Mouse Nanog Antibody

(15 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects recombinant mouse Nanog in Western blots. In this format, approximately 50% cross-reactivity with recombinant human Nanog is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse Nanog
    Trp154-Leu262
    Accession # Q80Z64
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    Yang, W. et al. (2014) Nat. Comm. 5:3818
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    0.25 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Nanog in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Goat Anti-Mouse Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2729, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
Immunocytochemistry
Nanog in D3 Mouse Cell Line. Nanog was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Mouse Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2729) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nanog
Nanog is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Its expression is high in undifferentiated embryonic stem cells and is down-regulated during embryonic stem cell differentiation, concomitant with loss of pluripotency
(1-3).
  • References:
    1. Mitsui, K. et al. (2003) Cell 11:631.
    2. Chambers, I. et al. (2003) Cell 113:643.
    3. Hart, A.H. et al. (2004) Dev. Dyn. 230:187.
  • Long Name:
    Nanog Homeobox
  • Entrez Gene IDs:
    79923 (Human); 71950 (Mouse)
  • Alternate Names:
    FLJ12581; FLJ40451; hNanog; homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox; Nanog
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017;8(17):28074-28092.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Lysine-specific histone demethylase 1 inhibition promotes reprogramming by facilitating the expression of exogenous transcriptional factors and metabolic switch
    Sci Rep, 2016;6(0):30903.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Fresh
  3. ROCK inhibitor primes human induced pluripotent stem cells to selectively differentiate towards mesendodermal lineage via epithelial-mesenchymal transition-like modulation
    Stem Cell Res, 2016;17(2):222-227.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Not Specified
  4. Acute loss of Cited2 impairs Nanog expression and decreases self-renewal of mouse embryonic stem cells.
    Authors: Kranc K, Oliveira D, Armesilla-Diaz A, Pacheco-Leyva I, Catarina Matias A, Luisa Escapa A, Subramani C, Wheadon H, Trindade M, Nichols J, Kaji K, Enver T, Braganca J
    Stem Cells, 2015;33(3):699-712.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  5. Embryonic Stem Cell Growth Factors Regulate eIF2alpha Phosphorylation.
    Authors: Friend K, Brooks H, Propson N, Thomson J, Kimble J
    PLoS ONE, 2015;10(9):e0139076.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  6. FBXL10 protects Polycomb-bound genes from hypermethylation.
    Authors: Boulard M, Edwards J, Bestor T
    Nat Genet, 2015;47(5):479-85.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Not Specified
  7. Cyclin-dependent kinase-mediated Sox2 phosphorylation enhances the ability of Sox2 to establish the pluripotent state.
    Authors: Ouyang J, Yu W, Liu J, Zhang N, Florens L, Chen J, Liu H, Washburn M, Pei D, Xie T
    J Biol Chem, 2015;290(37):22782-94.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC Fluorescence
  8. Loss of tenomodulin results in reduced self-renewal and augmented senescence of tendon stem/progenitor cells.
    Authors: Alberton P, Dex S, Popov C, Shukunami C, Schieker M, Docheva D
    Stem Cells Dev, 2015;24(5):597-609.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IF
  9. Generation of transgene-free mouse induced pluripotent stem cells using an excisable lentiviral system.
    Authors: Varga E, Nemes C, Davis R, Ujhelly O, Klincumhom N, Polgar Z, Muenthaisong S, Pirity M, Dinnyes A
    Exp Cell Res, 2014;322(2):335-44.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Paraffin-embedded
  10. The histone H2A deubiquitinase Usp16 regulates embryonic stem cell gene expression and lineage commitment.
    Authors: Yang, Wei, Lee, Yun-Hwa, Jones, Amanda E, Woolnough, Jessica, Zhou, Dewang, Dai, Qian, Wu, Qiang, Giles, Keith E, Townes, Tim M, Wang, Hengbin
    Nat Commun, 2014;5(0):3818.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  11. STAT3 phosphorylation at tyrosine 705 and serine 727 differentially regulates mouse ESC fates.
    Authors: Huang G, Yan H, Ye S, Tong C, Ying Q
    Stem Cells, 2014;32(5):1149-60.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  12. Gbx2, a LIF/Stat3 target, promotes reprogramming to and retention of the pluripotent ground state.
    Authors: Tai, Chih-I, Ying, Qi-Long
    J Cell Sci, 2013;126(0):1093-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  13. Small RNA-mediated regulation of iPS cell generation.
    Authors: Li Z, Yang CS, Nakashima K, Rana TM
    EMBO J., 2011;30(5):823-34.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  14. LIF-independent JAK signalling to chromatin in embryonic stem cells uncovered from an adult stem cell disease.
    Authors: Griffiths DS, Li J, Dawson MA, Trotter MW, Cheng YH, Smith AM, Mansfield W, Liu P, Kouzarides T, Nichols J, Bannister AJ, Green AR, Gottgens B
    Nat. Cell Biol., 2011;13(1):13-21.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  15. Klf5 is involved in self-renewal of mouse embryonic stem cells.
    Authors: Parisi S, Passaro F, Aloia L, Manabe I, Nagai R, Pastore L, Russo T
    J. Cell. Sci., 2008;121(0):2629-34.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: ChIP
Expand to show all 15 Citations
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