IL‑1 beta /IL‑1F2 in Mouse Splenocytes.|
IL‑1 beta /IL‑1F2 was detected in immersion fixed mouse splenocytes treated with LPS using Recombinant Rat Anti-Mouse IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB401R) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Cell Proliferation Induced by IL-1 beta /IL-1F2 and Neutralization by Mouse|
IL-1 beta /IL-1F2 Antibody. Recombinant Mouse
IL-1 beta /IL-1F2 (Catalog # 401-ML) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse
IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of the Recombinant Rat Anti-Mouse
IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB401R). The ND50 is typically 1-5 μg/mL in the presence of concanavalin A (1.25 μg/mL).
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The mouse IL-1 beta cDNA encodes a 269 aa precursor. A 117 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 65-78% identity with canine, equine, feline, human, porcine, and rhesus macaque IL‑1 beta.
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