Mouse IL-33 DuoSet ELISA

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Mouse IL-33 ELISA Standard Curve
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Product Details
Citations (31)
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Reviews (2)

Mouse IL-33 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For five or fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse IL-33. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Data Example

Mouse IL-33 ELISA Standard Curve

Product Datasheets

Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-33

IL-33 (Interleukin-33) is released from physically damaged or necrotic cells. It triggers Th2-biased immune cell activation at sites of inflammation as well as regulatory T cell and M2 macrophage expansion. It additionally induces angiogenesis, promotes tumor cell migration and invasion, limits cardiac myocyte hypertrophy, and limits the development of atherosclerotic plaques. IL-33 signals through a receptor complex composed of ST2 and IL-1 RAcP. IL-1 RAcP also associates with IL-1 RI, IL-1 RII, IL-1 R6, and SCF R/c-kit. Soluble isoforms of ST2 and IL-1 RAcP function as decoy receptors that regulate IL-33 bioactivity.

Long Name:
Interleukin 33
Entrez Gene IDs:
90865 (Human); 77125 (Mouse)
Alternate Names:
C9orf26; C9orf26chromosome 9 open reading frame 26 (NF-HEV); DKFZp586H0523; DVS27; DVS27-related protein; IL1F11; IL-1F11; IL33; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin-33; NFHEV; NF-HEV; NF-HEVNFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2

Assay Procedure


Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Mouse IL-33 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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  1. TET1 contributes to allergic airway inflammation and regulates interferon and aryl hydrocarbon receptor signaling pathways in bronchial epithelial cells
    Authors: JD Burleson, D Siniard, VK Yadagiri, X Chen, MT Weirauch, BP Ruff, EB Brandt, GKK Hershey, H Ji
    Sci Rep, 2019;9(1):7361.
    Species: Mouse
    Sample Types: Tissue Homogenates
  2. Conjugated bile acids attenuate allergen-induced airway inflammation and hyperresposiveness by inhibiting UPR transducers
    Authors: EM Nakada, NR Bhakta, BR Korwin-Mih, A Kumar, N Chamberlai, SR Bruno, DG Chapman, SM Hoffman, N Daphtary, M Aliyeva, CG Irvin, AE Dixon, PG Woodruff, S Amin, ME Poynter, DH Desai, V Anathy
    JCI Insight, 2019;4(9):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  3. Roles for T/B lymphocytes and ILC2s in experimental chronic obstructive pulmonary disease
    Authors: C Donovan, MR Starkey, RY Kim, BMJ Rana, JL Barlow, B Jones, TJ Haw, P Mono Nair, K Budden, GJM Cameron, JC Horvat, PA Wark, PS Foster, ANJ McKenzie, PM Hansbro
    J. Leukoc. Biol., 2018;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  4. Toll-Interacting Protein, Tollip, Inhibits IL-13-Mediated Pulmonary Eosinophilic Inflammation in Mice
    Authors: Y Ito, N Schaefer, A Sanchez, D Francisco, R Alam, RJ Martin, JG Ledford, C Stevenson, D Jiang, L Li, M Kraft, HW Chu
    J Innate Immun, 2018;0(0):.
    Species: Mouse
    Sample Types: BALF
  5. T cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells
    Authors: HR Kim, Y Mun, KS Lee, YJ Park, JS Park, JH Park, BN Jeon, CH Kim, Y Jun, YM Hyun, M Kim, SM Lee, CS Park, SH Im, CD Jun
    Nat Commun, 2018;9(1):3630.
    Species: Mouse
    Sample Types: Cell Lysates
  6. IL-33/ST2 axis mediates hyperplasia of intrarenal urothelium in obstructive renal injury
    Authors: WY Chen, JL Yang, YH Wu, LC Li, RF Li, YT Chang, LH Dai, WC Wang, YJ Chang
    Exp. Mol. Med., 2018;50(4):36.
    Species: Mouse
    Sample Types: Urine
  7. Pulmonary ORMDL3 is critical for induction of Alternaria-induced allergic airways disease
    Authors: S Löser, LG Gregory, Y Zhang, K Schaefer, SA Walker, J Buckley, L Denney, CH Dean, WOC Cookson, MF Moffatt, CM Lloyd
    J. Allergy Clin. Immunol., 2017;139(5):1496-1507.e3.
    Species: Mouse
    Sample Types: Tissue Homogenates
  8. STAT1 Represses Cytokine-Producing Group 2 and Group 3 Innate Lymphoid Cells during Viral Infection
    Authors: MT Stier, K Goleniewsk, JY Cephus, DC Newcomb, TP Sherrill, KL Boyd, MH Bloodworth, ML Moore, K Chen, JK Kolls, RS Peebles
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  9. Behavioral Changes in Mice Lacking Interleukin-33
    Authors: E Dohi, EY Choi, IVL Rose, AS Murata, S Chow, M Niwa, SI Kano
    eNeuro, 2017;4(6):.
    Species: Mouse
    Sample Types: Serum
  10. Experimental atopic dermatitis depends on IL-33R signaling via MyD88 in dendritic cells
    Authors: C Li, I Maillet, C Mackowiak, C Viala, F Di Padova, M Li, D Togbe, V Quesniaux, Y Lai, B Ryffel
    Cell Death Dis, 2017;8(4):e2735.
    Species: Mouse
    Sample Types: Tissue Homogenates
  11. Determinants of Divergent Adaptive Immune Responses after Airway Sensitization with Ligands of Toll-Like Receptor 5 or Toll-Like Receptor 9
    PLoS ONE, 2016;11(12):e0167693.
    Species: Mouse
    Sample Types: Tissue Homogenates
  12. Perinatal Activation of the Interleukin-33 Pathway Promotes Type 2 Immunity in the Developing Lung
    Authors: Ismé M de Kleer
    Immunity, 2016;45(6):1285-1298.
    Species: Mouse
    Sample Types: Tissue Homogenates
  13. Fat-associated lymphoid clusters control local IgM secretion during pleural infection and lung inflammation
    Nat Commun, 2016;7(0):12651.
    Species: Rat
    Sample Types: Pleural Lavage Fluid
  14. Interleukin-33 and RANK-L Interplay in the Alveolar Bone Loss Associated to Periodontitis
    PLoS ONE, 2016;11(12):e0168080.
    Species: Mouse
    Sample Types: Serum
  15. DUOX1 mediates persistent epithelial EGFR activation, mucous cell metaplasia, and airway remodeling during allergic asthma
    JCI Insight, 2016;1(18):e88811.
    Species: Mouse
    Sample Types: BALF
  16. Differential Immunometabolic Phenotype in Th1 and Th2 Dominant Mouse Strains in Response to High-Fat Feeding.
    Authors: Jovicic N, Jeftic I, Jovanovic I, Radosavljevic G, Arsenijevic N, Lukic M, Pejnovic N
    PLoS ONE, 2015;10(7):e0134089.
    Species: Mouse
    Sample Types: Tissue Homogenates
  17. Critical role of IL-33 receptor ST2 in experimental cerebral malaria development.
    Authors: Palomo J, Reverchon F, Piotet J, Besnard A, Couturier-Maillard A, Maillet I, Tefit M, Erard F, Mazier D, Ryffel B, Quesniaux V
    Eur J Immunol, 2015;45(5):1354-65.
    Species: Mouse
    Sample Types: Tissue Homogenates
  18. Dietary galacto-oligosaccharides prevent airway eosinophilia and hyperresponsiveness in a murine house dust mite-induced asthma model.
    Authors: Verheijden K, Willemsen L, Braber S, Leusink-Muis T, Delsing D, Garssen J, Kraneveld A, Folkerts G
    Respir Res, 2015;16(0):17.
    Species: Mouse
    Sample Types: Tissue Homogenates
  19. Type II cytokines impair host defense against an intracellular fungal pathogen by amplifying macrophage generation of IL-33.
    Authors: Verma, A, Kroetz, D N, Tweedle, J L, Deepe, G S Jr
    Mucosal Immunol, 2015;8(2):380-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
  20. The Inflammatory Caspases-1 and -11 Mediate the Pathogenesis of Dermatitis in Sharpin-Deficient Mice.
    Authors: Douglas T, Champagne C, Morizot A, Lapointe J, Saleh M
    J Immunol, 2015;195(5):2365-73.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  21. Mast cell chymase degrades the alarmins heat shock protein 70, biglycan, HMGB1, and interleukin-33 (IL-33) and limits danger-induced inflammation.
    Authors: Roy A, Ganesh G, Sippola H, Bolin S, Sawesi O, Dagalv A, Schlenner S, Feyerabend T, Rodewald H, Kjellen L, Hellman L, Abrink M
    J Biol Chem, 2014;289(1):237-50.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  22. IRF-3, IRF-7, and IPS-1 promote host defense against acute human metapneumovirus infection in neonatal mice.
    Authors: Spann K, Loh Z, Lynch J, Ullah A, Zhang V, Baturcam E, Werder R, Khajornjiraphan N, Rudd P, Loo Y, Suhrbier A, Gale M, Upham J, Phipps S
    Am J Pathol, 2014;184(6):1795-806.
    Species: Mouse
    Sample Types: Tissue Homogenates
  23. Mice deficient in the St3gal3 gene product alpha2,3 sialyltransferase (ST3Gal-III) exhibit enhanced allergic eosinophilic airway inflammation.
    Authors: Kiwamoto T, Brummet M, Wu F, Motari M, Smith D, Schnaar R, Zhu Z, Bochner B
    J Allergy Clin Immunol, 2014;133(1):240-7.e1-3.
    Species: Mouse
    Sample Types: BALF
  24. IL-25 and type 2 innate lymphoid cells induce pulmonary fibrosis.
    Authors: Hams, Emily, Armstrong, Michelle, Barlow, Jillian, Saunders, Sean P, Schwartz, Christia, Cooke, Gordon, Fahy, Ruairi J, Crotty, Thomas B, Hirani, Nikhil, Flynn, Robin J, Voehringer, David, McKenzie, Andrew N, Donnelly, Seamas C, Fallon, Padraic
    Proc Natl Acad Sci U S A, 2014;111(1):367-72.
    Species: Mouse
    Sample Types: Tissue Homogenates
  25. The novel cytokine interleukin-33 activates acinar cell proinflammatory pathways and induces acute pancreatic inflammation in mice.
    Authors: Kempuraj D, Twait E, Williard D, Yuan Z, Meyerholz D, Samuel I
    PLoS ONE, 2013;8(2):e56866.
    Species: Mouse
    Sample Types: Tissue Homogenates
  26. Interleukin-33-dependent innate lymphoid cells mediate hepatic fibrosis.
    Authors: McHedlidze T, Waldner M, Zopf S, Walker J, Rankin A, Schuchmann M, Voehringer D, McKenzie A, Neurath M, Pflanz S, Wirtz S
    Immunity, 2013;39(2):357-71.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  27. Interleukin-1alpha controls allergic sensitization to inhaled house dust mite via the epithelial release of GM-CSF and IL-33.
    J. Exp. Med., 2012;209(8):1505-17.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  28. Control of allergen-induced inflammation and hyperresponsiveness by the metalloproteinase ADAMTS-12.
    Authors: Paulissen G, El Hour M, Rocks N, Gueders M, Bureau F, Foidart J, Lopez-Otin C, Noel A, Cataldo D
    J Immunol, 2012;189(8):4135-43.
    Species: Mouse
    Sample Types: Tissue Homogenates
  29. The Toll-like receptor 5 ligand flagellin promotes asthma by priming allergic responses to indoor allergens.
    Authors: Wilson, Rhonda H, Maruoka, Shuichir, Whitehead, Gregory, Foley, Julie F, Flake, Gordon P, Sever, Michelle, Zeldin, Darryl C, Kraft, Monica, Garantziotis, Stavros, Nakano, Hideki, Cook, Donald N
    Nat Med, 2012;18(11):1705-10.
    Species: Mouse
    Sample Types: BALF
  30. Mast cells as sensors of cell injury through IL-33 recognition.
    Authors: Enoksson M, Lyberg K, Moller-Westerberg C, Fallon PG, Nilsson G, Lunderius-Andersson C
    J. Immunol., 2011;186(4):2523-8.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  31. Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway.
    Authors: Kearley J, Buckland KF, Mathie SA, Lloyd CM
    Am. J. Respir. Crit. Care Med., 2009;179(9):772-81.
    Species: Mouse
    Sample Types: Cell Culture Supernates


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Mouse IL-33 DuoSet ELISA
By Anonymous on 05/06/2017
Sample Tested: Cell culture supernatant

Mouse IL-33 DuoSet ELISA
By Anonymous on 08/03/2016
Sample Tested: bronchial lavage