Mouse Endostatin Antibody

Catalog # Availability Size / Price Qty
AF570
AF570-SP
Endostatin in Mouse Intestine.
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Product Details
Citations (5)
FAQs
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Mouse Endostatin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Endostatin in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human Endostatin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Endostatin
His1105-Lys1288
Accession # AAA19787
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.20 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Endostatin
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Endostatin in Mouse Intestine. View Larger

Endostatin in Mouse Intestine. Endostatin was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse Endostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF570) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lacteals. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endostatin

Endostatin is a fragment of collagen XVIII and functions as an angiogenesis antagonist.

Entrez Gene IDs
80781 (Human); 12822 (Mouse)
Alternate Names
antiangiogenic agent; COL18A1; collagen alpha-1(XVIII) chain; collagen, type XVIII, alpha 1; Endostatin; FLJ27325; FLJ34914; human type XVIII collagen10endostatin; KNO; KNO1MGC74745; Knobloch syndrome, type 1; KS; multi-functional protein MFP

Product Datasheets

Citations for Mouse Endostatin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Type IV collagen stimulates pancreatic cancer cell proliferation, migration, and inhibits apoptosis through an autocrine loop.
    Authors: Ohlund D, Franklin O, Lundberg E, Lundin C, Sund M
    BMC Cancer, 2013;13(0):154.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Host-derived MMP-13 exhibits a protective role in lung metastasis of melanoma cells by local endostatin production.
    Authors: Fukuda H, Mochizuki S, Abe H, Okano HJ, Hara-Miyauchi C, Okano H, Yamaguchi N, Nakayama M, D'Armiento J, Okada Y
    Br. J. Cancer, 2011;105(10):1615-24.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  3. Cationic liposome coupled endostatin gene for treatment of peritoneal colon cancer.
    Authors: Lan KL, Ou-Yang F, Yen SH, Shih HL, Lan KH
    Clin. Exp. Metastasis, 2010;27(5):307-18.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development
  4. Endostatin peptide, an inhibitor of angiogenesis, prevents the progression of peritoneal sclerosis in a mouse experimental model.
    Authors: Tanabe K, Maeshima Y, Ichinose K, Kitayama H, Takazawa Y, Hirokoshi K, Kinomura M, Sugiyama H, Makino H
    Kidney Int., 2007;71(3):227-38.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Platelet-derived growth factor-BB controls epithelial tumor phenotype by differential growth factor regulation in stromal cells.
    Authors: Lederle W, Stark HJ, Skobe M, Fusenig NE, Mueller MM
    Am. J. Pathol., 2006;169(5):1767-83.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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