Mouse Dkk-1 Antibody

R&D Systems | Catalog # AF1765

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Dkk-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Dkk-1
Ser30-His272
Accession # O54908

Specificity

Detects mouse Dkk-1 in ELISAs and Western blots. In sandwich ELISAs, less than 6% cross-reactivity with recombinant human Dkk‑1 and less than 0.1% cross-reactivity with recombinant mouse (rm) Dkk-2, rmDkk-3, and rmDkk-4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Dkk-1 Antibody

Dkk‑1 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

Dkk‑1 in Mouse Embryo.

Dkk‑1 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c., cross-section through the eye) using Goat Anti-Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1765) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Mouse Dkk-1 ELISA Standard Curve

Mouse Dkk-1 ELISA Standard Curve

Recombinant Mouse Dkk‑1 (Histidine-tagged) (Catalog # 1765-DK) was serially diluted and captured by Goat Anti-Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1765) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1765) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Mouse Dkk-1 ELISA Standard Curve

Mouse Dkk-1 ELISA Standard Curve

Recombinant Mouse Dkk‑1 (Histidine-tagged) (Catalog # 1765-DK) was serially diluted and captured by Goat Anti-Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1765) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1765) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Detection of Dkk-1 by Immunohistochemistry

Detection of Dkk-1 by Immunohistochemistry

Rab7-Dependent Trafficking of Dkk1 Creates Wnt Signal Pattern in Peri-gastrulation Embryo(A) E6.0wild-type (n = 20) and Rab7-deficient embryos (n = 16) stained for Dkk1 (green) and sorting nexin 1 (SNX1, red). Arrows indicate colocalization of Dkk1 and SNX1 in the wild-type embryos. Outlined arrows indicate Dkk1 signals observed in Rab7 mutants, which colocalizes neither SNX1 nor Lamp2. Single-channel images are also shown.(B) E6.5 wild-type embryos (n = 21) stained for Dkk1 (green), Lamp2 (red), and mouse IgG (red). Arrows indicatethe Dkk1 signals overlapping with IgG or Lamp2.See also Videos S7 and S8. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32521258), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Dkk-1 by Immunohistochemistry

Detection of Dkk-1 by Immunohistochemistry

Accumulation of Wnt Antagonist, Dkk1 Proteins in Rab7-Deficient Embryos at Peri-gastrulation Stages(A) Schematic diagram of optical sections of a whole mounted embryo.(B) Localization of Dkk1 at E5.5 (wild type: n = 2 and rab7−/−: n = 5), E6.0 (wild type: n = 20 and rab7−/− n = 16), E6.25 (wild type: n = 10 and rab7−/−: n = 10), and E6.5 (wild type: n = 13 and rab7−/−: n = 6).(C) Elevated signal of Dkk1 in Rab7-deficient embryos between the visceral endoderm (VE) and Epi. E6.5 wild-type (n = 4) and Rab7-deficient embryos (n = 4) were stained for Dkk1 (green) and E-cadherin (red). The areas outlined by white dashed lines are magnified. Arrows indicate the Dkk1 signals outside the VE cells.(D) E6.5 wild-type (n = 10) and Rab7-deficient (n = 7) embryos stained for Dkk1 (green) and integrin beta 1 (red). The areas outlined by white dashed lines are magnified. Arrows indicate the Dkk1 signals outside the VE cells.See also Videos S1, S2, S3, S4, S5, and S6 for three-dimensional reconstitution of Dkk1 patterning. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32521258), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Dkk-1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E13.5-15.5)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Dkk‑1 (Catalog # 1765-DK)

Mouse Dkk-1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse Dkk‑1 Biotinylated Antibody (Catalog # BAF1765)
  • Standard: Recombinant Mouse Dkk-1 (Histidine-tagged) Protein (Catalog # 1765-DK)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using AF1765 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Dkk-1

Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy (1, 2). Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Each domain contains ten cysteine residues (1‑3). Mature mouse Dkk-1 is a 40 kDa glycosylated protein that shares 86%, 96%, 83% and 82% amino acid (aa) sequence identity with human, rat, rabbit and bovine Dkk‑1, respectively. It also shares 41% and 36% aa identity with human Dkk-2 and Dkk-4, respectively. Dkk-1 and Dkk-4 are well documented antagonists of the canonical Wnt signaling pathway (1, 2). This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low‑density lipoprotein receptor-related proteins, LRP5 or LRP6 (4). Dkk-1 antagonizes Wnt by forming ternary complexes of LRP5/6 with Kremen1 or Kremen2 (4, 5). Dkk-1/LRP6/Krm2 complex internalization has been shown to downregulate Wnt signaling (4, 5). Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development (6, 7). Other sites of expression include developing neurons, hair follicles and the retina of the eye (8, 9). The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis (10). Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively (8, 11). In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Cerebral ischemia induces Dkk-1 expression, which contributes to neuronal cell death (12).

References

  1. Glinka, A. et al. (1998) Nature 391:357.  
  2. Niehrs, C. (2006) Oncogene 25:7469. 
  3. Bullock, C.M. et al. (2004) Mol. Pharmacol. 65:582. 
  4. Mao, B. et al. (2001) Nature 411:321. 
  5. Mao, B. et al. (2002) Nature 417:664.  
  6. Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
  7. Adamska, M. et al. (2004) Dev. Biol. 272:134.
  8. Li, J. et al. (2006) Bone 36:754.
  9. Verani, R. et al. (2006) J. Neurochem. 101:242.
  10. Pinzone, J.J. et al. (2009) Blood 113:517.
  11. Morvan, F. et al. (2006) J. Bone Miner. Res. 21:934.
  12. Cappuccio, I. et al. (2005) J. Neurosci. 25:2647.

Long Name

Dickkopf-1

Alternate Names

Dkk1

Entrez Gene IDs

22943 (Human); 13380 (Mouse); 102123735 (Cynomolgus Monkey)

Gene Symbol

DKK1

UniProt

O54908

Additional Dkk-1 Products

Product Documents for Mouse Dkk-1 Antibody

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Product Specific Notices for Mouse Dkk-1 Antibody

For research use only

Citations for Mouse Dkk-1 Antibody

Customer Reviews for Mouse Dkk-1 Antibody (2)

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  • Mouse Dkk-1 Antibody
    Name: Tina Yuan
    Application: Immunohistochemistry
    Sample Tested: mouse bone tissue
    Species: Mouse
    Verified Customer | Posted 11/23/2020
    Mouse Dkk-1 Antibody AF1765
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: See PMID 20050968
    Species: Mouse
    Verified Customer | Posted 01/05/2015

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