hh Mouse MFG-E8 Antibody AF2805: R&D Systems

Mouse MFG-E8 Antibody

(2 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse MFG-E8 in direct ELISAs and Western blots. In these formats, less than 2% cross‑reactivity with recombinant human MFG-E8 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse MFG-E8 (R&D Systems, Catalog # 2805-MF)
    Ala23-Cys463
    Accession # P21956
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse MFG‑E8 by Western Blot. Western blot shows lysates of mouse mammary gland tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse MFG‑E8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2805) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MFG‑E8 at approximately 60-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MFG-E8
Milk Fat Globulin Protein E8 (MFG-E8), also known as Lactadherin, MP47, breast epithelial antigen BA46, and SED1, is a 66‑75 kDa pleiotropic secreted glycoprotein that promotes mammary gland morphogenesis, angiogenesis, and tumor progression. MFG-E8 also plays an important role in tissue homeostasis and the prevention of inflammation (1). Mouse MGF-E8 contains two N-terminal EGF-like domains, a Pro/Thr-rich segment, and two C-terminal F5/8-type discoidin-like domains (2). It MFG-E8 shares 63% and 94% aa sequence identity with comparable regions of human and rat MFG-E8, respectively. Alternative splicing of mouse MFG-E8 generates a short isoform lacking the Pro/Thr-rich region which contains sites for O-linked glycosylation and tyrosine sulfation (3). MFG-E8 is released into the milk in complex with lipid-containing milk fat globules. It is also found in multiple other cell types including endothelial cells and smooth muscle cells of the vasculature, immature dendritic cells, at the acrosomal cap of testicular and epididymal sperm, and in epithelial cells of the endometrium (1). MFG-E8 binds to the Integrins alpha V beta 3 and alpha V beta 5 and potentiates the angiogenic action of VEGF through VEGF R2 (4, 5). It reduces inflammation and tissue damage in a variety of settings. MFG-E8 functions as a bridge between phosphatidylserine on apoptotic cells and Integrin alpha V beta 3 on phagocytes, leading to the clearance of apoptotic debris (6). It mediates the engulfment of apoptotic bodies in atherosclerotic plaques and prion-infected brain (7, 8) and of apoptotic B cells during germinal center reactions (9, 10). MFG-E8 also promotes the removal of excess Collagen in fibrotic lungs and the regeneration of damaged intestinal epithelia (11, 12). Its tissue-protective role impairs anti‑tumor immunity and chemotherapy-induced apoptosis (13). MFG-E8 in the breastmilk blocks rotavirus infection in nursing babies (14).
  • References:
    1. Raymond, A. et al. (2009) J. Cell. Biochem. 106:957.
    2. Stubbs, J.D. et al. (1990) Proc. Natl. Acad. Sci. USA 87:8417.
    3. Hoffhines, A.J. et al. (2008) J. Biol. Chem. 284:3096.
    4. Silvestre, J.-S. et al. (2005) Nat. Med. 11:499.
    5. Borges, E. et al. (2000) J. Biol. Chem. 275:39867.
    6. Hanayama, R. et al. (2002) Nature 417:182.
    7. Ait-Oufella, H. et al. (2007) Circulation 115:2168.
    8. Kranich, J. et al. (2010) J. Exp. Med. 207:2271.
    9. Hanayama, R. et al. (2004) Science 304:1147.
    10. Kranich, J. et al. (2010) J. Exp. Med. 205:1293.
    11. Atabai, K. et al. (2009) J. Clin. Invest. 119:3713.
    12. Bu, H.-F. et al. (2007) J. Clin. Invest. 117:3673.
    13. Jinushi, M. et al. (2009) J. Exp. Med. 206:1317.
    14. Kvistgaard, A.S. et al. (2004) J. Dairy Sci. 87:4088.
  • Long Name:
    Milk Fat Globule EGF Factor 8
  • Entrez Gene IDs:
    4240 (Human); 17304 (Mouse)
  • Alternate Names:
    BA46; Breast epithelial antigen BA46; EDIL1; hP47; HsT19888; lactadherin; Lactahedrin; Medin; MFG1; MFGE8; MFG-E8; MFGM; milk fat globule-EGF factor 8 protein; Milk fat globule-EGF factor 8; O-acetyl disialoganglioside synthase; OAcGD3S; SED1; SPAG10; sperm associated antigen 10; sperm surface protein hP47
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Applications
Sample Type
  1. Spherical nucleic acid targeting microRNA-99b enhances intestinal MFG-E8 gene expression and restores enterocyte migration in lipopolysaccharide-induced septic mice
    Sci Rep, 2016;6(0):31687.
  2. MFG-E8 regulates microglial phagocytosis of apoptotic neurons.
    Authors: Fuller AD, Van Eldik LJ,
    J Neuroimmune Pharmacol, 2008;3(4):246-56.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 application: Western Blot.

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Western Blot Mouse MFG-E8 Antibody AF2805
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 WB Porcine Anonymous 09/07/2017
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Western Blot Mouse MFG-E8 Antibody AF2805
Western Blot: Mouse MFG-E8 Antibody [AF2805]

Summary

ApplicationWestern Blot
Sample TestedSperm
SpeciesPorcine

Other Experimental Details

Other Experimental DetailsDilution: 1:1000 Secondary used: Goat IgG Horseradish Peroxidase-conjugated Antibody from R&D Systems.

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