Mouse CD68/SR-D1 Antibody Summary
Asp21-Pro282
Accession # P31996
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CD68 in Mouse Liver via seqIF™ staining on COMET™ CD68 was detected in immersion fixed paraffin-embedded sections of mouse Liver using Rabbit Anti-Mouse CD68, Monoclonal Antibody (Catalog #MAB101141) at 5ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm of Kupffer cells. Protocol available in COMET™ Panel Builder.
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Detection of CD68 in Mouse Brain Medulla via seqIF™ staining on COMET™ CD68 was detected in perfusion fixed-frozen sections of mouse Brain Medulla using Rabbit Anti-Mouse CD68, Monoclonal Antibody (Catalog#MAB101141) at 20ug/mL at 27° Celsius for 8minutes. Before incubation with the primary antibody, tissue underwent preprocessing by incubating tissue with Multi Staining Buffer (Lunaphore Catalog # BU06) for 5 minutes at room temperature followed by a 20-minute incubation in Tris-Buffered Saline + 0.2% Triton at room temperature. Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.
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CD68/SR‑D1 in Mouse Liver. CD68/SR-D1 was detected in immersion fixed paraffin-embedded sections of mouse liver using Rabbit Anti-Mouse CD68/SR-D1 Monoclonal Antibody (Catalog # MAB101141) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Detection of CD68 in Raw264.7 Mouse Cell line by Flow Cytometry. Raw264.7 mouse cell line was stained with Rabbit Anti-Mouse CD68 Monoclonal Antibody (Catalog # MAB101141, filled histogram) or Normal Rabbit IgG Control (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (1x) (Catalog # FC004) and permeabilized with ice-cold methanol.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD68/SR-D1
Macrosialin (CD68) is a macrophage-restricted glycosylated transmembrane protein. It belongs to the lamp family and carries out specialized functions in dedicated phagocytic cells. Macrosialin binds to tissue- and organ-specific lectins to allow homing of macrophages to specific targets. Its presence in macrophages is useful in diagnosing conditions related to proliferation or abnormality of these cells.
Product Datasheets
Citation for Mouse CD68/SR-D1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Transcriptomic signatures of cold acclimated adipocytes reveal CXCL12 as a Brown autocrine and paracrine chemokine
Authors: Agueda-Oyarzabal, M;Isidor, MS;Pluci?ska, K;Ingerslev, LR;Dmytriyeva, O;Petersen, PSS;Laftih, S;Pontoppidan, AB;Henningsen, JB;Rupar, K;Brown, EL;Schwartz, TW;Barrès, R;Gerhart-Hines, Z;Schéele, CC;Emanuelli, B;
Molecular metabolism
Species: Mouse
Sample Types:
Applications: In Situ Hybridization
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Deparaffin; Citrate acid (6.0) 30min while steaming; 1:100. Works very well! :)
