Mouse IgG3 Antibody Summary
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse IgG3Primary Antibodies by Western Blot. Western blot shows lysates of HepG2 and Jurkat cell lines. PVDF membrane was probed with anti-HSP90 (Catalog # MAB3286, Mouse IgG2Bisotype), anti-HSP60 (Catalog # MAB1800, Mouse IgG2Bisotype), anti-GAPDH (Catalog # MAB5718,Mouse IgG1isotype), anti-Peroxiredoxin 6 (Catalog # MAB3490, Mouse IgG3isotype), and anti-SOD1 (Catalog # MAB3418, Mouse IgG2Aisotype). The left panel was then probed with 0.5 µg/mL of Rabbit Anti-Mouse IgG3(Catalog # MAB9675) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific bands was detected for IgG3isotype antibody at approximately 26 kDa (as indicated). The right panel was probed with HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018) but not Rabbit Anti-Mouse IgG2BMonoclonal Antibody (Catalog # MAB9675). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse IgG3Primary Antibody by Anti-Mouse IgG3-specific Secondary Antibody. Human HT-29 cell line was stained with Mouse anti-Human TSPAN-1 Monoclonal Antibody (Catalog # MAB9186, Mouse IgG3isotype), followed by Rabbit anti-Mouse IgG3-specific secondary antibody (Catalog # MAB9675, filled histogram), Rabbit anti-Mouse IgG1-specific (Catalog # MAB9627, blue line), Rabit anti-Mouse IgG2A-specific (Catalog # MAB0033, red line), or Rabbit anti-Mouse IgG2B-specific (Catalog # MAB9636, black line) secondary antibody, then PE-conjugated Goat anti-Rabbit secondary antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
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