Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 644346
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Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf21-derived recombinant mouse Common gamma Chain/IL-2 R gamma
Trp23-Ala263 (predicted)
Accession # P34902

Specificity

Detects mouse Common gamma Chain/IL-2 R gamma in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL-2 R alpha, rmIL‑2 R beta, recombinant human (rh) IL-2 R gamma, rhIL-15 R alpha, recombinant rat IL-2 R alpha, or recombinant canine IL-2 R alpha is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Common  gamma  Chain/IL‑2 R gamma Antibody

Detection of Common ? Chain/IL-2 R? antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of Common gamma Chain/IL‑2 R gamma in Mouse Splenocytes by Flow Cytometry.

CD3+mouse splenocytes were stained with Mouse Common gamma Chain/IL-2 R gamma Monoclonal Antibody (Catalog # MAB7842, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Fluorescein-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0104B).

Applications for Mouse Common  gamma  Chain/IL‑2 R gamma Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: CD3+ mouse splenocytes

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Common gamma Chain/IL-2 R gamma

The Common gamma Chain, also known as CD132, is a transmembrane protein belonging to the hematopoietin receptor family. gamma c is a signaling component of the functional receptor complexes for IL-2, IL-4, IL-7, IL-9, and IL-15.

Long Name

IL-2 Receptor gamma

Alternate Names

CD132, IL-2 R gamma, IL2R gamma, IL2RG

Entrez Gene IDs

3561 (Human); 16186 (Mouse)

Gene Symbol

IL2RG

UniProt

Additional Common gamma Chain/IL-2 R gamma Products

Product Documents for Mouse Common  gamma  Chain/IL‑2 R gamma Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Common  gamma  Chain/IL‑2 R gamma Antibody

For research use only

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