Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Porcine, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry, ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Endostatin Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Endostatin
His1105-Lys1288
Accession # AAA19787

Specificity

Detects mouse Endostatin in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human Endostatin is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Endostatin Antibody

Endostatin antibody in Mouse Intestine by Immunohistochemistry (IHC-Fr).

Endostatin in Mouse Intestine.

Endostatin was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse Endostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF570) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lacteals. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse Endostatin Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse intestine

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Endostatin

Reviewed Applications

Read 4 reviews rated 4.8 using AF570 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Endostatin

Endostatin is a fragment of collagen XVIII and functions as an angiogenesis antagonist.

Alternate Names

COL18A1

Entrez Gene IDs

80781 (Human); 12822 (Mouse)

Gene Symbol

COL18A1

UniProt

AAA19787

Additional Endostatin Products

Product Documents for Mouse Endostatin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Endostatin Antibody

For research use only

Related Research Areas

Extracellular Matrix Molecules

Citations for Mouse Endostatin Antibody

Customer Reviews for Mouse Endostatin Antibody (4)

4.8 out of 5
4 Customer Ratings
5 Stars
75%
4 Stars
25%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Mouse Endostatin Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 4 of 4 reviews Showing All
Filter By:
Clear Filters X
  • Mouse Endostatin Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Retina
    Species: Mouse
    Verified Customer | Posted 11/22/2021
    Mouse Endostatin Antibody AF570
  • Mouse Endostatin Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Heart tissue
    Species: Mouse
    Verified Customer | Posted 07/16/2021
    Mouse Endostatin Antibody AF570
  • Mouse Endostatin Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Kidney tissue
    Species: Mouse
    Verified Customer | Posted 06/30/2021
    Staining for endostatin in proximal and distal tubules (mouse kidney)
    Mouse Endostatin Antibody AF570
  • Mouse Endostatin Antibody
    Name: Efstathios Stamatiades
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adult small intestine
    Species: Mouse
    Verified Customer | Posted 07/09/2019
    Cryosections from PLP immersion fixed tissues were stained for Endostatin (1:50) for 4h @ RT. After washing, tissue was stained with Donkey anti-Goat alexa546 2ndary antibody (1:500) for 1h at RT. E-cadherin in green, Endostatin in red, nuclear staining (Hoechst) in blue.
    Mouse Endostatin Antibody AF570

There are no reviews that match your criteria.

Showing  1 - 4 of 4 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies