Mouse Angiopoietin-3 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Angiopoietin-3 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Angiopoietin‑2 or rhAngoipoietin-3 was observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse Angiopoietin-3
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Angiopoietin‑3 (Catalog # 738-AN)
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Angiopoietin‑3 in Rat Kidney. Angiopoietin‑3 was detected in perfusion fixed frozen sections of rat kidney using 5 µg/mL Goat Anti-Mouse Angiopoietin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF738) overnight at
4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Angiopoietin-3

Mouse angiopoietin-3 (Ang-3) (1), is a secreted glycoprotein belonging to the angiopoietin family. It has the characteristic structural motifs of angiopoietins including the coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. Mouse Ang-3 cDNA encodes a 509 amino acid (aa) precursor protein with a 21 aa signal peptide. It shares 47%, 46%, and 54% aa sequence identity with mouse Ang-1, mouse Ang-2, and human Ang-4, respectively. Although the sequence homology is much higher between the human and mouse counterparts for Ang-1 (97%) and Ang-2 (85%), mouse Ang-3 is believed to be an ortholog of human Ang-4 based on chromosomal localization studies (1, 2). Human Ang-4 is highly expressed in lung and in cultured human umbilical vein endothelial cells (HUVECs). In contrast, mouse Ang-3 is expressed in multiple mouse tissues. Human ANG-4 is an agonist that can bind and activate Tie-2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells (2, 3). Mouse Ang-3 has been reported to be a Tie-2 antagonist. It is likely that mouse Ang-3, like Ang-2, may exert agonist or antagonist activities depending on the cell context (1, 3, 4).

  • References:
    1. Valenzuela, D.M. et al. (1999) Proc. Natl. Acad. Sci. USA 96:1904.
    2. Nishimura, M. et al. (1999) FEBS Lett. 448:254.
    3. Jones, N. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:257.
    4. Teichert-Kuliszewska, K. et al. (2001) Cardiovasc. Res. 49:659.
  • Entrez Gene IDs:
    11602 (Mouse)
  • Alternate Names:
    Angiopoietin-3; ANGPT3
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