Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Mouse IL‑27 p28/IL-30
Measured by its ability to neutralize IL‑27 inhibition of EMCV-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line. Bender, H. et al. (2009) Hepatology 50:585. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑27.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
IL‑27 Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IL‑27 p28/IL‑30 Antibody. Recombinant Mouse IL‑27 (Catalog # 2799-ML) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line), as measured by Resazurin. Inhibition of EMCV activity elicited by Recombinant Mouse IL‑27 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑27 p28/IL-30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1834). The ND50 is typically 1‑5 µg/mL.
IL‑27 p28/IL‑30 in Mouse Thymus. IL‑27 p28/IL‑30 was detected in perfusion fixed paraffin-embedded sections of mouse thymus using 15 µg/mL Mouse IL‑27 p28/ IL‑30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1834) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-27 p28/IL-30
IL-27 p28 subunit (also known as IL-30 and Interleukin-27 subunit alpha) is a 26 kDa glycoprotein. IL-27 is a heterodimeric cytokine consisting of the IL-27 p28
subunit that is an IL-12 p35 related protein and EBI3, which is related
to IL-12 p40. The biological effects of IL-27 are mediated by the
heteromeric receptor complex gp130 and TCCR/WSX-1.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.