Mouse Crossveinless-2/CV-2 Antibody
Mouse Crossveinless-2/CV-2 Antibody Summary
Ala39-Arg685
Accession # AAH66153
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Crossveinless‑2/CV‑2 in Mouse Embryo. Crossveinless-2/CV-2 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Mouse Crossveinless-2/CV-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2299) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the epithelium surrounding the nasal cavity. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Crossveinless-2/CV-2
Crossveinless-2 (CV-2), also known as bone morphogenetic protein-binding endothelial cell precursor-derived regulator (BMPER), is a secreted chordin-like protein that modulates the BMP signaling pathway (1‑3). Mouse CV-2 is synthesized as a 685 amino acid (aa) residue precursor protein with a putative 39 aa signal peptide, five tandem chordin-like cysteine-rich (CR) domains, a partial von Willebrand factor type D domain (vWD), and a carboxyl trypsin inhibitor-like cysteine-rich domain (TIL) (1, 2, 4). Secreted CV-2 is reported to be proteolytically cleaved to generate two fragments that are disulfide-linked (1, 2). The GDPH sequence is conserved in CV-2 from other species. It is also found in multiple proteins that undergo a similar type of cleavage (5). Mouse CV-2 message is detected in many tissues, with the highest expression detected in the heart, lungs, and skin (2). It is also expressed in flk-1+ endothelial cell precursors and in primary chondrocytes (2). During embryonic development, CV-2 is expressed in the dorsal midline, regions of the telencephalon, migrating cells of the branchial neural crest and endothelial cells in the yolk sac (2). Mouse CV-2 shares 92% and 34% aa sequence identity with the human and Drosophila homologs, respectively (1, 4). Results from biochemical experiments using recombinant CV-2 show that CV-2 directly interacts with BMP-2, -4, and -6 to antagonize BMP signaling, which can regulate a wide range of differentiation processes (1, 2). In contrast, genetic data from Drosophila suggest that CV-2 potentiates BMP-signaling (6). It is possible that like TSG, CV-2 can positively and negatively modulate BMP signal transduction depending on the cell context (7).
- Binnerts, M.E. et al. (2004) Biochem Biophys Res Commun. 315:272.
- Moser, M. et al. (2003) Mol Cell Biol. 23:5664.
- Garcia-Abreu, J. et al. (2002) Gene 287:39.
- Coffinier, C. et al. (2002) Mech Dev. 119:S179.
- Lidell, M.E. et al. (2003) J. Biol. Chem. 278:13944.
- Conley, C.A. et al. (2000) Development 127:3947.
- Kamimura, M. et al. (2004) Developmental Dynamics 230:434.
Product Datasheets
Citations for Mouse Crossveinless-2/CV-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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BMPER-induced BMP signaling promotes coronary artery remodeling.
Authors: Dyer L, Wu Y, Moser M, Patterson C
Dev Biol, 2014;386(2):385-94.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Crossveinless-2 is required for the relocalization of Chordin protein within the vertebral field in mouse embryos.
Authors: Zakin L, Chang EY, Plouhinec JL, De Robertis EM
Dev. Biol., 2010;347(1):204-15.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Development of the vertebral morphogenetic field in the mouse: interactions between Crossveinless-2 and Twisted Gastrulation.
Authors: Zakin L, Metzinger CA, Chang EY, Coffinier C, De Robertis EM
Dev. Biol., 2008;323(1):6-18.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Crossveinless-2 Is a BMP feedback inhibitor that binds Chordin/BMP to regulate Xenopus embryonic patterning.
Authors: Ambrosio AL, Taelman VF, Lee HX, Metzinger CA, Coffinier C, De Robertis EM
Dev. Cell, 2008;15(2):248-60.
Species: Mouse
Sample Types: Recombinant Protein
Applications: Western Blot -
BMPER is a conserved regulator of hematopoietic and vascular development in zebrafish.
Authors: Moser M, Yu Q, Bode C, Xiong JW, Patterson C
J. Mol. Cell. Cardiol., 2007;43(3):243-53.
Species: Zebrafish
Sample Types: Cell Culture Supernates
Applications: Western Blot
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