Mouse Pro‑HGF Activator Antibody

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HGF Activator in Mouse Intestine.
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Product Details
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Mouse Pro‑HGF Activator Antibody Summary

Species Reactivity
Detects mouse Pro-HGF Activator in direct ELISAs. In direct ELISAs, less than 4% cross-reactivity with the active form of recombinant mouse (rm) HGF Activator and no cross-reactivity with rmHGF propeptide and rmHGF is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse Pro-HGF Activator
Accession # Q9R098
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse Pro-HGF Activator
5-15 µg/mL
See below
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Pro-HGF Activator, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry HGF Activator antibody in Mouse Intestine by Immunohistochemistry (IHC-Fr). View Larger

HGF Activator in Mouse Intestine. HGF Activator was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse Pro-HGF Activator Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1715) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HGF Activator

Pro-HGF-A (Hepatocyte growth factor activator protein) is a 96 kDa member of the PA-FXII-HGFA family of enzymes. It is secreted by hepatocytes and is found in plasma at approximately 40 nM concentration. Other cells selectively reported to express HGF-A include astrocytes, endothelium, fibroblasts and macrophages. Upon activation, HGF-A is known to activate both proHGF and proMSP via proteolytic cleavage. The circulating 96 kDa form of mouse HGF-A represents an inactive 619 amino acid (aa) proform (aa 35-653). Within this proform exists one FN type II domain (aa 100-147), an EGF-like domain (aa 157-195), an FN type III domain (aa 197‑237), a second EGF-like domain (aa 238-276), a kringle domain (aa 283-364) and a functional peptidase S1 domain (aa 406-644). Activation of HGF-A typically occurs with a thrombin-mediated cleavage between Arg405-Ile406 and a KLK cleavage between Arg369-Val370. This creates a 34 kDa heterodimeric active product that contains a 2 kDa N-terminus (aa 370-405) disulfide linked to a 32 kDa C-terminus (aa 406-653). Once activated, the 34 kDa heterodimer binds to cell surface HAI‑1, rendering it unavailable to circulating HGF. When needed, this HGF-A:HAI-1 complex is released, HGF-A dissociates from HAI-1, and HGF-A is free to activate proHGF. Over aa 35-653, mouse HGF-A shares 92% and 82% aa identity with rat and human HGF-A, respectively.

Long Name
Hepatocyte Growth Factor Activator
Entrez Gene IDs
3083 (Human); 54426 (Mouse)
Alternate Names
HGF Activator; HGFA; Hgfac

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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