Mouse CD68/SR-D1 Antibody Summary
Accession # P31996
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CD68 in Raw264.7 Mouse Cell line by Flow Cytometry. Raw264.7 mouse cell line was stained with Rabbit Anti-Mouse CD68 Monoclonal Antibody (Catalog # MAB101141, filled histogram) or Normal Rabbit IgG Control (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (1x) (Catalog # FC004) and permeabilized with ice-cold methanol.
CD68/SR‑D1 in Mouse Liver. CD68/SR‑D1 was detected in immersion fixed paraffin-embedded sections of mouse liver using Rabbit Anti-Mouse CD68/SR‑D1 Monoclonal Antibody (Catalog # MAB101141) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Macrosialin (CD68) is a macrophage-restricted glycosylated transmembrane protein. It belongs to the lamp family and carries out specialized functions in dedicated phagocytic cells. Macrosialin binds to tissue- and organ-specific lectins to allow homing of macrophages to specific targets. Its presence in macrophages is useful in diagnosing conditions related to proliferation or abnormality of these cells.
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