MDL‑1/CLEC5A in RAW 264.7 Mouse Cell Line. MDL‑1/CLEC5A was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Goat Anti-Mouse MDL‑1/CLEC5A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1639) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
MDL-1 is an
approximately 40 kDa transmembrane glycoprotein belonging to the C-type lectin
superfamily (CLEC5A). MDL-1 is expressed on immature myeloid cells, monocytes,
macrophages, dendritic cells, neutrophils, NK cells, and osteocytes. It
contains a charged lysine in the transmembrane region that enables it to
associate with DAP12 and deliver an activating signal. MDL-1 mediates inflammatory responses during
autoimmune arthritis and upon binding to Dengue and Japanese encephalitis
viruses. The extracellular domain (ECD) of MDL-1 contains a juxtamembrane stalk
region and one C-type lectin domain. Within the ECD, mouse and human MDL-1
share 67% amino acid sequence identity. In mouse, alternative splicing
generates a short isoform with a 25 aa deletion in the stalk region.
Myeloid DAP12-associating Lectin
Entrez Gene IDs:
23601 (Human); 23845 (Mouse)
CLEC5A; CLECSF5; CLECSF5carbohydrate-recognition domain) lectin, superfamilymember 5; C-type lectin domain family 5, member A; C-type lectin superfamily member 5; MDL1; MDL-1MGC138304; Myeloid DAP12-associating lectin 1; myeloid DAP12-associating lectin-1
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