Mouse MESDC2 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse MESDC2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with human MESDC2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse MESDC2
    Ala30-Leu224
    Accession # Q9ERE7
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
MESDC2 in Mouse Testis. MESDC2 was detected in perfusion fixed frozen sections of mouse testis using 10 µg/mL Goat Anti-Mouse MESDC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4545) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Detection of Mouse MESDC2 by Western Blot. Western blot shows lysates of mouse brain tissue, NS0 mouse myeloma cell line, and D3 mouse embryonic stem cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse MESDC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4545) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MESDC2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MESDC2

Mesoderm development candidate gene 2 (MESDC2, Mesd), also known as Boca in drosophila, is a 22 kDa protein that is required for formation of the primitive streak and mesoderm during embryogenesis (1-3). Mature mouse MESDC2 consists of an 83 amino acid (aa) structured central domain with flexible N- and C-terminal regions (4, 5). It shares 89% and 96% aa sequence identity with human and rat MESDC2, respectively. Although Boca lacks 34 aa in the C-terminal region and is only 40% identical with mouse MESDC2, the mouse protein can functionally substitute for Boca in drosophila S2 cells (6). A C-terminal ER retention motif localizes MESDC2 and Boca to the lumen of the endoplasmic reticulum (2, 6, 7). Within the ER, MESDC2 binds to the Wnt co-receptors LRP5 and LRP6 and is required for their proper folding and cell surface expression (2, 5, 8, 9). MESDC2 is therefore important for cellular Wnt responsiveness (9). When added extracellularly, MESDC2 binds to cell surface LRP6, preventing its interaction with the Wnt antagonist Dkk-1. This binding does not, however, trigger LRP6 internalization or alteration of cytoplasmic beta -catenin levels (8). An LRP5 mutant associated with high bone mass does not interact with MESDC2 (10). MESDC2 itself can be disrupted by a chromosomal translocation occurring in the germ cell tumor, infantile sacrococcygeal teratoma (11).

  • References:
    1. Holdener, B.C. et al. (1994) Development 120:1335.
    2. Hsieh, J-C. et al. (2003) Cell 112:355.
    3. Kimelman, D. (2006) Nat. Rev. Genet. 7:360.
    4. Wines, M.E. et al. (2001) Genomics 72:88.
    5. Koduri, V. and S.C. Blacklow (2007) Biochemistry 46:6570.
    6. Culi, J. et al. (2004) EMBO J. 23:1372.
    7. Culi, J. and R.S. Mann (2003) Cell 112:343.
    8. Li, Y. et al. (2005) J. Cell Sci. 118:5305.
    9. Li, Y. et al. (2006) FEBS Lett. 580:5423.
    10. Zhang, Y. et al. (2004) Mol. Cell. Biol. 24:4677.
    11. Veltman, I.M. et al. (2005) Hum. Mol. Genet. 14:1955.
  • Long Name:
    Mesoderm Development Candidate 2
  • Entrez Gene IDs:
    23184 (Human)
  • Alternate Names:
    BOCA; KIAA0081Renal carcinoma antigen NY-REN-61; MESDC2; MESDLDLR chaperone MESD; mesoderm development candidate 2Mesoderm development protein
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