Detection of MICL/CLEC12A in M1 Mouse Cell Line by Flow Cytometry.
M1 mouse myeloid leukemia cell line was stained with Goat Anti-Mouse MICL/CLEC12A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2950, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
MICL/CLEC12A was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse MICL/CLEC12A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2950) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
MICL, also known as killer cell lectin-like receptor 1 (KLR1), is an inhibitory C-type lectin-like receptor that contains an immunoreceptor tyrosine-based inhibitory motif (ITIM). It is a75 kDa type II membrane protein that is expressed on neutrophils, NK cells, monocytes and dendritic cells. The amino acid sequence of mouse MICL extracellular domain is 53%, 52% and 58% identical to that of human, bovine and canine MICL, respectively.
Myeloid Inhibitory C-type Lectin-like Receptor
Entrez Gene IDs:
160364 (Human); 232413 (Mouse)
CD371; CLEC12A; CLL1; CLL-1; C-type lectin domain family 12, member A; DCAL-2; MICL
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