Mouse Lymphotoxin  beta R/TNFRSF3 Antibody

(6 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Lymphotoxin beta R/TNFRSF3 in direct ELISA and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) LT beta R is observed, and less than 1% cross-reactivity with recombinant mouse (rm) 4-1BB, rmCD27, rmOPG, rmRANK, rmCD30, rmCD40, rhDR6, rmTNF RI, rmTNF RII, rmEDAR, rmFas and rmGITR is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Lymphotoxin beta R/TNFRSF3
    Ser28-Pro218
    Accession # P50284
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Lymphotoxin  beta R/TNFRSF3
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunohistochemistry
    5-15 µg/mL
    Perfusion fixed frozen sections of mouse thymus
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Lymphotoxin  beta R/TNFRSF3 in NIH‑3T3 Mouse Cell Line by Flow Cytometry. NIH‑3T3 mouse embryonic fibroblast cell line was stained with Goat Anti-Mouse Lymphotoxin  beta R/TNFRSF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1008, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lymphotoxin beta R/TNFRSF3
Lymphotoxin beta R is a member of the TNF receptor superfamily and is now designated TNFRSF3. Lymphotoxin beta R transduces signals following binding of LIGHT or the heterotrimeric Lymphotoxins LT alpha 1/ beta 2 or LT alpha 2/ beta 1. It plays a critical role in controlling cellular immune functions and lymphoid organogenesis.
  • Long Name:
    Lymphotoxin beta Receptor
  • Entrez Gene IDs:
    4055 (Human); 17000 (Mouse); 297604 (Rat)
  • Alternate Names:
    CD18; D12S370; ltbetar; LT-BETA-R; LTBR; lymphotoxin B receptor; Lymphotoxin beta R; lymphotoxin beta receptor (TNFR superfamily, member 3); Lymphotoxin-beta receptor; LymphotoxinbR; TNF RIII; TNF Rrp; TNFCRTNF-RIII; TNFR superfamily, member 3; TNFR2-RP; TNFR3; TNF-R-III; TNFR-RP; TNFRSF3; TNFRSF3TNFR-III; Tumor necrosis factor C receptor; Tumor necrosis factor receptor 2-related protein; tumor necrosis factor receptor superfamily member 3; tumor necrosis factor receptor superfamily, member 3; Tumor necrosis factor receptor type III
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. A dendritic-cell-stromal axis maintains immune responses in lymph nodes.
    Authors: Kumar V, Dasoveanu D, Chyou S, Tzeng T, Rozo C, Liang Y, Stohl W, Fu Y, Ruddle N, Lu T
    Immunity, 2015;42(4):719-30.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  2. Autotaxin produced by stromal cells promotes LFA-1-independent and Rho-dependent interstitial T cell motility in the lymph node paracortex.
    Authors: Katakai T, Kondo N, Ueda Y, Kinashi T
    J Immunol, 2014;193(2):617-26.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Functional Assay
  3. TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice.
    Authors: Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM
    J. Clin. Invest., 2009;119(10):2954-64.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  4. CXCL13 production by an established lymph node stromal cell line via lymphotoxin-beta receptor engagement involves the cooperation of multiple signaling pathways.
    Authors: Suto H, Katakai T, Sugai M, Kinashi T, Shimizu A
    Int. Immunol., 2009;21(4):467-76.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  5. Organizer-like reticular stromal cell layer common to adult secondary lymphoid organs.
    Authors: Katakai T, Suto H, Sugai M, Gonda H, Togawa A, Suematsu S, Ebisuno Y, Katagiri K, Kinashi T, Shimizu A
    J. Immunol., 2008;181(9):6189-200.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  6. Lymph node fibroblastic reticular cells construct the stromal reticulum via contact with lymphocytes.
    Authors: Katakai T, Hara T, Sugai M, Gonda H, Shimizu A
    J. Exp. Med., 2004;200(6):783-95.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
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