Mouse Frizzled-7 Antibody Summary
Accession # Q61090
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse Frizzled‑7 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Frizzled‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF198) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Frizzled‑7 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Frizzled‑7 in Embryonic Mouse Gastrointestinal Tract.
Frizzled‑7 in Embryonic Mouse Gastrointestinal Tract.Frizzled‑7 was detected in immersion fixed frozen sections of embryonic mouse gastrointestinal tract (11 d.p.c.) using 15 µg/mL Goat Anti-Mouse Frizzled‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF198) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance (1). Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway (2). The predicted structure of Frizzled proteins is similar among all family members, containing a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain (CRD), a variable-length linker region, a seven-pass transmembrane region, and a variable-length C-terminal cytoplasmic domain. The CRD, which comprises the Wnt binding site, spans about 130 amino acid residues and contains ten invariant cysteine residues. Mouse Frizzled-7 contains 572 amino acid residues and shares 99% identity with the human orthologue in the CRD (3, 4). Frizzled-7 mRNA has been detected in relatively large amounts in adult skeletal muscle, plancenta and heart, and fetal kidney and lung (3). Several Frizzled-dependent signaling pathways exist (2). Their activation depends on the Wnt ligand and the cell context. Frizzled-7 can activate canonical Wnt/ beta -Catenin signaling, as well as a non-canonical pathway involved in tissue morphogenesis (4, 5).
- Wodarz, A. and R. Nusse (1998) Annu. Rev. Cell Dev. Biol. 14:59.
- Hsieh, J-C. (2004) Front. Biosci. 9:1333.
- Sagara, N. et al. (1998) Biochem. Biophys. Res. Commun. 252:117.
- Sheldahl, L.C. et al. (1999) Curr. Biol. 9:695.
- Medina, A. et al. (2000) Mech. Dev. 92:227.
Citations for Mouse Frizzled-7 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 5
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Cftr Modulates Wnt/?-Catenin Signaling and Stem Cell Proliferation in Murine Intestine
Authors: AM Strubberg, J Liu, NM Walker, CD Stefanski, RJ MacLeod, ST Magness, LL Clarke
Cell Mol Gastroenterol Hepatol, 2018;5(3):253-271.
Sample Types: Whole Tissue
Frizzled 7 and PIP2 binding by syntenin PDZ2 domain supports Frizzled 7 trafficking and signalling
Nat Commun, 2016;7(0):12101.
Sample Types: Whole Cells
Wnt-5a/Frizzled9 receptor signaling through the G?o/G?? complex regulates dendritic spine formation
J Biol Chem, 2016;0(0):.
Sample Types: Cell Lysates
Applications: Western Blot
The postsynaptic density 95/disc-large/zona occludens protein syntenin directly interacts with frizzled 7 and supports noncanonical Wnt signaling.
Authors: Luyten A, Mortier E, Van Campenhout C, Taelman V, Degeest G, Wuytens G, Lambaerts K, David G, Bellefroid EJ, Zimmermann P
Mol. Biol. Cell, 2008;19(4):1594-604.
Sample Types: Whole Cells
Regulation of endothelial cell cytoskeletal reorganization by a secreted frizzled-related protein-1 and frizzled 4- and frizzled 7-dependent pathway: role in neovessel formation.
Authors: Dufourcq P, Leroux L, Ezan J, Descamps B, Lamaziere JM, Costet P, Basoni C, Moreau C, Deutsch U, Couffinhal T, Duplaa C
Am. J. Pathol., 2007;172(1):37-49.
Sample Types: Whole Cells
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