Mouse NFIL3/E4BP4 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB8888
MAB8888-SP
Detection of NFIL3/E4BP4 in Mouse Splenocytes by Flow Cytometry.
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Mouse NFIL3/E4BP4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects NFIL3/E4BP4 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1218A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant mouse NFIL3/E4BP4
Lys140-Arg462
Accession # O08750
Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of NFIL3/E4BP4 in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of NFIL3/E4BP4 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with 100 ng/mL Recombinant Mouse IL‑2 (Catalog # 402-ML) overnight were stained with Rat Anti-Mouse NKp46/NCR1 APC‑conjugated Monoclonal Antibody (Catalog # FAB22252A) and either (A) Rabbit Anti-Mouse NFIL3/E4BP4 Monoclonal Antibody (Catalog # MAB8888) or
(B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: NFIL3/E4BP4

NFIL3 (Nuclear Factor, Interleukin 3 Regulated), also called E4BP4 (adenovirus E4 promoter binding protein 4), is an approximately 58 kDa transcription factor of the bZIP (basic leucine zipper) family. It is a transcriptional regulator expressed in T and pro-B lymphocytes and cardiomyocytes, binds IL-3 and promoters as a homodimer, and enhances cell survival. NFIL3 has also been shown to be involved in the development of Innate Lymphoid Cells (ILCs; 1,2). The 462 amino acid (aa) human NFIL3 contains a basic DNA binding domain (aa 73-95), a leucine zipper (aa 96-124), and a transcriptional repression domain (aa 299-363) that is potentially regulated by phosphorylation at Ser301 and Ser353. Within the region used as an immunogen, human NFIL3 shares 83% aa sequence identity with mouse and rat NFIL3.

Long Name
Nuclear Factor, Interleukin 3 Regulated
Entrez Gene IDs
4783 (Human); 18030 (Mouse); 114519 (Rat)
Alternate Names
E4BP4; E4BP4IL3BP1; IL3BP1; Interleukin-3 promoter transcriptional activator; Interleukin-3-binding protein 1; NFIL3; NF-IL3A; NFIL3AE4 promoter-binding protein 4; nuclear factor interleukin 3 regulated protein; nuclear factor interleukin-3-regulated protein; nuclear factor, interleukin 3 regulated; Transcriptional activator NF-IL3A

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