Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Cathepsin E Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin E
Gln19-Pro397
Accession # P70269

Specificity

Detects both pro and mature mouse Cathepsin E in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human Cathepsin E and less than 1% cross-reactivity with recombinant mouse Cathepsin D is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Cathepsin E Antibody

Cathepsin E antibody in Mouse Lung by Immunohistochemistry (IHC-Fr).

Cathepsin E in Mouse Lung.

Cathepsin E was detected in perfusion fixed frozen sections of mouse lung using Goat Anti-Mouse Cathepsin E Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1130) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of type II alveolar cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse Cathepsin E Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse lung

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Cathepsin E (Catalog # 1130-AS), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Cathepsin E (Catalog # 1130-AS)

Reviewed Applications

Read 1 review rated 5 using AF1130 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cathepsin E

Cathepsin E is an intracellular aspartic protease of the pepsin family (1, 2). Unlike Cathepsin D, another member of the same family and a lysosomal protease with relatively ubiquitous distribution, Cathepsin E is not a lysosomal enzyme and has a limited cell and tissue distribution. However, both Cathepsins D and E play an important role in the degradation of proteins, the generation of bioactive proteins, and antigen processing (3). Both enzymes are efficient in cleaving the Swedish mutant of amyloid precursor protein (APP) at the beta site but show almost no reactivity with the wild-type APP (4). Mouse Cathepsin E is synthesized as a precursor protein, consisting of a signal peptide (residues 1-18), a propeptide (residues 19-59), and a mature chain (residues 60-397) (1).

References

  1. Tatnell, P.J. et al. (1997) FEBS Lett. 408:62.
  2. Kay, J. and P.J. Tatnell (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al., eds.), p. 33,  Academic Press, San Diego.
  3. Tsukuba, T. et al. (2000) Mol. Cells 10:601.
  4. Gruninger-Leitch, F. et al. (2000) Nat. Biotechnol. 18:66.

Alternate Names

CTSE

Entrez Gene IDs

1510 (Human); 13034 (Mouse)

Gene Symbol

CTSE

UniProt

P70269

Additional Cathepsin E Products

Product Documents for Mouse Cathepsin E Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Cathepsin E Antibody

For research use only

Citations for Mouse Cathepsin E Antibody

Customer Reviews for Mouse Cathepsin E Antibody (1)

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  • Mouse Cathepsin E Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Liver tissue, Lung tissue and Spleen tissue
    Species: Human and Mouse
    Verified Customer | Posted 11/01/2016
    Mouse Cathepsin E Antibody AF1130

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Protocols

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