Mouse IL-27 p28/IL-30 Antibody AF1834: R&D Systems

Mouse IL-27 p28/IL-30 Antibody

(11 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-27 p28/IL-30 in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse IL-27 p28/IL-30
    Phe29-Ser234
    Accession # Q8K3I6
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse IL‑27 p28/IL-30
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize IL‑27 inhibition of EMCV-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line. Bender, H. et al. (2009) Hepatology 50:585. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑27.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
IL‑27 Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IL‑27 p28/IL‑30 Antibody. Recombinant Mouse IL‑27 (Catalog # 2799-ML) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line), as measured by Resazurin. Inhibition of EMCV activity elicited by Recombinant Mouse IL‑27 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑27 p28/IL-30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1834). The ND50 is typically 1‑5 µg/mL.
Immunohistochemistry
IL‑27 p28/IL‑30 in Mouse Thymus. IL‑27 p28/IL‑30 was detected in perfusion fixed paraffin-embedded sections of mouse thymus using 15 µg/mL Mouse IL‑27 p28/
IL‑30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1834) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-27 p28/IL-30
 IL-27 p28 subunit (also known as IL-30 and Interleukin-27 subunit alpha) is a 26 kDa glycoprotein. IL-27 is a heterodimeric cytokine consisting of the IL-27 p28 subunit that is an IL-12 p35 related protein and EBI3, which is related to IL-12 p40. The biological effects of IL-27 are mediated by the heteromeric receptor complex gp130 and TCCR/WSX-1.
  • Long Name:
    Interleukin 27 p28
  • Entrez Gene IDs:
    246778 (Human); 246779 (Mouse)
  • Alternate Names:
    IL27 p28; IL-27 p28; IL-30
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Thymic DCs derived IL-27 regulates the final maturation of CD4(+) SP thymocytes
    Sci Rep, 2016;6(0):30448.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  2. Type I interferons and microbial metabolites of tryptophan modulate astrocyte activity and central nervous system inflammation via the aryl hydrocarbon receptor
    Nat Med, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  3. Trypanosoma cruzi Infection Imparts a Regulatory Program in Dendritic Cells and T Cells via Galectin-1-Dependent Mechanisms.
    Authors: Poncini C, Ilarregui J, Batalla E, Engels S, Cerliani J, Cucher M, van Kooyk Y, Gonzalez-Cappa S, Rabinovich G
    J Immunol, 2015;195(7):3311-24.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  4. Attenuation of intestinal inflammation in interleukin-10-deficient mice infected with Citrobacter rodentium.
    Authors: Dann, Sara M, Le, Christin, Choudhury, Barun K, Liu, Houpu, Saldarriaga, Omar, Hanson, Elaine M, Cong, Yingzi, Eckmann, Lars
    Infect Immun, 2014;82(5):1949-58.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  5. IL-10-Producing Th1 Cells and Disease Progression Are Regulated by Distinct CD11c+ Cell Populations during Visceral Leishmaniasis.
    Authors: Owens BM, Beattie L, Moore JW, Brown N, Mann JL, Dalton JE, Maroof A, Kaye PM
    PLoS Pathog., 2012;8(7):e1002827.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  6. Human prostate tumor antigen-specific CD8+ regulatory T cells are inhibited by CTLA-4 or IL-35 blockade.
    Authors: Olson B, Jankowska-Gan E, Becker J, Vignali D, Burlingham W, McNeel D
    J Immunol, 2012;189(12):5590-601.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  7. TLR2-mediated production of IL-27 and chemokines by respiratory epithelial cells promotes bleomycin-induced pulmonary fibrosis in mice.
    Authors: Kim HS, Go H, Akira S, Chung DH
    J. Immunol., 2011;187(8):4007-17.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  8. Tolerogenic signals delivered by dendritic cells to T cells through a galectin-1-driven immunoregulatory circuit involving interleukin 27 and interleukin 10.
    Authors: Ilarregui JM, Croci DO, Bianco GA, Toscano MA, Salatino M, Vermeulen ME, Geffner JR, Rabinovich GA
    Nat. Immunol., 2009;10(9):981-91.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  9. The type I IFN induction pathway constrains Th17-mediated autoimmune inflammation in mice.
    Authors: Guo B, Chang EY, Cheng G
    J. Clin. Invest., 2008;118(5):1680-90.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  10. Engagement of the type I interferon receptor on dendritic cells inhibits T helper 17 cell development: role of intracellular osteopontin.
    Authors: Shinohara ML, Kim JH, Garcia VA, Cantor H
    Immunity, 2008;29(1):68-78.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  11. Interleukin-23 and interleukin-27 exert quite different antitumor and vaccine effects on poorly immunogenic melanoma.
    Authors: Oniki S, Nagai H, Horikawa T, Furukawa J, Belladonna ML, Yoshimoto T, Hara I, Nishigori C
    Cancer Res., 2006;66(12):6395-404.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
Expand to show all 11 Citations
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