Mouse IL-10 R alpha Antibody

Catalog #: AF-474-NA
9 Citations Datasheet / COA / SDS

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AF-474-NA
AF-474-SP

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Cell Proliferation Induced by IL‑10 and Neutralization by Mouse IL‑10 R alpha Antibody.

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Citations (9)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-10 R alpha Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-10 R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 25% cross-reactivity with recombinant human IL-10 R alpha is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑10 R alpha
Thr24-Asn238 (Lys188Arg and Glu222Gly)
Accession # Q61727

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IL‑10 R alpha (Catalog # 474-MR)

Neutralization

Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line [Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507]. The Neutralization Dose (ND₅₀) is typically 1-3 µg/mL in the presence of 1 ng/mL Recombinant Mouse IL‑10.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL‑10 and Neutralization by Mouse IL‑10 R alpha Antibody. Recombinant Mouse IL‑10 (Catalog # 417-ML) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑10 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑10 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-474-NA). The ND₅₀ is typically 1-3 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10 R alpha

IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), is a potent immunosuppressant of macrophage functions. IL-10 is also a pleiotropic cytokine with multiple immunostimulatory as well as immunosuppressive effects on a variety of other cell types. IL-10 binds specifically and with high affinity to cell-surface receptors. Mouse and human cDNA clones encoding the ligand-binding IL-10 receptor (IL-10 R) have been isolated. The IL-10 R mRNA has been detected in all cell types that are known to respond to IL-10.

Human and mouse IL-10 receptors are structurally related to the IFN-gamma receptor. These receptors are members of the class II subgroup of the cytokine receptor superfamily. The deduced amino acid sequence of human IL-10 R is approximately 60% identical to mouse IL-10 R. Although human IL-10 has cross-species activities and is active on mouse cells, mouse IL-10 is species-specific in its actions and does not bind to the human IL-10 receptor. The human IL-10 R gene has been mapped to chromosome 11q23.3. Recombinant IL-10 soluble receptor, consisting of the extracellular domain of IL-10 R, binds IL-10 with high affinity in solution and is a potent IL-10 antagonist.

Long Name

Interleukin 10 Receptor alpha

Entrez Gene IDs

3587 (Human); 16154 (Mouse); 102127536 (Cynomolgus Monkey)

Alternate Names

CD210 antigen; CDw210a; HIL-10R; IBD28; IL-10 R alpha; IL-10 receptor subunit alpha; IL10R alpha; IL-10R subunit 1; IL-10R1; IL10RA; IL-10Ra; IL10RIL-10R subunit alpha; interleukin 10 receptor, alpha; interleukin-10 receptor alpha chain; Interleukin-10 receptor subunit 1; interleukin-10 receptor subunit alpha

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse IL-10 R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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IL-10 normalizes aberrant amygdala GABA transmission and reverses anxiety-like behavior and dependence-induced escalation of alcohol intake
Authors: RR Patel, SA Wolfe, M Bajo, S Abeynaike, A Pahng, V Borgonetti, S D'Ambrosio, R Nikzad, S Edwards, S Paust, AJ Roberts, M Roberto
Prog Neurobiol, 2020-11-13;0(0):101952.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Involvement of Interleukin-10 in Analgesia of Electroacupuncture on Incision Pain
Authors: WJ Dai, JL Sun, C Li, W Mao, YK Huang, ZQ Zhao, YQ Zhang, N Lü
Evid Based Complement Alternat Med, 2019-10-30;2019(0):8413576.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Re-evaluation of IL-10 signaling reveals novel insights on the contribution of the intracellular domain of the IL-10R2 chain
Authors: RHP Wilbers, DR van Raaij, LB Westerhof, J Bakker, G Smant, A Schots
PLoS ONE, 2017-10-10;12(10):e0186317.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
The Dendritic Cell Receptor DNGR-1 Promotes the Development of Atherosclerosis in Mice
Authors: Y Haddad, C Lahoute, M Clément, L Laurans, S Metghalchi, Z Lynda, A Giraud, X Loyer, M Vandestien, J Wain-Hobso, B Esposito, S Potteaux, H Ait-Oufell, A Tedgui, Z Mallat, S Taleb
Circ. Res., 2017-06-12;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Alternative activation-skewed microglia/macrophages promote hematoma resolution in experimental intracerebral hemorrhage
Authors: CF Chang, J Wan, Q Li, SC Renfroe, NM Heller, J Wang
Neurobiol. Dis., 2017-03-30;103(0):54-69.
Species: Mouse
Sample Types: Whole Tissue
Applications: Functional Assay
Mesenchymal Stem Cell-Educated Macrophages Ameliorate LPS-Induced Systemic Response
Authors: Yaoqin Hu
Mediators Inflamm, 2016-07-27;2016(0):3735452.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
IL4I1 Is a Novel Regulator of M2 Macrophage Polarization That Can Inhibit T Cell Activation via L-Tryptophan and Arginine Depletion and IL-10 Production.
Authors: Yue Y, Huang W, Liang J, Guo J, Ji J, Yao Y, Zheng M, Cai Z, Lu L, Wang J
PLoS ONE, 2015-11-24;10(11):e0142979.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Neuronal synapse formation induced by microglia and interleukin 10.
Authors: Lim S, Park E, You B, Jung Y, Park A, Park S, Lee J
PLoS ONE, 2013-11-22;8(11):e81218.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
IL-10 receptor and coreceptor expression in quiescent and activated hepatic stellate cells.
Authors: Mathurin P, Xiong S, Kharbanda KK, Veal N, Miyahara T, Motomura K, Rippe RA, Bachem MG, Tsukamoto H
The prognosis of hepatocellular carcinoma (HCC) remains unsatisfactory due to limited effective treatment options. In this work, we investigated the therapeutic efficacy of Terbinafine for HCC and the, 2002-06-01;282(6):G981-90.
Species: Rat
Sample Types: Whole Cells
Applications: ICC