Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Rat, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Laminin alpha 4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Laminin alpha 4
Gln826-Ala1816
Accession # P97927

Specificity

Detects mouse Laminin  alpha 4 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Laminin  alpha 4 Antibody

Laminin a4 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

Laminin alpha 4 in Mouse Embryo.

Laminin a4 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Laminin a4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3837) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to membranes in blood vessels in CNS. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Mouse Laminin alpha 4 by Immunohistochemistry

Detection of Mouse Laminin alpha 4 by Immunohistochemistry

Laminin-alpha 4 staining provides a clear indication of the BBB (A), however pixel intensity varies considerably. Intellesis is able to recognise high and low intensity pixels accurately (B), unlike classical intensity thresholds which result in either too much (C) or too little (D) capillary segmentation. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33935625), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Laminin alpha 4 by Immunohistochemistry

Detection of Mouse Laminin alpha 4 by Immunohistochemistry

Laminin-alpha 4 staining provides a clear indication of the BBB (A), however pixel intensity varies considerably. Intellesis is able to recognise high and low intensity pixels accurately (B), unlike classical intensity thresholds which result in either too much (C) or too little (D) capillary segmentation. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33935625), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Laminin alpha 4 by Immunohistochemistry

Detection of Mouse Laminin alpha 4 by Immunohistochemistry

Laminin-alpha 4 staining provides a clear indication of the BBB (A), however pixel intensity varies considerably. Intellesis is able to recognise high and low intensity pixels accurately (B), unlike classical intensity thresholds which result in either too much (C) or too little (D) capillary segmentation. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33935625), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Laminin  alpha 4 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (13 d.p.c.)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Laminin  alpha 4

Reviewed Applications

Read 2 reviews rated 3.5 using AF3837 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Laminin alpha 4

Laminins are heterotrimeric glycoproteins and are major components of the basement membrane. Each laminin is comprised of a single alpha, beta, and gamma chain that heterotrimerize via their coiled-coil domains to form a large cruciform-shaped molecule. Mature Laminin  alpha 4 is a subunit of Laminin 411, Laminin 421, and Laminin 423. Mature Laminin alpha 4 is a 1792 amino acid (aa) residue protein that has a Laminin N-terminal domain, four Laminin EGF-like domains, the coiled-coil domain, and five C‑terminal tandem Laminin G-like domains. The G-like domains contain binding sites for integrin, heparin, and dystroglycan. Within the region used as immunogen, mouse Laminin  alpha 4 shows 91% and 97% aa sequence identity with human and rat Laminin  alpha 4, respectively.

Alternate Names

LAMA4

Entrez Gene IDs

3910 (Human); 16775 (Mouse); 309816 (Rat)

Gene Symbol

LAMA4

UniProt

P97927

Additional Laminin alpha 4 Products

Product Documents for Mouse Laminin  alpha 4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Laminin  alpha 4 Antibody

For research use only

Related Research Areas

Extracellular Matrix Molecules

Citations for Mouse Laminin  alpha 4 Antibody

Customer Reviews for Mouse Laminin  alpha 4 Antibody (2)

3.5 out of 5
2 Customer Ratings
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Customer Images

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  • Mouse Laminin  alpha 4 Antibody
    Name: Bhavin Shah
    Application: Immunoflourescence in Frozen mouse brain sections
    Sample Tested: P12 20µm mouse brain frozen sections
    Species: Mouse
    Verified Customer | Posted 11/25/2021
    Immunostaining of the blood vessels in P12 mice brain sections using Laminin a4 antibody.
    A lot of standardisation may be required for the antibody to work. good fixation of the tissue may help. for adult tissues, they need to be perfused well in order to achieve good results.
    Mouse Laminin  alpha 4 Antibody AF3837
  • Mouse Laminin  alpha 4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Brain tissue
    Species: Mouse
    Verified Customer | Posted 07/16/2019
    Dilution 1:400
    Mouse Laminin  alpha 4 Antibody AF3837

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Protocols

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