Mouse Crossveinless-2/CV-2 Antibody

Catalog # Availability Size / Price Qty
Crossveinless‑2/CV‑2 in Mouse Embryo.
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Product Details
Citations (5)
Supplemental Products

Mouse Crossveinless-2/CV-2 Antibody Summary

Species Reactivity
Detects mouse Crossveinless-2/CV-2 in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant human CV-2 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse Crossveinless-2/CV-2 (R&D Systems, Catalog # 2299-CV)
Accession # AAH66153
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse Crossveinless‑2/CV‑2 (Catalog # 2299-CV)
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Crossveinless-2/CV-2 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Crossveinless‑2/CV‑2 in Mouse Embryo. Crossveinless-2/CV-2 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Mouse Crossveinless-2/CV-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2299) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the epithelium surrounding the nasal cavity. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Crossveinless-2/CV-2

Crossveinless-2 (CV-2), also known as bone morphogenetic protein-binding endothelial cell precursor-derived regulator (BMPER), is a secreted chordin-like protein that modulates the BMP signaling pathway (1‑3). Mouse CV-2 is synthesized as a 685 amino acid (aa) residue precursor protein with a putative 39 aa signal peptide, five tandem chordin-like cysteine-rich (CR) domains, a partial von Willebrand factor type D domain (vWD), and a carboxyl trypsin inhibitor-like cysteine-rich domain (TIL) (1, 2, 4). Secreted CV-2 is reported to be proteolytically cleaved to generate two fragments that are disulfide-linked (1, 2). The GDPH sequence is conserved in CV-2 from other species. It is also found in multiple proteins that undergo a similar type of cleavage (5). Mouse CV-2 message is detected in many tissues, with the highest expression detected in the heart, lungs, and skin (2). It is also expressed in flk-1+ endothelial cell precursors and in primary chondrocytes (2). During embryonic development, CV-2 is expressed in the dorsal midline, regions of the telencephalon, migrating cells of the branchial neural crest and endothelial cells in the yolk sac (2). Mouse CV-2 shares 92% and 34% aa sequence identity with the human and Drosophila homologs, respectively (1, 4). Results from biochemical experiments using recombinant CV-2 show that CV-2 directly interacts with BMP-2, -4, and -6 to antagonize BMP signaling, which can regulate a wide range of differentiation processes (1, 2). In contrast, genetic data from Drosophila suggest that CV-2 potentiates BMP-signaling (6). It is possible that like TSG, CV-2 can positively and negatively modulate BMP signal transduction depending on the cell context (7).

  1. Binnerts, M.E. et al. (2004) Biochem Biophys Res Commun. 315:272.
  2. Moser, M. et al. (2003) Mol Cell Biol. 23:5664.
  3. Garcia-Abreu, J. et al. (2002) Gene 287:39.
  4. Coffinier, C. et al. (2002) Mech Dev. 119:S179.
  5. Lidell, M.E. et al. (2003) J. Biol. Chem. 278:13944.
  6. Conley, C.A. et al. (2000) Development 127:3947.
  7. Kamimura, M. et al. (2004) Developmental Dynamics 230:434.
Long Name
BMP-binding Endothelial Regulator Protein
Entrez Gene IDs
168667 (Human); 73230 (Mouse)
Alternate Names
BMP binding endothelial regulator; BMP-binding endothelial regulator precursor protein; BMP-binding endothelial regulator protein; BMPER; Bone morphogenetic protein-binding endothelial cell precursor-derived regulator; CRIM3; crossveinless 2; Crossveinless-2; CV2; CV-2; hCV2; KIAA1965; Protein crossveinless-2

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Citations for Mouse Crossveinless-2/CV-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. BMPER-induced BMP signaling promotes coronary artery remodeling.
    Authors: Dyer L, Wu Y, Moser M, Patterson C
    Dev Biol, 2014;386(2):385-94.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Crossveinless-2 is required for the relocalization of Chordin protein within the vertebral field in mouse embryos.
    Authors: Zakin L, Chang EY, Plouhinec JL, De Robertis EM
    Dev. Biol., 2010;347(1):204-15.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Development of the vertebral morphogenetic field in the mouse: interactions between Crossveinless-2 and Twisted Gastrulation.
    Authors: Zakin L, Metzinger CA, Chang EY, Coffinier C, De Robertis EM
    Dev. Biol., 2008;323(1):6-18.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  4. Crossveinless-2 Is a BMP feedback inhibitor that binds Chordin/BMP to regulate Xenopus embryonic patterning.
    Authors: Ambrosio AL, Taelman VF, Lee HX, Metzinger CA, Coffinier C, De Robertis EM
    Dev. Cell, 2008;15(2):248-60.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Western Blot
  5. BMPER is a conserved regulator of hematopoietic and vascular development in zebrafish.
    Authors: Moser M, Yu Q, Bode C, Xiong JW, Patterson C
    J. Mol. Cell. Cardiol., 2007;43(3):243-53.
    Species: Zebrafish
    Sample Types: Cell Culture Supernates
    Applications: Western Blot


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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