Mouse Lymphotoxin-alpha /TNF-beta Antibody

Catalog # Availability Size / Price Qty
AF749
AF749-SP
Product Details
Citations (1)
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Mouse Lymphotoxin-alpha /TNF-beta Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Lymphotoxin‑ alpha /TNF‑ beta in direct ELISAs and Western blots. In direct ELISAs, 100% cross‑reactivity with recombinant human Lymphotoxin‑ alpha /TNF‑ beta is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Lymphotoxin‑ alpha /TNF‑ beta
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Lymphotoxin‑ alpha /TNF‑ beta

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lymphotoxin-alpha/TNF-beta

Tumor necrosis factor-beta (TNF-beta ), also known as lymphotoxin-alpha (LT-alpha ), is a secreted homotrimeric glycoprotein belonging to the TNF superfamily and is designated TNFSF1B. It is produced by NK, T, and B cells. TNF-beta was originally identified as protein that kills tumor cells in cell culture supernatants of a lymphoblastoid cell line. The TNF-beta subunit also associates with the type II transmembrane TNF superfamily protein lymphotoxin beta (LT beta ) to generate two types of heterotrimers designated as LT alpha 1 beta 2 (a single TNF-beta chain non-covalently associated with two chains of LT beta ), and LT alpha 2 beta 1 (1, 2). TNF-alpha, TNF-beta, and LT beta form a subfamily of the TNF related ligands. Their genes are genetically linked within a compact cluster inside the major histocompatibility complex locus (2, 3). The soluble TNF-beta binds and signals through TNF R1 and TNF R2. In contrast, the membrane-bound LT alpha 1 beta 2 interacts specifically with the LT beta receptor (LT beta R), which does not bind TNF-beta or TNF-alpha. Both TNFR1 and TNFR2 bind LT alpha 2 beta 1, which is recognized weakly by LT beta R (4, 5). TNF R1 and 2 express very broadly, while expression of LT beta  R is restricted to stromal cells of lymphoid tissues. Herpesvirus entry mediator binds TNF-beta in vitro (6). The physiological importance of such interaction, if it occurs in vivo, is unclear. Distinct functions attributed to TNF-beta from transgenic knock-out mice include, loss of lymph node development, change in splenic architecture, impaired germinal center formation, and susceptibility to pulmonary tuberculosis (7, 8). TNF-beta also has overlapping physiological functions with LT beta and TNF-alpha in lymphoid organogenesis (7). Mouse and human TNF-beta share approximately 74% homology in their amino acid sequence.

References
  1. Aggarwal, B.B. (2003) Nature Rev. Immunol. 3:745.
  2. Browning, J.L. et al. (1993) Cell 72:846.
  3. Browning, J.L. et al. (1995) J. Immunol. 154:33.
  4. Pokholok, D.K. et al. (1995) Proc. Natl. Acad. Sci. USA 92:674.
  5. Aggarwal, B.B. et al. (1985) Nature 318:665.
  6. Crowe, P.D. et al. (1994) Science 264:707.
  7. Mauri, D.N. et al. (1998) Immunity 8:21.
  8. Tumanov, A.V. et al. (2003) Cytokine & Growth Factor Rev. 14:275.
  9. Roach, D.R. et al. (2001) J. Exp. Med. 193:239.
Long Name
Tumor Necrosis Factor beta
Entrez Gene IDs
4049 (Human); 16992 (Mouse)
Alternate Names
LT; LTA; LT-alpha; lymphotoxin alpha (TNF superfamily, member 1); Lymphotoxin alpha; Lymphotoxinalpha; Lymphotoxin-alpha; tnfb; TNF-beta; TNFBlymphotoxin-alpha; TNFSF1; TNFSF1B; TNFSF1TNF-beta; tumor necrosis factor beta; Tumor necrosis factor ligand superfamily member 1

Product Datasheets

Citation for Mouse Lymphotoxin-alpha /TNF-beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Astroglial-derived lymphotoxin-alpha exacerbates inflammation and demyelination, but not remyelination.
    Authors: Plant SR, Arnett HA, Ting JP
    Glia, 2005;49(1):1-14.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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Secondary Antibodies

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