Mouse GFR alpha-3/GDNF R alpha-3 Antibody

R&D Systems | Catalog # AF2645

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Neutralization, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all GFR alpha-3/GDNF R alpha-3 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse GFR alpha -3/GDNF R alpha -3
Glu34-Arg379
Accession # AAB70931

Specificity

Detects mouse GFR alpha -3/GDNF R alpha -3 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human GFR alpha -3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse GFR alpha-3/GDNF R alpha-3 Antibody

GFRa‑3/GDNF Ra‑3 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

GFR alpha ‑3/GDNF R alpha ‑3 in Mouse Embryo.

GFRa‑3/GDNF Ra‑3 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using 15 µg/mL Goat Anti-Mouse GFRa‑3/GDNF Ra‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2645) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to developing dorsal root ganglion and spinal cord. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse GFR alpha-3/GDNF R alpha-3 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (15 d.p.c.)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse GFR alpha ‑3/GDNF R alpha ‑3 Fc Chimera (Catalog # 2645-FR)

Reviewed Applications

Read 2 reviews rated 5 using AF2645 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GFR alpha-3/GDNF R alpha-3

Mouse glial cell line-derived neurotrophic factor (GDNF) family receptor alpha 3 (GFR alpha -3) is a member of the GDNF family of receptors. It is one of four known members, all of which contain three conserved cysteine repeats (1, 3, 4). It is synthesized as a 397 aa precursor, with a hydrophobic signal sequence of 28 aa, a 343 aa mature segment, and a propeptide segment of 26 aa that is removed in the mature protein. The mature globular membrane glycoprotein has an approximate molecular weight of 50 kDa (5), contains three potential N-linked glycosylation sites, and a hydrophobic stretch of residues at its COOH terminus that comprises a GPI linkage sequence (2, 3, 5). Mouse GFR alpha -3 shares 81% aa sequence identity with human GFR alpha -3. High-level expression of GFR alpha -3 is observed only during early stages of neurogenesis in the central nervous system and in developing and adult peripheral nerves, organs, and ganglia (2, 3, 5, 7). The expression and proportions of GFR alpha -3 are closely linked to those of the Ret receptor tyrosine kinase, particularly in the trigeminal ganglion, pituitary gland, thymus, lung, and duodenum (3). GFR alpha -3 associates with Ret to form the receptor complex for the GDNF family ligand artemin, which, in turn, activates the complex (1, 6, 7). The activated complex’s signal is required for the development and maintenance of superior cervical ganglion (SCG), specifically the rostral migration of SCG precursors between embryonic days 11.5 and 14.5, and the survival of SCG neurons after birth (8).

References

  1. Xinquan, W. et al. (2006) Structure 14:1083.
  2. Worby, C. et al. (1998) J. Biol. Chem. 273:3502.
  3. Naveilhan, P. et al. (1998) Proc. Natl. Acad. Sci. USA 95:1295.
  4. Nomoto, S. et al. (1998) Biochem. Biophys. Res. Commun. 244:849.
  5. Baloh, R. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
  6. Saarma, M. (2000) Eur. J. Biochem. 267:6968.
  7. Baloh, R. et al. (1998) Neuron 21:1291.
  8. Nishino, J et al. (1999) Neuron 23:725.

Long Name

Glial Cell line-derived Neurotrophic Factor Receptor alpha 3

Alternate Names

GDNF R alpha-3, GFR alpha3, GFRa-3, GFRA3

Entrez Gene IDs

2676 (Human); 14587 (Mouse)

Gene Symbol

GFRA3

UniProt

AAB70931

Additional GFR alpha-3/GDNF R alpha-3 Products

Product Documents for Mouse GFR alpha-3/GDNF R alpha-3 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse GFR alpha-3/GDNF R alpha-3 Antibody

For research use only

Related Research Areas

GDNF Family

Citations for Mouse GFR alpha-3/GDNF R alpha-3 Antibody

Customer Reviews for Mouse GFR alpha-3/GDNF R alpha-3 Antibody (2)

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  • Mouse GFR alpha -3/GDNF R alpha -3 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Dorsal root ganglion
    Species: Mouse
    Verified Customer | Posted 07/21/2021
    Mouse GFR alpha-3/GDNF R alpha-3 Antibody AF2645
  • Mouse GFR alpha -3/GDNF R alpha -3 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Brain (hippocampus)
    Species: Mouse
    Verified Customer | Posted 04/28/2021
    Mouse GFR alpha-3/GDNF R alpha-3 Antibody AF2645

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