Mouse Common  gamma  Chain/IL-2 R gamma Antibody

Catalog # Availability Size / Price Qty
MAB7842
MAB7842-SP
Detection of Common  gamma  Chain/IL‑2 R gamma  in Mouse Splenocytes by Flow Cytometry.
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Mouse Common  gamma  Chain/IL-2 R gamma Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Common gamma Chain/IL-2 R gamma in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL-2 R alpha, rmIL‑2 R beta, recombinant human (rh) IL-2 R gamma, rhIL-15 R alpha, recombinant rat IL-2 R alpha, or recombinant canine IL-2 R alpha is observed.
Source
Monoclonal Rat IgG2B Clone # 644346
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf21-derived recombinant mouse Common gamma Chain/IL-2 R gamma
Trp23-Ala263 (predicted)
Accession # P34902
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Common  gamma  Chain/IL‑2 R gamma  in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of Common  gamma  Chain/IL‑2 R gamma in Mouse Splenocytes by Flow Cytometry. CD3+ mouse splenocytes were stained with Mouse Common  gamma  Chain/IL‑2 R gamma Monoclonal Antibody (Catalog # MAB7842, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Fluorescein-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0104B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Common gamma Chain/IL-2 R gamma

The Common gamma Chain, also known as CD132, is a transmembrane protein belonging to the hematopoietin receptor family. gamma c is a signaling component of the functional receptor complexes for IL-2, IL-4, IL-7, IL-9, and IL-15.

Long Name
IL-2 Receptor gamma
Entrez Gene IDs
3561 (Human); 16186 (Mouse)
Alternate Names
CD132 antigen; CD132; CIDX; combined immunodeficiency, X-linked; common cytokine receptor gamma chain; Common gamma Chain; common gamma-chain; cytokine receptor common subunit gamma; gamma(c); IL-2 R gamma; IL-2 receptor subunit gamma; IL2R gamma; IL-2R subunit gamma; IL2RG; IL-2RG; IMD4; interleukin 2 receptor, gamma; Interleukin-2 receptor subunit gamma; P64; SCIDX; SCIDX1; severe combined immunodeficiency

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