Mouse/Rat BCAM Antibody

Catalog # Availability Size / Price Qty
AF8299
AF8299-SP
Detection of Mouse and Rat BCAM by Western Blot.
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Product Details
Citations (4)
FAQs
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Mouse/Rat BCAM Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat BCAM in Western blots. In direct ELISAs, less than 50% cross-reactivity with recombinant human BCAM is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse BCAM
Met1-Ala541
Accession # Q9R069
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse and Rat BCAM antibody by Western Blot. View Larger

Detection of Mouse and Rat BCAM by Western Blot. Western blot shows lysates of mouse kidney tissue, mouse skeletal muscle tissue, rat heart tissue, and rat lung tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat BCAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8299) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for BCAM at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry BCAM antibody in Mouse Liver by Immunohistochemistry (IHC-Fr). View Larger

BCAM in Mouse Liver. BCAM was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse/Rat BCAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8299) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BCAM

Mouse BCAM (Basal cell adhesion molecule, B-CAM cell surface glycoprotein,Lutheran antigen, and CD239) is a 70-90 kDa membrane glycoprotein. BCAM has wide tissue distribution and is expressed on erythrocytes, the endothelium of blood vessels and on the basal layer of cells in the epithelia. The expression of BCAM in normal tissues is higher in fetal versus adult tissues. BCAM expression is also upregulated in activated keratinocytes and following malignant transformation in some cell types in vivo and in vitro. BCAM has been shown to be an adhesion molecule that binds laminin, a basement membrane protein involved in cell differentiation, adhesion, migration and proliferation. Over aa 1-541, mouse BCAM is 91% identical to rat BCAM.

Long Name
Basal Cell Adhesion Molecule
Entrez Gene IDs
4059 (Human); 57278 (Mouse)
Alternate Names
antigen identified by monoclonal F8; Auberger B antigen; basal cell adhesion molecule (Lu and Au blood groups); basal cell adhesion molecule (Lutheran blood group); basal cell adhesion molecule; B-CAM cell surface glycoprotein; BCAM; B-cell adhesion molecule; CD239 antigen; CD239; F8/G253 antigen; glycoprotein 95kDa; LUAU; Lutheran antigen; Lutheran blood group (Auberger b antigen included); Lutheran blood group glycoprotein; MSK19

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Citations for Mouse/Rat BCAM Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Apoptosis and tissue thinning contribute to symmetric cell division in the developing mouse epidermis in a nonautonomous way
    Authors: A Soffer, A Mahly, K Padmanabha, J Cohen, O Adir, E Loushi, Y Fuchs, SE Williams, C Luxenburg
    PloS Biology, 2022-08-15;20(8):e3001756.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IF, Western Blot
  2. Induction of osteogenesis by bone-targeted Notch activation
    Authors: C Xu, VV Dinh, K Kruse, HW Jeong, EC Watson, S Adams, F Berkenfeld, M Stehling, SJ Rasouli, R Fan, R Chen, I Bedzhov, Q Chen, K Kato, ME Pitulescu, RH Adams
    Elife, 2022-02-04;11(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Extensive remodeling of the extracellular matrix during aging contributes to age-dependent impairments of muscle stem cell functionality
    Authors: SC Schüler, JM Kirkpatric, M Schmidt, D Santinha, P Koch, S Di Sanzo, E Cirri, M Hemberg, A Ori, J von Maltza
    Cell Reports, 2021-06-08;35(10):109223.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Cell-matrix signals specify bone endothelial cells during developmental osteogenesis
    Authors: UH Langen, ME Pitulescu, JM Kim, R Enriquez-G, KK Sivaraj, AP Kusumbe, A Singh, J Di Russo, MG Bixel, B Zhou, L Sorokin, JM Vaquerizas, RH Adams
    Nat. Cell Biol, 2017-02-20;19(3):189-201.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody

WB,ELISA

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