Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all EPCR Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse EPCR
Ala17-Ser214
Accession # Q64695

Specificity

Detects mouse EPCR in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human EPCR is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse EPCR Antibody

Detection of Mouse EPCR antibody by Western Blot.

Detection of Mouse EPCR by Western Blot.

Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse EPCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2749) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EPCR at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of EPCR antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry.

Detection of EPCR in bEnd.3 Mouse Cell Line by Flow Cytometry.

bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse EPCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2749, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

EPCR antibody in bEnd by Immunocytochemistry (ICC).3 Mouse Cell Line by Immunocytochemistry (ICC).

EPCR in bEnd.3 Mouse Cell Line.

EPCR was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse EPCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2749) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse EPCR Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: bEnd.3 mouse endothelioma cell line

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed bEnd.3 mouse endothelioma cell line

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse EPCR, see our available Western blot detection antibodies

Western Blot

0.5 µg/mL
Sample: bEnd.3 mouse endothelioma cell line

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EPCR

Protein C is a vitamin K-dependent serine protease that plays a major role in blood coagulation. Binding of Protein C to EPCR (CD201) leads to the proteolytic activation of PAR1 (protease-activated receptor 1) on endothelial cells and subsequent up-regulation of Protein C-induced genes. EPCR is a type I transmembrane glycoprotein in the CD1/MHC family. It is expressed most strongly in the endothelial cells of arteries and veins in heart and lung. Membrane bound EPCR is released by metalloproteolytic cleavage to generate the soluble receptor. The extracellular domain of human and mouse EPCR shares approximately 61% amino acid sequence homology.

Long Name

Endothelial Protein C Receptor

Alternate Names

CCD41, CD201, PROCR

Entrez Gene IDs

10544 (Human); 19124 (Mouse)

Gene Symbol

PROCR

UniProt

Q64695

Additional EPCR Products

Product Documents for Mouse EPCR Antibody

Certificate of Analysis

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Product Specific Notices for Mouse EPCR Antibody

For research use only

Citations for Mouse EPCR Antibody

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Protocols

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