Mouse CD3 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB4841R-100
MAB4841R-SP
Detection of CD3 in Mouse Splenocytes by Flow Cytometry.
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Mouse CD3 Antibody Summary

Species Reactivity
Mouse
Specificity
Reacts with mouse TCR-associated CD3 complex that occurs on thymocytes and mature T cells.
Source
Recombinant Monoclonal Rat IgG Clone # 17A2R
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
T cell hybridoma D1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
5-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of CD3 in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of CD3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with either Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB8481R, filled histogram) or Rat IgG2B Isotype Control (Catalog # MAB006, open histogram) followed by PE-conjugated anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry CD3 in  Mouse Splenocytes. View Larger

CD3 in Mouse Splenocytes. CD3 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841R) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry CD3 in  Mouse Spleen Tissue. View Larger

CD3 in Mouse Spleen Tissue. CD3 was detected in immersion fixed paraffin-embedded sections of mouse spleen tissue using Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841R) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD3

Monoclonal 17A2 was generated by immunizing rats with a T-cell hybridoma (1). This antibody has been shown to react with the epsilon chain of the CD3 complex (1). The CD3 protein complex is expressed on thymocytes and mature T-cells and, therefore, is a useful reagent to monitor T-cell frequencies in tissues.

References
  1. Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.
Entrez Gene IDs
916 (Human); 12503 (Mouse)
Alternate Names
CD3 antigen, delta subunit; CD3; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3-epsilon; CD3g antigen; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3G; CD3-GAMMA; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; MGC138597; T3DOKT3, delta chain; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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