Mouse Glypican 5 Antibody

Catalog # Availability Size / Price Qty
AF2689
AF2689-SP

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Mouse Glypican 5 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Glypican 5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant human (rh) Glypican 5 is observed and less than 1% cross-reactivity with recombinant mouse Glypican 2, rhGlypican 3, and rhGlypican 6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Glypican 5
Gly26-Gly549
Accession # Q8CAL5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Glypican 5 (Catalog # 2689-G5)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glypican 5

The glypicans (glypiated proteoglycans) are a small multigene family of GPI-linked proteoglycans that likely play a key role in embryonic morphogenesis (1‑4). There are six known mammalian glypicans. They all share a common-sized protein core of 60‑70 kDa, an N-terminus which likely forms a compact globular domain, 14 conserved cysteines that form multiple intrachain disulfide bonds, and a number of C-terminal N- and O-linked carbohydrate attachment sites. Based on exon organization and the location of O-linked glycosylation sites, at least two subfamilies of glypicans are known, with one subfamily containing glypicans-1, 2, 4, and 6, and another subfamily containing glypicans-3 and 5 (3, 5). Mouse glypican-5 (GPC-5) is synthesized as a 572 amino acid (aa) preproprecursor that contains a 24 aa signal sequence and, based on human GPC-5, a 532 aa mature segment plus a 16 aa C-terminal prosegment (6, 7). There are four potential N-linked, and four potential O-linked sites for glycosylation or glycanation. GPC-5 is believed to contain 6‑7 kDa of glycosylation and at least 55 kDa of proteoglycan. This is based on an assumption of the presence of the least one heparan sulfate chain of 36 kDa and one chrondroitin sulfate chain of 17 kDa (8, 9). When added to the core molecular weight of 59 kDa, it may be approximately 120 kDa in the native state. The actual size of neither mouse nor human GPC-5 has been reported, and the suggestion of a chrondroitin sulfate modification is based on the expression of human GPC-5 in COS-7 cells (8). Mouse to human, there is 88% aa identity over the mature region. Cells known to express GPC-5 are principally embryonic in nature, and include neurons and mesenchyme (1, 8). As a glypican family member, it may facilitate heparin-binding growth factor signaling and polyamine uptake into expressing cells (10, 11).

References
  1. Song, H.H. and J. Filmus (2002) Biochim. Biophys. Acta 1573:241.
  2. Filmus, J. (2001) Glycobiology 11:19R.
  3. De Cat, B. and G. David (2001) Semin. Cell Dev. Biol. 12:117.
  4. Filmus, J. and S.B. Selleck (2001) J. Clin. Invest.108:497.
  5. Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.
  6. GenBank Accession # Q8CAL5.
  7. Veugelers, M. et al. (1997) Genomics 40:24.
  8. Saunders, S. et al. (1997) Dev. Biol. 19:78.
  9. Rapraeger, A. et al. (1985) J. Biol. Chem. 260:11046.
  10. Fransson, L-A. et al. (2004) Cell Mol. Life Sci. 61:1016.
  11. Fransson, L-A. (2003) Int. J. Biochem. Cell Biol. 35:125.
Entrez Gene IDs
2262 (Human); 103978 (Mouse)
Alternate Names
bA93M14.1; Glypican 5; glypican proteoglycan 5; glypican-5; GPC5

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