Mouse Carbonic Anhydrase IV/CA4 Antibody

(10 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Carbonic Anhydrase IV/CA4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross‑reactivity with recombinant human (rh) CA4 is observed and less than 2% cross-reactivity with rhCA3, rhCA10, rhCA13, recombinant mouse (rm) CA9, and rmCA12 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase IV/CA4
    Glu18-Ser277
    Accession # Q64444
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.25 µg/mL
    See below
  • Simple Western
    12.5 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Mouse Carbonic Anhydrase IV (Catalog # 2414-CA), see our available Western blot detection antibodies
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse Carbonic Anhydrase IV/CA4 by Western Blot. Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Carbonic Anhydrase IV/CA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2414) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Carbonic Anhydrase IV/CA4 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Carbonic Anhydrase IV/CA4 in Mouse Kidney. Carbonic Anhydrase IV/CA4 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Carbonic Anhydrase IV/CA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2414) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse Carbonic Anhydrase IV/CA4 by Simple WesternTM. Simple Western lane view shows lysates of mouse lung tissue and mouse kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase IV/CA4 at approximately 51-52 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Mouse Carbonic Anhydrase IV/CA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2414) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase IV/CA4

Carbonic anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. The deduced amino acid sequence of mouse CA4 consists of a signal peptide (residues 1‑17), an ectodomain (residues 18‑277) and a pro peptide (residues 278‑305), which is removed in the mature form (2). The native enzyme is attached to the membrane by a GPI‑anchor. Recombinant mouse CA4 corresponds to the ectodomain and exhibits the activity as described in Activity Assay Protocol.

  • References:
    1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
    2. Tamai, S. et al. (1996) Biochem. Genet. 34:31.
  • Entrez Gene IDs:
    762 (Human); 12351 (Mouse)
  • Alternate Names:
    CA4; CAIV; CA-IV; Car4; Carbonate dehydratase IV; carbonic anhydrase 4; Carbonic Anhydrase IV; carbonic anhydrase IVRP17; carbonic dehydratase IV; EC 4.2.1.1; retinitis pigmentosa 17 (autosomal dominant); RP17
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. The cellular mechanism for water detection in the mammalian taste system
    Authors: D Zocchi, G Wennemuth, Y Oka
    Nat. Neurosci., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Transcriptome analyses of taste organoids reveal multiple pathways involved in taste cell generation
    Authors: W Ren, E Aihara, W Lei, N Gheewala, H Uchiyama, RF Margolskee, K Iwatsuki, P Jiang
    Sci Rep, 2017;7(1):4004.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  3. Bcl11b/Ctip2 is required for development of lingual papillae in mice
    Authors: Yugo Nishiguchi
    Dev Biol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  4. Normal Fertility Requires the Expression of Carbonic Anhydrases II and IV in Sperm.
    Authors: Wandernoth P, Mannowetz N, Szczyrba J, Grannemann L, Wolf A, Becker H, Sly W, Wennemuth G
    J Biol Chem, 2015;290(49):29202-16.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  5. Induction of ectopic taste buds by SHH reveals the competency and plasticity of adult lingual epithelium.
    Authors: Castillo D, Seidel K, Salcedo E, Ahn C, de Sauvage F, Klein O, Barlow L
    Development, 2014;141(15):2993-3002.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC- Frozen
  6. Carbonic anhydrase IV is expressed on IL-5-activated murine eosinophils.
    Authors: Wen T, Mingler M, Wahl B, Khorki M, Pabst O, Zimmermann N, Rothenberg M
    J Immunol, 2014;192(12):5481-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  7. Expression patterns and subcellular localization of carbonic anhydrases are developmentally regulated during tooth formation.
    Authors: Reibring C, El Shahawy M, Hallberg K, Kannius-Janson M, Nilsson J, Parkkila S, Sly W, Waheed A, Linde A, Gritli-Linde A
    PLoS ONE, 2014;9(5):e96007.
  8. Defects in the peripheral taste structure and function in the MRL/lpr mouse model of autoimmune disease.
    Authors: Kim A, Feng P, Ohkuri T, Sauers D, Cohn ZJ, Chai J, Nelson T, Bachmanov AA, Huang L, Wang H
    PLoS ONE, 2012;7(4):e35588.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  9. CO2 chemosensing in rat oesophagus.
    Authors: Akiba Y, Mizumori M, Kuo M, Ham M, Guth PH, Engel E, Kaunitz JD
    Gut, 2008;57(12):1654-64.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  10. Carbonic anhydrases and mucosal vanilloid receptors help mediate the hyperemic response to luminal CO2 in rat duodenum.
    Authors: Akiba Y, Ghayouri S, Takeuchi T, Mizumori M, Guth PH, Engel E, Swenson ER, Kaunitz JD
    Gastroenterology, 2006;131(1):142-52.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
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