Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CD69 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD69
Gly64-Arg199
Accession # P37217

Specificity

Detects mouse CD69 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross‑reactivity with recombinant human CD69 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse CD69 Antibody

Detection of CD69 in mouse splenocytes treated with 50ng/mL PMA and 200ng/mL Ionomycin overnight vs unstimulated mouse splenocytes by Flow Cytometry

Mouse splenocytes treated with 50ng/mL PMA and 200ng/mL Ionomycin overnight (A) vs unstimulated mouse splenocytes (B) were stained with Goat Anti-Mouse CD69 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2386) and Rat Anti-Mouse CD3 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB4841F) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CD69 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: - CTLL‑2 mouse cytotoxic T cell line- Mouse splenocytes treated with 50ng/mL PMA and 200ng/mL Ionomycin overnight vs unstimulated mouse splenocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CD69

Reviewed Applications

Read 1 review rated 4 using AF2386 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD69

CD69, also known as Leu 23, AIM, EA-1 and MLR-3, is a type II transmembrane glycoprotein and is expressed on activated T cells, B cells, NK cells, neutrophils and eosinophils, Langerhans cells and platelets. The C-terminal extracellular domain of mouse CD69 shares approximately 57% amino acid sequence homology with the corresponding region of human CD69.

Alternate Names

CD69, EA-1, Leu23, MLR-3, p60

Entrez Gene IDs

969 (Human); 12515 (Mouse)

Gene Symbol

CD69

UniProt

P37217

Additional CD69 Products

Product Documents for Mouse CD69 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse CD69 Antibody

For research use only

Citations for Mouse CD69 Antibody

Customer Reviews for Mouse CD69 Antibody (1)

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Customer Images

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  • Mouse CD69 Antibody
    Name: Efstathios Stamatiades
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Mammary gland tissue
    Species: Mouse
    Verified Customer | Posted 07/09/2019
    Cryosections from PLP immersion fixed PyMT mammary gland tumors were stained for CD69 (1:100) for 4h @ RT. After washing, tissue was stained with Donkey anti-Goat alexa546 2ndary antibody for 1h at RT. CD3 in green, CD69 in red, nuclear staining (Hoechst) in blue.
    Mouse CD69 Antibody AF2386

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