Mouse IL-10 R alpha Antibody

Catalog # Availability Size / Price Qty
AF-474-NA
AF-474-SP
Cell Proliferation Induced by IL‑10 and Neutralization by Mouse IL‑10 R alpha  Antibody.
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Product Details
Citations (7)
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Mouse IL-10 R alpha Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-10 R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 25% cross-reactivity with recombinant human IL-10 R alpha is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑10 R alpha
Thr24-Asn238 (Lys188Arg and Glu222Gly)
Accession # Q61727
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑10 R alpha (Catalog # 474-MR)
Neutralization
Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line [Thompson-Snipes, L.  et al. (1991) J. Exp. Med. 173:507]. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 1 ng/mL Recombinant Mouse IL‑10.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Proliferation Induced by IL‑10 and Neutralization by Mouse IL‑10 R alpha  Antibody. View Larger

Cell Proliferation Induced by IL‑10 and Neutralization by Mouse IL‑10 R alpha Antibody. Recombinant Mouse IL‑10 (Catalog # 417-ML) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑10 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑10 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-474-NA). The ND50 is typically 1-3 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10 R alpha

IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), is a potent immunosuppressant of macrophage functions. IL-10 is also a pleiotropic cytokine with multiple immunostimulatory as well as immunosuppressive effects on a variety of other cell types. IL-10 binds specifically and with high affinity to cell-surface receptors. Mouse and human cDNA clones encoding the ligand-binding IL-10 receptor (IL-10 R) have been isolated. The IL-10 R mRNA has been detected in all cell types that are known to respond to IL-10.

Human and mouse IL-10 receptors are structurally related to the IFN-gamma receptor. These receptors are members of the class II subgroup of the cytokine receptor superfamily. The deduced amino acid sequence of human IL-10 R is approximately 60% identical to mouse IL-10 R. Although human IL-10 has cross-species activities and is active on mouse cells, mouse IL-10 is species-specific in its actions and does not bind to the human IL-10 receptor. The human IL-10 R gene has been mapped to chromosome 11q23.3. Recombinant IL-10 soluble receptor, consisting of the extracellular domain of IL-10 R, binds IL-10 with high affinity in solution and is a potent IL-10 antagonist.

Long Name
Interleukin 10 Receptor alpha
Entrez Gene IDs
3587 (Human); 16154 (Mouse); 102127536 (Cynomolgus Monkey)
Alternate Names
CD210 antigen; CDw210a; HIL-10R; IBD28; IL-10 R alpha; IL-10 receptor subunit alpha; IL10R alpha; IL-10R subunit 1; IL-10R1; IL10RA; IL-10Ra; IL10RIL-10R subunit alpha; interleukin 10 receptor, alpha; interleukin-10 receptor alpha chain; Interleukin-10 receptor subunit 1; interleukin-10 receptor subunit alpha

Product Datasheets

Citations for Mouse IL-10 R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Re-evaluation of IL-10 signaling reveals novel insights on the contribution of the intracellular domain of the IL-10R2 chain
    Authors: RHP Wilbers, DR van Raaij, LB Westerhof, J Bakker, G Smant, A Schots
    PLoS ONE, 2017;12(10):e0186317.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  2. Alternative activation-skewed microglia/macrophages promote hematoma resolution in experimental intracerebral hemorrhage
    Authors: CF Chang, J Wan, Q Li, SC Renfroe, NM Heller, J Wang
    Neurobiol. Dis., 2017;103(0):54-69.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Functional Assay
  3. The Dendritic Cell Receptor DNGR-1 Promotes the Development of Atherosclerosis in Mice
    Authors: Y Haddad, C Lahoute, M Clément, L Laurans, S Metghalchi, Z Lynda, A Giraud, X Loyer, M Vandestien, J Wain-Hobso, B Esposito, S Potteaux, H Ait-Oufell, A Tedgui, Z Mallat, S Taleb
    Circ. Res., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neut
  4. Mesenchymal Stem Cell-Educated Macrophages Ameliorate LPS-Induced Systemic Response
    Authors: Yaoqin Hu
    Mediators Inflamm, 2016;2016(0):3735452.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neut
  5. IL4I1 Is a Novel Regulator of M2 Macrophage Polarization That Can Inhibit T Cell Activation via L-Tryptophan and Arginine Depletion and IL-10 Production.
    Authors: Yue Y, Huang W, Liang J, Guo J, Ji J, Yao Y, Zheng M, Cai Z, Lu L, Wang J
    PLoS ONE, 2015;10(11):e0142979.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neut
  6. Neuronal synapse formation induced by microglia and interleukin 10.
    Authors: Lim S, Park E, You B, Jung Y, Park A, Park S, Lee J
    PLoS ONE, 2013;8(11):e81218.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neut
  7. IL-10 receptor and coreceptor expression in quiescent and activated hepatic stellate cells.
    Authors: Mathurin P, Xiong S, Kharbanda KK, Veal N, Miyahara T, Motomura K, Rippe RA, Bachem MG, Tsukamoto H
    Am. J. Physiol. Gastrointest. Liver Physiol., 2002;282(6):G981-90.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC

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