Mouse Crossveinless-2/CV-2 Antibody

(5 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Crossveinless-2/CV-2 in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant human CV-2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Crossveinless-2/CV-2 (R&D Systems, Catalog # 2299-CV)
    Ala39-Arg685
    Accession # AAH66153
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Crossveinless‑2/CV‑2 (Catalog # 2299-CV)
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Crossveinless‑2/CV‑2 in Mouse Embryo. Crossveinless‑2/CV‑2 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Mouse Crossveinless‑2/CV‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2299) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the epithelium surrounding the nasal cavity. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Crossveinless-2/CV-2

Crossveinless-2 (CV-2), also known as bone morphogenetic protein-binding endothelial cell precursor-derived regulator (BMPER), is a secreted chordin-like protein that modulates the BMP signaling pathway (1‑3). Mouse CV-2 is synthesized as a 685 amino acid (aa) residue precursor protein with a putative 39 aa signal peptide, five tandem chordin-like cysteine-rich (CR) domains, a partial von Willebrand factor type D domain (vWD), and a carboxyl trypsin inhibitor-like cysteine-rich domain (TIL) (1, 2, 4). Secreted CV-2 is reported to be proteolytically cleaved to generate two fragments that are disulfide-linked (1, 2). The GDPH sequence is conserved in CV-2 from other species. It is also found in multiple proteins that undergo a similar type of cleavage (5). Mouse CV-2 message is detected in many tissues, with the highest expression detected in the heart, lungs, and skin (2). It is also expressed in flk-1+ endothelial cell precursors and in primary chondrocytes (2). During embryonic development, CV-2 is expressed in the dorsal midline, regions of the telencephalon, migrating cells of the branchial neural crest and endothelial cells in the yolk sac (2). Mouse CV-2 shares 92% and 34% aa sequence identity with the human and Drosophila homologs, respectively (1, 4). Results from biochemical experiments using recombinant CV-2 show that CV-2 directly interacts with BMP-2, -4, and -6 to antagonize BMP signaling, which can regulate a wide range of differentiation processes (1, 2). In contrast, genetic data from Drosophila suggest that CV-2 potentiates BMP-signaling (6). It is possible that like TSG, CV-2 can positively and negatively modulate BMP signal transduction depending on the cell context (7).

  • References:
    1. Binnerts, M.E. et al. (2004) Biochem Biophys Res Commun. 315:272.
    2. Moser, M. et al. (2003) Mol Cell Biol. 23:5664.
    3. Garcia-Abreu, J. et al. (2002) Gene 287:39.
    4. Coffinier, C. et al. (2002) Mech Dev. 119:S179.
    5. Lidell, M.E. et al. (2003) J. Biol. Chem. 278:13944.
    6. Conley, C.A. et al. (2000) Development 127:3947.
    7. Kamimura, M. et al. (2004) Developmental Dynamics 230:434.
  • Long Name:
    BMP-binding Endothelial Regulator Protein
  • Entrez Gene IDs:
    168667 (Human); 73230 (Mouse)
  • Alternate Names:
    BMP binding endothelial regulator; BMP-binding endothelial regulator precursor protein; BMP-binding endothelial regulator protein; BMPER; Bone morphogenetic protein-binding endothelial cell precursor-derived regulator; CRIM3; crossveinless 2; Crossveinless-2; CV2; CV-2; hCV2; KIAA1965; Protein crossveinless-2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. BMPER-induced BMP signaling promotes coronary artery remodeling.
    Authors: Dyer L, Wu Y, Moser M, Patterson C
    Dev Biol, 2014;386(2):385-94.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Crossveinless-2 is required for the relocalization of Chordin protein within the vertebral field in mouse embryos.
    Authors: Zakin L, Chang EY, Plouhinec JL, De Robertis EM
    Dev. Biol., 2010;347(1):204-15.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  3. Development of the vertebral morphogenetic field in the mouse: interactions between Crossveinless-2 and Twisted Gastrulation.
    Authors: Zakin L, Metzinger CA, Chang EY, Coffinier C, De Robertis EM
    Dev. Biol., 2008;323(1):6-18.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  4. Crossveinless-2 Is a BMP feedback inhibitor that binds Chordin/BMP to regulate Xenopus embryonic patterning.
    Authors: Ambrosio AL, Taelman VF, Lee HX, Metzinger CA, Coffinier C, De Robertis EM
    Dev. Cell, 2008;15(2):248-60.
    Species: Mouse
    Sample Type: Recombinant Protein
    Application: WB
  5. BMPER is a conserved regulator of hematopoietic and vascular development in zebrafish.
    Authors: Moser M, Yu Q, Bode C, Xiong JW, Patterson C
    J. Mol. Cell. Cardiol., 2007;43(3):243-53.
    Species: Zebrafish
    Sample Type: Cell Culture Supernates
    Application: WB
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