Mouse GITR/TNFRSF18 Antibody

Catalog # Availability Size / Price Qty
AF524
AF524-SP

Save Up to 40% on RUO Reagents with BIOSPRING24 (See Details)

Product Details
Citations (6)
FAQs
Supplemental Products
Reviews

Mouse GITR/TNFRSF18 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse GITR/TNFRSF18 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross‑reactivity with recombinant human (rh) GITR is observed, less than 2% cross‑reactivity with recombinant mouse (rm) 4-1BB, rmCD27, rmCD30, rmCD40, rhDR6, rmFas, rhHVEM, rmLTR beta, rmTNF RI, and rmTNF RII is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse GITR/TNFRSF18
Ser22-His153
Accession # Q8C4K3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse GITR/TNFRSF18 Fc Chimera (Catalog # 524-GR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GITR/TNFRSF18

GITR (glucocorticoid-induced tumor necrosis factor receptor; also named AITR) is a member of the co‑stimulatory subset of the TNF receptor superfamily (1, 2). In mouse, the GITR gene is composed of five exons and encodes multiple length isoforms that arise from alternate splicing. The “standard”, or first reported isoform is a type I transmembrane protein, 228 amino acids (aa) in length that contains a 19 aa signal sequence, a 134 aa extracellular region, a 21 aa transmembrane segment, and a 54 aa cytoplasmic domain. The extracellular region contains four potential N-linked glycosylation sites plus three cysteine-rich pseudorepeats of about 40 aa each (3, 4). The extracellular regions of mouse and human are 57% aa identical. The cytoplasmic domain has a P-x-Q/E-E motif that is known to associate with TRAF2. This is a common characteristic of TNFRSF members with co‑stimulatory functions (4). Three other mouse GITR isoforms (B, C and D) have been reported (5). All share the same N-terminal 101 of 134 aa in the extracellular region (including pseudorepeats #1, #2 and one-half of #3). Isoform D diverges at aa #101 and continues for another 12 aa for a total length of 113 aa. This is a naturally-occurring soluble form. Isoforms B and C show splicing in their cytoplasmic tails that creates cytoplasmic domains of 118 aa and 46 aa, respectively. In both the B and C isoforms, the TRAF2 binding site is spliced out, with a p56lck binding site inserted in isoform B (4). Given its membership in the TNFRSF, it likely functions as a trimer on the cell surface (2). GITR is predominantly expressed on CD4+CD25+ regulatory T cells (Treg) and naïve CD8+ and CD4+ CD25- T cells, where its expression is up-regulated after antigen-driven activation. GITR activation provides co‑stimulatory signals for activated CD4+ CD25- T cells to enhance cell proliferation and augment cytokine production (IL-2, IL-4, IFN-gamma ). On CD4+ CD25+ Treg cells, GITR activation provides co‑stimulatory signals to induce proliferation, setting Treg cells in an active/hyperproliferactive state (6‑8).

References
  1. Kwon, B. et al. (2003) Exp. Mol. Med. 35:8. 
  2. Croft, M. (2003) Nat. Rev. Immunol. 3:609. 
  3. Nocentini, G. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216.
  4. Nocentini, G. et al. (2000) DNA Cell Biol. 19:205. 
  5. Nocentini, G. et al. (2000) Cell Death Differ. 7:408.
  6. Tone, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:15059.
  7. Ji, H. et al. (2004) J. Immunol. 172:5823.
  8. Stephens, G.L. et al. (2004) 173:5008.
Long Name
Glucocorticoid Induced TNF Receptor Family Related Gene
Entrez Gene IDs
8784 (Human); 21936 (Mouse); 500598 (Rat); 102146362 (Cynomolgus Monkey)
Alternate Names
Activation-inducible TNFR family receptor; AITR; AITRTNF receptor superfamily activation-inducible protein; CD357 antigen; CD357; GITR; GITR-D; GITRtumor necrosis factor receptor superfamily member 18; Glucocorticoid-induced TNFR-related protein; TNFRSF18; tumor necrosis factor receptor superfamily, member 18

Product Datasheets

You must select a language.

x

Citations for Mouse GITR/TNFRSF18 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Filter by:

  1. Enterococcus faecalis promotes a migratory and invasive phenotype in colon cancer cells
    Authors: Ashley J. Williamson, Rick Jacobson, J.B. van Praagh, Sara Gaines, Hyun Y. Koo, Brandon Lee et al.
    Neoplasia
  2. Multiple mechanisms for TRAF3-mediated regulation of the T cell costimulatory receptor GITR
    Authors: H Li, BS Hostager, T Arkee, GA Bishop
    The Journal of Biological Chemistry, 2021-08-18;0(0):101097.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Glucocorticoid-induced tumour necrosis factor receptor-related protein: a key marker of functional regulatory T cells.
    Authors: Ronchetti S, Ricci E, Petrillo M, Cari L, Migliorati G, Nocentini G, Riccardi C
    J Immunol Res, 2015-04-15;2015(0):171520.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: IHC
  4. Interleukin-2 gene variation impairs regulatory T cell function and causes autoimmunity.
    Authors: Yamanouchi J, Rainbow D, Serra P, Howlett S, Hunter K, Garner VE, Gonzalez-Munoz A, Clark J, Veijola R, Cubbon R, Chen SL, Rosa R, Cumiskey AM, Serreze DV, Gregory S, Rogers J, Lyons PA, Healy B, Smink LJ, Todd JA, Peterson LB, Wicker LS, Santamaria P
    Nat. Genet., 2007-02-04;39(3):329-37.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  5. GITR, a member of the TNF receptor superfamily, is costimulatory to mouse T lymphocyte subpopulations.
    Authors: Ronchetti S, Zollo O, Bruscoli S, Agostini M, Bianchini R, Nocentini G, Ayroldi E, Riccardi C
    Eur. J. Immunol., 2004-03-01;34(3):613-22.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Foxp3 programs the development and function of CD4+CD25+ regulatory T cells.
    Authors: Fontenot, Jason D, Gavin, Marc A, Rudensky, Alexande
    Nat Immunol, 2003-03-03;4(4):330-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse GITR/TNFRSF18 Antibody

There are currently no reviews for this product. Be the first to review Mouse GITR/TNFRSF18 Antibody and earn rewards!

Have you used Mouse GITR/TNFRSF18 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review