Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FGFR5/FGFRL1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse FGF R5 beta
Ala21-Pro374
Accession # Q91V87

Specificity

Detects mouse FGF R5 beta in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) FGF R1 alpha  (IIIb), recombinant mouse (rm) FGF R2 beta  (IIIc), rmFGF R3 alpha  (IIIb), rhFGF R3 alpha  (IIIc), rhFGF R1 beta  (IIIc), rmFGF R2 beta  (IIIb), rhFGF R1 alpha  (IIIc), rmFGF R2 alpha  (IIIc), rhFGF R1 beta  (IIIb), rmFGF R2 alpha (IIIb), rmFGF R2, and rmFGF R4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse FGFR5/FGFRL1 Antibody

FGF R5/FGFRL1 antibody in Mouse Lung by Immunohistochemistry (IHC-Fr).

FGF R5/FGFRL1 in Mouse Lung.

FGF R5/FGFRL1 was detected in perfusion fixed frozen sections of mouse lung using Goat Anti-Mouse FGF R5/FGFRL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1899) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane and cytoplasm of alveolar cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse FGFR5/FGFRL1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse liver, lung, lymph node, and thymus

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse FGF R5/FGFRL1 Fc Chimera (Catalog # 1899-FR)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGFR5/FGFRL1

Fibroblast growth factor receptor 5 (FGF R5), also known as FGRL1, is a 65 kDa transmembrane member of the FGF receptor family (1). Mature mouse FGF R5 consists of a 354 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 134 aa cytoplasmic domain (2). FGF R5 is distinct from other FGF receptor family members in that it does not contain a cytoplasmic tyrosine kinase domain (2, 3). Within the ECD, mouse FGF R5 shares 94% and 98% aa sequence identity with human and rat FGF R5, respectively. Alternate splicing results in an isoform that lacks the first Ig-like domain (2). FGF R5 is widely expressed, and expression of two species of mRNA in cartilage and pancreas may indicate the presence of both splice forms (2-6). Both the full length and truncated isoforms of FGF R5 bind FGF basic; the full length form additionally binds to heparin (2, 6). FGF R5 may function as a decoy receptor by binding FGF but not transducing its mitogenic signals (6).

References

  1. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  2. Sleeman, M. et al. (2001) Gene 271:171.
  3. Kim, I. et al. (2001) Biochim. Biophys. Acta 1518:152.
  4. Antoine, M. et al. (2005) Growth Factors 23:87.
  5. Wiedemann, M. et al. (2000) Genomics 69:275.
  6. Trueb, B. et al. (2003) J. Biol. Chem. 278:33857.

Long Name

Fibroblast Growth Factor Receptor-like 1

Alternate Names

FGF R5, FGFRL1

Entrez Gene IDs

53834 (Human); 116701 (Mouse)

Gene Symbol

FGFRL1

UniProt

Q91V87

Additional FGFR5/FGFRL1 Products

Product Documents for Mouse FGFR5/FGFRL1 Antibody

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Product Specific Notices for Mouse FGFR5/FGFRL1 Antibody

For research use only

Citations for Mouse FGFR5/FGFRL1 Antibody

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Protocols

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