Mouse MFG-E8 Antibody Summary
Ala23-Cys463
Accession # P21956
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse MFG‑E8 by Western Blot. Western blot shows lysates of mouse mammary gland tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse MFG-E8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2805) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MFG-E8 at approximately 60-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Mouse MFG‑E8 by Simple WesternTM. Simple Western lane view shows lysates of recombinant mouse (rm) MFG-E8, loaded at 50 ng/mL. A specific band was detected for MFG-E8 at approximately 87 kDa (as indicated) using 50 µg/mL of Goat Anti-Mouse MFG-E8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2805) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MFG-E8
Milk Fat Globulin Protein E8 (MFG-E8), also known as Lactadherin, MP47, breast epithelial antigen BA46, and SED1, is a 66‑75 kDa pleiotropic secreted glycoprotein that promotes mammary gland morphogenesis, angiogenesis, and tumor progression. MFG-E8 also plays an important role in tissue homeostasis and the prevention of inflammation (1). Mouse MGF-E8 contains two N-terminal EGF-like domains, a Pro/Thr-rich segment, and two C-terminal F5/8-type discoidin-like domains (2). It MFG-E8 shares 63% and 94% aa sequence identity with comparable regions of human and rat MFG-E8, respectively. Alternative splicing of mouse MFG-E8 generates a short isoform lacking the Pro/Thr-rich region which contains sites for O-linked glycosylation and tyrosine sulfation (3). MFG-E8 is released into the milk in complex with lipid-containing milk fat globules. It is also found in multiple other cell types including endothelial cells and smooth muscle cells of the vasculature, immature dendritic cells, at the acrosomal cap of testicular and epididymal sperm, and in epithelial cells of the endometrium (1). MFG-E8 binds to the Integrins alpha V beta 3 and alpha V beta 5 and potentiates the angiogenic action of VEGF through VEGF R2 (4, 5). It reduces inflammation and tissue damage in a variety of settings. MFG-E8 functions as a bridge between phosphatidylserine on apoptotic cells and Integrin alpha V beta 3 on phagocytes, leading to the clearance of apoptotic debris (6). It mediates the engulfment of apoptotic bodies in atherosclerotic plaques and prion-infected brain (7, 8) and of apoptotic B cells during germinal center reactions (9, 10). MFG-E8 also promotes the removal of excess Collagen in fibrotic lungs and the regeneration of damaged intestinal epithelia (11, 12). Its tissue-protective role impairs anti‑tumor immunity and chemotherapy-induced apoptosis (13). MFG-E8 in the breastmilk blocks rotavirus infection in nursing babies (14).
- Raymond, A. et al. (2009) J. Cell. Biochem. 106:957.
- Stubbs, J.D. et al. (1990) Proc. Natl. Acad. Sci. USA 87:8417.
- Hoffhines, A.J. et al. (2008) J. Biol. Chem. 284:3096.
- Silvestre, J.-S. et al. (2005) Nat. Med. 11:499.
- Borges, E. et al. (2000) J. Biol. Chem. 275:39867.
- Hanayama, R. et al. (2002) Nature 417:182.
- Ait-Oufella, H. et al. (2007) Circulation 115:2168.
- Kranich, J. et al. (2010) J. Exp. Med. 207:2271.
- Hanayama, R. et al. (2004) Science 304:1147.
- Kranich, J. et al. (2010) J. Exp. Med. 205:1293.
- Atabai, K. et al. (2009) J. Clin. Invest. 119:3713.
- Bu, H.-F. et al. (2007) J. Clin. Invest. 117:3673.
- Jinushi, M. et al. (2009) J. Exp. Med. 206:1317.
- Kvistgaard, A.S. et al. (2004) J. Dairy Sci. 87:4088.
Product Datasheets
Citations for Mouse MFG-E8 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
16
Citations: Showing 1 - 10
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Inter-axonal molecular crosstalk via Lumican proteoglycan sculpts murine cervical corticospinal innervation by distinct subpopulations
Authors: Y Itoh, V Sahni, SJ Shnider, H McKee, JD Macklis
Cell Reports, 2023-03-17;42(3):112182.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Immunoprecipitation -
Small extracellular vesicles from Ptpn1-deficient macrophages alleviate intestinal inflammation by reprogramming macrophage polarization via lactadherin enrichment
Authors: D Han, D Lu, S Huang, J Pang, Y Wu, J Hu, X Zhang, Y Pi, G Zhang, J Wang
Redox Biology, 2022-11-28;58(0):102558.
Species: Transgenic Mouse
Sample Types: In Vivo
Applications: In Vivo -
Medin co-aggregates with vascular amyloid-beta in Alzheimer's disease
Authors: J Wagner, K Degenhardt, M Veit, N Louros, K Konstantou, A Skodras, K Wild, P Liu, U Obermüller, V Bansal, A Dalmia, LM Häsler, M Lambert, M De Vleesch, HA Davies, J Madine, D Kronenberg, R Feederle, D Del Turco, KPR Nilsson, T Lashley, T Deller, M Gearing, LC Walker, P Heutink, F Rousseau, J Schymkowit, M Jucker, JJ Neher
Nature, 2022-11-16;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
The Potential of Bovine Colostrum-Derived Exosomes to Repair Aged and Damaged Skin Cells
Authors: G Han, H Kim, DE Kim, Y Ahn, J Kim, YJ Jang, K Kim, Y Yang, SH Kim
Pharmaceutics, 2022-01-27;14(2):.
Species: Bovine
Sample Types: Milk Exosomes
Applications: Western Blot -
MFG-E8 (LACTADHERIN): a novel marker associated with cerebral amyloid angiopathy
Authors: P Marazuela, M Solé, A Bonaterra-, J Pizarro, J Camacho, E Martínez-S, HB Kuiperij, MM Verbeek, AM de Kort, FHBM Schreuder, CJM Klijn, L Castillo-R, O Pancorbo, D Rodríguez-, F Pujadas, P Delgado, M Hernández-
Acta neuropathologica communications, 2021-09-16;9(1):154.
Species: Mouse, Transgenic Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
MFG-E8 regulated by miR-99b-5p protects against osteoarthritis by targeting chondrocyte senescence and macrophage reprogramming via the NF-&kappaB pathway
Authors: Y Lu, L Liu, J Pan, B Luo, H Zeng, Y Shao, H Zhang, H Guan, D Guo, C Zeng, R Zhang, X Bai, H Zhang, D Cai
Cell Death & Disease, 2021-05-25;12(6):533.
Species: Mouse
Sample Types: Protein
Applications: Western Blot -
MFG-E8 Plays an Important Role in Attenuating Cerulein-Induced Acute Pancreatitis in Mice
Authors: HF Bu, S Subramania, H Geng, X Wang, F Liu, PM Chou, C Du, IG De Plaen, XD Tan
Cells, 2021-03-25;10(4):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Essential omega-3 fatty acids tune microglial phagocytosis of synaptic elements in the mouse developing brain
Authors: C Madore, Q Leyrolle, L Morel, M Rossitto, AD Greenhalgh, JC Delpech, M Martinat, C Bosch-Bouj, J Bourel, B Rani, C Lacabanne, A Thomazeau, KE Hopperton, S Beccari, A Sere, A Aubert, V De Smedt-P, C Lecours, K Bisht, L Fourgeaud, S Gregoire, L Bretillon, N Acar, NJ Grant, J Badaut, P Gressens, A Sierra, O Butovsky, ME Tremblay, RP Bazinet, C Joffre, A Nadjar, S Layé
Nature Communications, 2020-11-30;11(1):6133.
Species: Mouse
Sample Types: Protein
Applications: Western Blot -
Cardiomyocytes capture stem cell-derived, anti-apoptotic microRNA-214 via clathrin-mediated endocytosis in acute myocardial infarction
Authors: S Eguchi, M Takefuji, T Sakaguchi, S Ishihama, Y Mori, T Tsuda, T Takikawa, T Yoshida, K Ohashi, Y Shimizu, R Hayashida, K Kondo, YK Bando, N Ouchi, T Murohara
J. Biol. Chem., 2019-06-19;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Living Neurons with Tau Filaments Aberrantly Expose Phosphatidylserine and Are Phagocytosed by Microglia
Authors: J Brelstaff, AM Tolkovsky, B Ghetti, M Goedert, MG Spillantin
Cell Rep, 2018-08-21;24(8):1939-1948.e4.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Obesity and Insulin Resistance Promote Atherosclerosis through an IFN?-Regulated Macrophage Protein Network
Authors: CA Reardon, A Lingaraju, KQ Schoenfelt, G Zhou, C Cui, H Jacobs-El, I Babenko, A Hoofnagle, D Czyz, H Shuman, T Vaisar, L Becker
Cell Rep, 2018-06-05;23(10):3021-3030.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Adiponectin/T-cadherin system enhances exosome biogenesis and decreases cellular ceramides by exosomal release
Authors: Y Obata, S Kita, Y Koyama, S Fukuda, H Takeda, M Takahashi, Y Fujishima, H Nagao, S Masuda, Y Tanaka, Y Nakamura, H Nishizawa, T Funahashi, B Ranscht, Y Izumi, T Bamba, E Fukusaki, R Hanayama, S Shimada, N Maeda, I Shimomura
JCI Insight, 2018-04-19;3(8):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Anti-HER2 scFv-directed extracellular vesicle-mediated mRNA-based gene delivery inhibits growth of HER2-positive human breast tumor xenografts by prodrug activation
Authors: JH Wang, AV Forterre, J Zhao, DO Frimannsso, A Delcayre, TJ Antes, B Efron, SS Jeffrey, MD Pegram, AC Matin
Mol. Cancer Ther., 2018-02-26;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Developmental Emergence of Adult Neural Stem Cells as Revealed by Single-Cell Transcriptional Profiling
Authors: SA Yuzwa, MJ Borrett, BT Innes, A Voronova, T Ketela, DR Kaplan, GD Bader, FD Miller
Cell Rep, 2017-12-26;21(13):3970-3986.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Spherical nucleic acid targeting microRNA-99b enhances intestinal MFG-E8 gene expression and restores enterocyte migration in lipopolysaccharide-induced septic mice
Sci Rep, 2016-08-19;6(0):31687.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
MFG-E8 regulates microglial phagocytosis of apoptotic neurons.
Authors: Fuller AD, Van Eldik LJ
J Neuroimmune Pharmacol, 2008-08-01;3(4):246-56.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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Immunohistochemistry/fluorescence (Frozen sections).
10 µm cryostat sections from fresh frozen brain sample. Post-fixation with 4% formaldehyde for 15min..
Blocking: PBS + 1% BSA +1% ovalbumin +10% normal donkey serum +0.3% LDAO +0.3M Glycine.
Incubation buffer: PBS + 1% BSA +1% ovalbumin +10% normal donkey serum.
Primary AB: goat anti-mouse MFG-E8 (#AF2805) 2 µg/ml, 2h at room temperature.
DRAQ5 5µM (nuclei marker) together with primary AB.
Secondary AB: Donkey anti-goat/AlexaFluor488 4 µg/ml, 1h at RT.
Autofluorescence suppression: 4mM CuSO4 in 50mM ammoniumacetate buffer, pH 5, 30min at RT.
Imaging: Evident SlideView VS200 slide scanner with X-Cite NOVEM illumination.
Dilution: 1:1000
Secondary used: Goat IgG Horseradish Peroxidase-conjugated Antibody from R&D Systems.
