Mouse IL-17RA/IL-17R Antibody

Catalog # Availability Size / Price Qty
AF448
AF448-SP

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Detection of Mouse IL‑17 RA/IL‑17 R by Western Blot.
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Citations (8)
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Mouse IL-17RA/IL-17R Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL‑17 RA/IL‑17 R in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human IL‑17 RA/IL‑17 R is observed and less than 1% cross-reactivity with recombinant mouse (rm) IL-17 RC and rmIL-17 RD is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑17 RA/IL‑17 R
Extracellular domain
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse IL-17 RA/IL-17 R antibody by Western Blot. View Larger

Detection of Mouse IL‑17 RA/IL‑17 R by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse IL-17 RA/IL-17 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF448) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-17 RA/IL-17 R at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of IL-17 RA/IL-17 R antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of IL‑17 RA/IL‑17 R in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macro-phage cell line was stained with Goat Anti-Mouse IL-17 RA/IL-17 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF448, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17RA/IL-17R

IL-17 R, also known as IL-17 RA, is a 120 kDa type I transmembrane glycoprotein protein that plays a central role in inflammatory responses (1-3). Mature mouse
IL‑17 R consists of a 291 amino acid (aa) extracellular domain, a 21 aa transmembrane segment, and a 521 aa cytoplasmic domain (4). The cytoplasmic domain contains a region homologous to the TIR domain of the TLR/IL-1 R family (5). Mouse IL-17 R shares 84% and 72% aa sequence identity with rat and human IL-17 R, respectively. Within the extracellular domain, it shares 18-25% sequence identity with mouse IL-17 RB, C, D, and E. While the expression of IL-17 is restricted to activated T cells, IL-17 R exhibits a broad tissue distribution (4). Even in the absence of ligand, IL-17 R exists on the cell surface as a multimer (6). IL-17 R can bind IL-17 but must associate with IL-17 RC to transduce signals (7, 8). Interestingly, human IL-17 R does not appear to form productive complexes with mouse IL-17 RC (8). The IL-17 R can also signal in response to IL-17F (9). IL-17 R ligation promotes T cell activation and the production of IL-6, G-CSF, SCF, and multiple pro‑inflammatory chemokines (4, 7, 9, 10). IL-17A and IL-17F synergize with TNF-alpha in the induction of CXCL1, G-CSF, and IL-6 (9, 11). This effect requires the presence of both TNF RI and TNF RII (9). IL-17 interactions with IL-17 R also inhibit the TNF-alpha induced upregulation of fibroblast CCL5 and VCAM-1 (11). CCL5 and VCAM-1 induced effects are differentially sensitive to blockade with IL-17 R specific antibodies, suggesting that IL-17 R triggers divergent intracellular signals (11). In vivo, IL‑17 R activity is important for increased generation of neutrophils and their recruitment to sites of inflammation (10, 12, 13). IL-17 R is required for host defense against microbial infection and for the progression of arthritis from inflammation to destructive joint erosion (10, 13).

References
  1. Iwakura, Y. and H. Ishigame (2006) J. Clin. Invest. 116:1218.
  2. Moseley, T.A. et al. (2003) Cytokine Growth Factor Rev. 14:155.
  3. Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
  4. Yao, Z. et al. (1995) Immunity 3:811.
  5. Novatchkova, M. et al. (2003) Trends Biochem. Sci. 28:226.
  6. Kramer, J.M. et al. (2006) J. Immunol. 176:711.
  7. Hymowitz, S.G. et al. (2001) EMBO J. 20:5332.
  8. Toy, D. et al. (2006) J. Immunol. 177:36.
  9. McAllister, F. et al. (2005) J. Immunol. 175:404.
  10. Ye, P. et al. (2001) J. Exp. Med. 194:519.
  11. Schnyder, B. et al. (2005) Cytokine 31:191.
  12. Tan, W. et al. (2006) J. Immunol. 176:6186.
  13. Lubberts, E. et al. (2005) J. Immunol. 175:3360.
Long Name
Interleukin 17 Receptor
Entrez Gene IDs
23765 (Human); 16172 (Mouse); 312679 (Rat); 486759 (Canine); 101925679 (Cynomolgus Monkey)
Alternate Names
CD217 antigen; CD217; Cdw217; CDw217interleukin 17 receptor; hIL-17R; IL-17 R; IL-17 RA; IL-17 receptor A; IL17RA; IL-17RA; IL-17RAMGC10262; IL17Rinterleukin-17 receptor A; interleukin 17 receptor A

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Citations for Mouse IL-17RA/IL-17R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Population snapshots predict early haematopoietic and erythroid hierarchies
    Authors: BK Tusi, SL Wolock, C Weinreb, Y Hwang, D Hidalgo, R Zilionis, A Waisman, JR Huh, AM Klein, M Socolovsky
    Nature, 2018-02-21;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. IL-17 Augments B Cell Activation in Ocular Surface Autoimmunity
    J Immunol, 2016-09-21;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. IFN-alpha enhances peptide vaccine-induced CD8+ T cell numbers, effector function, and antitumor activity.
    Authors: Sikora AG, Jaffarzad N, Hailemichael Y, Gelbard A, Stonier SW, Schluns KS, Frasca L, Lou Y, Liu C, Andersson HA, Hwu P, Overwijk WW
    J. Immunol., 2009-06-15;182(12):7398-407.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. TLR2 deficiency leads to increased Th17 infiltrates in experimental brain abscesses.
    Authors: Nichols JR, Aldrich AL, Mariani MM, Vidlak D, Esen N, Kielian T
    J. Immunol., 2009-06-01;182(11):7119-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Functional interleukin-17 receptor A is expressed in central nervous system glia and upregulated in experimental autoimmune encephalomyelitis.
    Authors: Das Sarma J, Ciric B, Marek R, Sadhukhan S, Caruso M, Shafagh J, Fitzgerald D, Shindler K, Rostami A
    J Neuroinflammation, 2009-04-28;6(0):14.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. IL-17RD (Sef or IL-17RLM) interacts with IL-17 receptor and mediates IL-17 signaling.
    Authors: Rong Z, Wang A, Li Z, Ren Y, Cheng L, Li Y, Wang Y, Ren F, Zhang X, Hu J, Chang Z
    Cell Res., 2009-02-01;19(2):208-15.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  7. IL-17 receptor signaling influences virus-induced corneal inflammation.
    Authors: Molesworth-Kenyon SJ, Yin R, Oakes JE, Lausch RN
    J. Leukoc. Biol., 2007-11-12;83(2):401-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  8. Inhibition of interleukin-17 prevents the development of arthritis in vaccinated mice challenged with Borrelia burgdorferi.
    Authors: Burchill MA, Nardelli DT, England DM, DeCoster DJ, Christopherson JA, Callister SM, Schell RF
    Infect. Immun., 2003-06-01;71(6):3437-42.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody

WB,ELISA

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