Mouse CD68/SR-D1 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
Detection  of CD68 in Raw264.7 Mouse Cell line by Flow Cytometry.
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Product Details
Supplemental Products

Mouse CD68/SR-D1 Antibody Summary

Species Reactivity
Detects mouse CD68 in direct ELISAs.
Recombinant Monoclonal Rabbit IgG Clone # 2449D
Protein A or G purified from cell culture supernatant
Chinese Hamster Ovary cell line, CHO-derived mouse CD68
Accession # P31996
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
3-25 µg/mL
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Intracellular Staining by Flow Cytometry Detection  of CD68 in Raw264.7 Mouse Cell line by Flow Cytometry. View Larger

Detection of CD68 in Raw264.7 Mouse Cell line by Flow Cytometry. Raw264.7 mouse cell line was stained with Rabbit Anti-Mouse CD68 Monoclonal Antibody (Catalog # MAB101141, filled histogram) or Normal Rabbit IgG Control (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (1x) (Catalog # FC004) and permeabilized with ice-cold methanol.

Immunohistochemistry CD68/SR‑D1  in Mouse Liver. View Larger

CD68/SR‑D1 in Mouse Liver. CD68/SR‑D1 was detected in immersion fixed paraffin-embedded sections of mouse liver using Rabbit Anti-Mouse CD68/SR‑D1 Monoclonal Antibody (Catalog # MAB101141) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD68/SR-D1

Macrosialin (CD68) is a macrophage-restricted glycosylated transmembrane protein.  It belongs to the lamp family and carries out specialized functions in dedicated phagocytic cells.  Macrosialin binds to tissue- and organ-specific lectins to allow homing of macrophages to specific targets.  Its presence in macrophages is useful in diagnosing conditions related to proliferation or abnormality of these cells.

Entrez Gene IDs
968 (Human); 12514 (Mouse); 287435 (Rat)
Alternate Names
CD68 antigenmacrophage antigen CD68; CD68 molecule; CD68; DKFZp686M18236; gp110; Macrosialin; SCARD1; scavenger receptor class D, member 1; SRD1; SR-D1

Product Datasheets


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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Normal Rabbit IgG Control


Reconstitution Buffers

Secondary Antibodies

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