Mouse Dkk-1 Antibody

Catalog # Availability Size / Price Qty
AF1765
AF1765-SP
Dkk‑1 in Mouse Embryo.
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Product Details
Citations (9)
FAQs
Supplemental Products
Reviews (1)

Mouse Dkk-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Dkk-1 in ELISAs and Western blots. In sandwich ELISAs, less than 6% cross-reactivity with recombinant human Dkk‑1 and less than 0.1% cross-reactivity with recombinant mouse (rm) Dkk-2, rmDkk-3, and rmDkk-4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Dkk-1
Ser30-His272
Accession # O54908
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Dkk‑1 (Catalog # 1765-DK)
Immunohistochemistry
5-15 µg/mL
See below

Mouse Dkk-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Dkk‑1 Biotinylated Antibody (Catalog # BAF1765)

Standard: Recombinant Mouse Dkk-1 (Histidine-tagged) Protein (Catalog # 1765-DK)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Dkk‑1 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Dkk‑1 in Mouse Embryo. Dkk‑1 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c., cross-section through the eye) using Goat Anti-Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1765) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dkk-1

Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy (1, 2). Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Each domain contains ten cysteine residues (1‑3). Mature mouse Dkk-1 is a 40 kDa glycosylated protein that shares 86%, 96%, 83% and 82% amino acid (aa) sequence identity with human, rat, rabbit and bovine Dkk‑1, respectively. It also shares 41% and 36% aa identity with human Dkk-2 and Dkk-4, respectively. Dkk-1 and Dkk-4 are well documented antagonists of the canonical Wnt signaling pathway (1, 2). This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low‑density lipoprotein receptor-related proteins, LRP5 or LRP6 (4). Dkk-1 antagonizes Wnt by forming ternary complexes of LRP5/6 with Kremen1 or Kremen2 (4, 5). Dkk-1/LRP6/Krm2 complex internalization has been shown to downregulate Wnt signaling (4, 5). Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development (6, 7). Other sites of expression include developing neurons, hair follicles and the retina of the eye (8, 9). The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis (10). Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively (8, 11). In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Cerebral ischemia induces Dkk-1 expression, which contributes to neuronal cell death (12).

References
  1. Glinka, A. et al. (1998) Nature 391:357.  
  2. Niehrs, C. (2006) Oncogene 25:7469. 
  3. Bullock, C.M. et al. (2004) Mol. Pharmacol. 65:582. 
  4. Mao, B. et al. (2001) Nature 411:321. 
  5. Mao, B. et al. (2002) Nature 417:664.  
  6. Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
  7. Adamska, M. et al. (2004) Dev. Biol. 272:134.
  8. Li, J. et al. (2006) Bone 36:754.
  9. Verani, R. et al. (2006) J. Neurochem. 101:242.
  10. Pinzone, J.J. et al. (2009) Blood 113:517.
  11. Morvan, F. et al. (2006) J. Bone Miner. Res. 21:934.
  12. Cappuccio, I. et al. (2005) J. Neurosci. 25:2647.
Long Name
Dickkopf-1
Entrez Gene IDs
22943 (Human); 13380 (Mouse); 102123735 (Cynomolgus Monkey)
Alternate Names
dickkopf (Xenopus laevis) homolog 1; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; Dickkopf-1; dickkopf-related protein 1; Dkk1; Dkk-1; hDkk-1; SKdickkopf-1 like

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Citations for Mouse Dkk-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Ventricular, atrial, and outflow tract heart progenitors arise from spatially and molecularly distinct regions of the primitive streak
    Authors: Kenzo Ivanovitch, Pablo Soro-Barrio, Probir Chakravarty, Rebecca A. Jones, Donald M. Bell, S. Neda Mousavy Gharavy et al.
    PLOS Biology
  2. Rab7-Mediated Endocytosis Establishes Patterning of Wnt Activity through Inactivation of Dkk Antagonism
    Authors: N Kawamura, K Takaoka, H Hamada, AK Hadjantona, GH Sun-Wada, Y Wada
    Cell Rep, 2020-06-09;31(10):107733.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Platelet-rich plasma inhibits RANKL-induced osteoclast differentiation through activation of Wnt pathway during bone remodeling
    Authors: Dongyue Wang, Yajuan Weng, Shuyu Guo, Yuxin Zhang, Tingting Zhou, Mengnan Zhang et al.
    International Journal of Molecular Medicine
  4. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2017-11-13;15(1):39-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms
    Nat. Med, 2016-12-05;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  6. Induction of the Wnt antagonist Dickkopf-1 is involved in stress-induced hippocampal damage.
    Authors: Matrisciano F, Busceti CL, Bucci D, Orlando R, Caruso A, Molinaro G, Cappuccio I, Riozzi B, Gradini R, Motolese M, Caraci F, Copani A, Scaccianoce S, Melchiorri D, Bruno V, Battaglia G, Nicoletti F
    PLoS ONE, 2011-01-27;6(1):e16447.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  7. Wnt3a activates dormant c-Kit(-) bone marrow-derived cells with short-term multilineage hematopoietic reconstitution capacity.
    Authors: Trowbridge JJ, Guezguez B, Moon RT
    Stem Cells, 2010-08-01;28(8):1379-89.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  8. Inhibiting Dickkopf-1 (Dkk1) removes suppression of bone formation and prevents the development of osteolytic bone disease in multiple myeloma.
    Authors: Heath DJ, Chantry AD, Buckle CH, Coulton L, Shaughnessy JD, Evans HR, Snowden JA, Stover DR, Vanderkerken K, Croucher PI
    J. Bone Miner. Res., 2009-03-01;24(3):425-36.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Dickkopf-1 is a master regulator of joint remodeling.
    Authors: Diarra D, Stolina M, Polzer K, Zwerina J, Ominsky MS, Dwyer D, Korb A, Smolen J, Hoffmann M, Scheinecker C, van der Heide D, Landewe R, Lacey D, Richards WG, Schett G
    Nat. Med., 2007-01-21;13(2):156-63.
    Species: Human, Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot

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Mouse Dkk-1 Antibody
By Tina Yuan on 11/23/2020
Application: IHC Sample Tested: mouse bone tissue Species: Mouse