentiation processes, including embryonic cell fate and axis determination, cell proliferation, apoptosis, and tumorigenesis (1, 9, 10).

References
  1. Chen, Y.-G. et al. (2006) Exp. Biol. Med. 231:534.
  2. Attisano, L. et al. (1996) Mol. Cell. Biol. 16:1066.
  3. Tsuchida, K. et al. (1995) Endocrinology 136:5493.
  4. Ebner, R. et al. (1993) Science 260:1344.
  5. Chapman, S.C. and T.K. Woodruff (2001) Mol. Endocrinol. 15:668.
  6. Bianco, C. et al. (2002) Mol. Cell. Biol. 22:2586.
  7. Gray, P.C. et al. (2003) Proc. Natl. Acad. Sci. 100:5193.
  8. Lewis, K.A. et al. (2000) Nature 404:411.
  9. Gu, Z. et al. (1998) Genes Dev. 12:844.
  10. Chen, Y. et al. (2004) Dev. Biol. 268:280.
Long Name
Activin Receptor IB
Entrez Gene IDs
91 (Human); 11479 (Mouse)
Alternate Names
activin A receptor, type IB; Activin receptor type IB; Activin receptor-like kinase 4; Activin RIB; ActivinRIB; ACTRIB; ACVR1B; ACVRLK4ACTR-IB; ALK4; ALK-4; ALK4activin A receptor, type II-like kinase 4; EC 2.7.11; EC 2.7.11.30; Serine/threonine-protein kinase receptor R2; SKR2activin receptor type-1B

Product Datasheets

Citation for Mouse Activin RIB/ALK-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Postnatal regulation of germ cells by activin: the establishment of the initial follicle pool.
    Authors: Bristol-Gould SK, Kreeger PK, Selkirk CG, Kilen SM, Cook RW, Kipp JL, Shea LD, Mayo KE, Woodruff TK
    Dev. Biol., 2006;298(1):132-48.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Mouse Activin RIB/ALK-4 Antibody

There are currently no reviews for this product. Be the first to review Mouse Activin RIB/ALK-4 Antibody and earn rewards!

Have you used Mouse Activin RIB/ALK-4 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review