Mouse IgG2B Antibody

Recombinant Monoclonal Antibody
    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IgG2B in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with mouse IgG1, mouse IgG2A, and mouse IgG3 was observed.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 2215A
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    Mouse IgG
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse IgG2B Primary Antibodies by Western Blot. Western blot shows lysates of HepG2 and Jurkat cell lines. PVDF membrane was probed with anti-HSP90 (Catalog # MAB3286, Mouse IgG2B isotype), anti-HSP60 (Catalog # MAB1800, Mouse IgG2B isotype), anti-GAPDH (Catalog # MAB5718,Mouse IgG1 isotype), anti-Peroxiredoxin 6 (Catalog # MAB3490, Mouse IgG3 isotype), and anti-SOD1 (Catalog # MAB3418, Mouse IgG2A isotype). The left panel was then probed with 0.5 µg/mL of Rabbit Anti-Mouse IgG2B (Catalog # MAB9636) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for IgG2B isotype antibodies at approximately 60 and 90 kDa (as indicated). The right panel was probed with HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018) but not Rabbit Anti-Mouse IgG2B Monoclonal Antibody (Catalog # MAB9636). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse IgG2B PrimaryAntibody by Anti-Mouse IgG2B-specific Secondary Antibody. Human PBMC lymphocytes were stained with Mouse anti-HumanCD3 epsilon Monoclonal Antibody (Catalog # MAB100, Mouse IgG1 isotype), Mouseanti-Human CD4 Monoclonal Antibody (Catalog # MAB3791, Mouse IgG2A isotype),or Mouse anti-Human CD8 alpha Monoclonal Antibody (Catalog # MAB1509, Mouse IgG2B isotype), followedby Rabbit anti-Mouse IgG2B-specific secondary antibody (Catalog #MAB9636) then PE-conjugated Goat anti-Rabbit secondary antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G2B Fc
  • Alternate Names:
    IgG2B

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 46  
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Normal Rabbit IgG Control

Ctrl , CyTOF-ready MAB1050  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 34  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow , IHC , ICC NL004 10
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL006 4
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Rabbit IgG Antibody

WB AF008 1
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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