Mouse Common  gamma  Chain/IL-2 R gamma Antibody

Catalog # Availability Size / Price Qty
MAB7842
MAB7842-SP
Mouse Common  gamma  Chain/IL-2 R gamma  Antibody in Data
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Mouse Common  gamma  Chain/IL-2 R gamma Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Common gamma Chain/IL-2 R gamma in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL-2 R alpha, rmIL‑2 R beta, recombinant human (rh) IL-2 R gamma, rhIL-15 R alpha, recombinant rat IL-2 R alpha, or recombinant canine IL-2 R alpha is observed.
Source
Monoclonal Rat IgG2B Clone # 644346
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf21-derived recombinant mouse Common gamma Chain/IL-2 R gamma
Trp23-Ala263 (predicted)
Accession # P34902
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Common ? Chain/IL-2 R? antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of Common gamma Chain/IL‑2 R gamma in Mouse Splenocytes by Flow Cytometry. CD3+mouse splenocytes were stained with Mouse Common gamma Chain/IL-2 R gamma Monoclonal Antibody (Catalog # MAB7842, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Fluorescein-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0104B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Common gamma Chain/IL-2 R gamma

The Common gamma Chain, also known as CD132, is a transmembrane protein belonging to the hematopoietin receptor family. gamma c is a signaling component of the functional receptor complexes for IL-2, IL-4, IL-7, IL-9, and IL-15.

Long Name
IL-2 Receptor gamma
Entrez Gene IDs
3561 (Human); 16186 (Mouse)
Alternate Names
CD132 antigen; CD132; CIDX; combined immunodeficiency, X-linked; common cytokine receptor gamma chain; Common gamma Chain; common gamma-chain; cytokine receptor common subunit gamma; gamma(c); IL-2 R gamma; IL-2 receptor subunit gamma; IL2R gamma; IL-2R subunit gamma; IL2RG; IL-2RG; IMD4; interleukin 2 receptor, gamma; Interleukin-2 receptor subunit gamma; P64; SCIDX; SCIDX1; severe combined immunodeficiency

Product Datasheets

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