Mouse Endostatin Antibody Summary
Accession # AAA19787
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Endostatin in Mouse Intestine. Endostatin was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse Endostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF570) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lacteals. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Endostatin is a fragment of collagen XVIII and functions as an angiogenesis antagonist.
Citations for Mouse Endostatin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 5
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Type IV collagen stimulates pancreatic cancer cell proliferation, migration, and inhibits apoptosis through an autocrine loop.
Authors: Ohlund D, Franklin O, Lundberg E, Lundin C, Sund M
BMC Cancer, 2013;13(0):154.
Sample Types: Whole Cells
Host-derived MMP-13 exhibits a protective role in lung metastasis of melanoma cells by local endostatin production.
Authors: Fukuda H, Mochizuki S, Abe H, Okano HJ, Hara-Miyauchi C, Okano H, Yamaguchi N, Nakayama M, D'Armiento J, Okada Y
Br. J. Cancer, 2011;105(10):1615-24.
Sample Types: Tissue Homogenates
Applications: Western Blot
Cationic liposome coupled endostatin gene for treatment of peritoneal colon cancer.
Authors: Lan KL, Ou-Yang F, Yen SH, Shih HL, Lan KH
Clin. Exp. Metastasis, 2010;27(5):307-18.
Sample Types: Serum
Applications: ELISA Development
Endostatin peptide, an inhibitor of angiogenesis, prevents the progression of peritoneal sclerosis in a mouse experimental model.
Authors: Tanabe K, Maeshima Y, Ichinose K, Kitayama H, Takazawa Y, Hirokoshi K, Kinomura M, Sugiyama H, Makino H
Kidney Int., 2007;71(3):227-38.
Sample Types: Whole Tissue
Platelet-derived growth factor-BB controls epithelial tumor phenotype by differential growth factor regulation in stromal cells.
Authors: Lederle W, Stark HJ, Skobe M, Fusenig NE, Mueller MM
Am. J. Pathol., 2006;169(5):1767-83.
Sample Types: Whole Tissue
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Cryosections from PLP immersion fixed tissues were stained for Endostatin (1:50) for 4h @ RT. After washing, tissue was stained with Donkey anti-Goat alexa546 2ndary antibody (1:500) for 1h at RT. E-cadherin in green, Endostatin in red, nuclear staining (Hoechst) in blue.