Mouse CCL25/TECK Antibody

(5 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCL25/TECK in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human TECK is observed. 
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse CCL25/TECK
    Gln24-Asn144
    Accession # O35903
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse CCL25/TECK (Catalog # 481-TK)
  • Immunohistochemistry
    5-15 µg/mL
    Perfusion fixed frozen sections of mouse intestine
  • Neutralization
    Measured by its ability to neutralize CCL25/TECK-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR9. 55 μg/mL of this antibody will neutralize 1.6 μg/mL of recombinant mouse TECK‑induced chemotactic effect on BaF/3 hCCR9 cells by approximately 80‑90%.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CCL25/TECK and Neutral­ization by Mouse CCL25/
TECK Antibody.
Recombinant Mouse CCL25/TECK (Catalog # 481‑TK) chemo­attracts the BaF3 mouse pro‑B cell line transfected with human CCR9 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Mouse CCL25/TECK (1.6 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL25/TECK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑481‑NA). 55 μg/mL of this antibody will neutralize rmTECK-induced chemotactic effect on BaF/3 hCCR9 cells by approximately 80‑90%.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL25/TECK

CCL25 (thymus-expressed chemokine) is a novel CC chemokine that is distantly related (twenty-some % amino acid sequence identity) to other CC chemokines. Mouse CCL25 cDNA encodes a 144 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 121 residue mature protein. Human CCL25 cDNA has also been cloned and shown to encode a 151 amino acid protein that exhibits 49% amino acid sequence identity to mouse CCL25. The expresssion of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. The gene for mouse CCL25 has been mapped to chromosome 8 rather than chromosome 11 where many mouse CC chemokines are clustered. Recombinant mouse CCL25 has been shown to be chemotactic for activated macrophages, dendritic cells and thymocytes.

  • References:
    1. Vicari, A.P. et al. (1997) Immunity 7:291.
  • Entrez Gene IDs:
    6370 (Human); 20300 (Mouse)
  • Alternate Names:
    C-C motif chemokine 25; CCL25; chemokine (C-C motif) ligand 25; Chemokine TECK; Ck beta-15; Ckb15; SCYA25TECKvar; small inducible cytokine subfamily A (Cys-Cys), member 25; Small-inducible cytokine A25; TECK; TECKMGC150327; thymus expressed chemokine; Thymus-expressed chemokine
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Comprehensive models of human primary and metastatic colorectal tumors in immunodeficient and immunocompetent mice by chemokine targeting.
    Authors: Chen H, Sun J, Huang Z, Hou H, Arcilla M, Rakhilin N, Joe D, Choi J, Gadamsetty P, Milsom J, Nandakumar G, Longman R, Zhou X, Edwards R, Chen J, Chen K, Bu P, Wang L, Xu Y, Munroe R, Abratte C, Miller A, Gumus Z, Shuler M, Nishimura N, Edelmann W, Shen X, Lipkin S
    Nat Biotechnol, 2015;33(6):656-60.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  2. Chemokine 25-induced signaling suppresses colon cancer invasion and metastasis.
    J. Clin. Invest., 2012;122(9):3184-96.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  3. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010;120(3):907-23.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. The role of CCL21 in recruitment of T-precursor cells to fetal thymi.
    Authors: Ueno T, Kuse S, Saito F, Nitta T, Kakiuchi T, Hollander GA, Takahama Y
    Blood, 2005;105(1):31-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  5. CC chemokine ligands 25 and 28 play essential roles in intestinal extravasation of IgA antibody-secreting cells.
    Authors: Hieshima K, Kawasaki Y, Hanamoto H, Nakayama T, Nagakubo D, Kanamaru A, Yoshie O
    J. Immunol., 2004;173(6):3668-75.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
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