Mouse Indian Hedgehog/Ihh N-Terminus Antibody

Catalog # Availability Size / Price Qty
AF1705
AF1705-SP
Indian Hedgehog in Mouse Embryo.
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Citations (3)
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Mouse Indian Hedgehog/Ihh N-Terminus Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Indian Hedgehog (Ihh) N-terminal peptide in Western blots. In Western blots, approximately 80% cross-reactivity with recombinant human Shh N-terminal peptide and recombinant mouse (rm) Shh N-terminal peptide is observed, and less than 1% cross‑reactivity with rmIhh C-terminal peptide (amino acids 241-449) is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Indian Hedgehog
Cys28-Gly202
Accession # P97812
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Indian Hedgehog (C28II), N-Terminus
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Indian Hedgehog antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Indian Hedgehog in Mouse Embryo. Indian Hedgehog was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse Indian Hedgehog N-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1705) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Indian Hedgehog/Ihh

The hedgehog (hh) gene encoding a secreted protein was originally identified in Drosophila as a segment polarity gene. The vertebrate homologues of Hh comprise several proteins including sonic hedgehog (Shh), Indian hedgehog (Ihh), and Desert hedgehog (Dhh) (1). Hedgehog proteins are important signaling molecules during embryonic development and are highly conserved within and across species (1). Mouse and human Ihh share 100% amino acid (aa) sequence identity in the signaling domain, while mouse Ihh and Shh share 90% aa identity in the N-terminal signaling domain. Ihh mRNA expression is detected in fetal lung, gut, stomach, liver, kidney, pancreas, and strongly in cartilage, in growth regions of the developing bone (2, 3). Ihh, along with parathyroid hormone related protein, regulate the rate of chondrocyte proliferation and differentiation (4). Ihh is also involved in yolk sac vasculogenesis, playing an important role in differentiation of epiblast cells into endothelial and red blood cells (5).

Mouse Ihh cDNA encodes a 411 aa polypeptide with a predicted 27 aa signal peptide. This polypeptide is cleaved to generate a 45 kDa precursor protein that undergoes the same post-translation processing as Shh (3). An autocatalytic reaction yields a 19 kDa amino-terminal domain Ihh-N protein that retains all known signaling capabilities, and a 23 kDa carboxy-terminal domain Ihh-C protein (3). Since hydrophobic modifications to Shh, including the substitution of the N-terminal cysteine residue with two hydrophobic isoleucine residues, can also increase its potency (6), a similar modification was made for Ihh. This modified form also shows increased potency in a bioassay measuring induction of alkaline phosphatase. At the cell surface, Hedgehog activity is mediated by a multicomponent receptor complex involving the 12-pass transmembrane protein Patched (Ptc) which binds Hedgehogs with high affinity and Smoothened (Smo), a signaling seven transmembrane G-protein coupled receptor (1).

References
  1. Ingham, P. and A. McMahon (2001) Genes & Dev. 15:3059.
  2. Marigo, V. et al. (1995) Genomics 28:44.
  3. Valentini, R.P. et al. (1997) J Biol Chem. 272:8466.
  4. Vortkamp, A. et al. (1996) Science 273:613.
  5. Byrd, N. et al. (2002) Development 129:361.
  6. Taylor, F.R. et al. (2001) Biochemistry 40:4359.
Entrez Gene IDs
3549 (Human); 16147 (Mouse)
Alternate Names
BDA1; BDA1Indian hedgehog homolog (Drosophila); HHG2; HHG-2; Ihh; Indian hedgehog (Drosophila) homolog; Indian hedgehog homolog; indian hedgehog protein; Indian Hedgehog

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Citations for Mouse Indian Hedgehog/Ihh N-Terminus Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Altered IHH signaling contributes to reduced chondrocyte proliferation in the growth plate of MPS VII mice
    Authors: Z Jiang, ALK Derrick-Ro, S Byers
    Mol Genet Metab Rep, 2020-10-22;25(0):100668.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Regulation of Calvarial Osteogenesis by Concomitant De-repression of GLI3 and Activation of IHH Targets
    Authors: LK Veistinen, T Mustonen, MR Hasan, M Takatalo, Y Kobayashi, DA Kesper, A Vortkamp, DP Rice
    Front Physiol, 2017-12-19;8(0):1036.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  3. Inhibition of p38 MAPK signaling in chondrocyte cultures results in enhanced osteogenic differentiation of perichondral cells.
    Authors: Stanton LA, Beier F
    Exp. Cell Res., 2006-10-04;313(1):146-55.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody

WB,ELISA

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