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Mouse/Rat CD31/PECAM-1 Antibody

AF3628 61 Citations 7 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3628
AF3628-SP
Detection of Mouse CD31/PECAM‑1 by Western Blot.
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Product Details
Citations (61)
FAQs
Supplemental Products
Reviews (7)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat CD31/PECAM-1 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse CD31/PECAM-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human CD31 and recombinant porcine CD31 is observed. Detects mouse CD31 and rat CD31 in flow cytometry.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD31/PECAM-1
Glu18-Lys590
Accession # Q08481
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse CD31/PECAM‑1 by Western Blot. View Larger

Detection of Mouse CD31/PECAM‑1 by Western Blot. Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD31/PECAM‑1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of CD31/PECAM‑1 in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of CD31/PECAM‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Flow Cytometry Detection of CD31/PECAM‑1 in Rat Splenocytes by Flow Cytometry. View Larger

Detection of CD31/PECAM‑1 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Immunocytochemistry CD31/PECAM‑1 in bEnd.3 Mouse Cell Line. View Larger

CD31/PECAM‑1 in bEnd.3 Mouse Cell Line. CD31/PECAM‑1 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry CD31/PECAM‑1 in Mouse Embryo. View Larger

CD31/PECAM‑1 in Mouse Embryo. CD31/PECAM‑1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to developing endothelium. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunohistochemistry CD31/PECAM‑1 in Mouse Embryo. View Larger

CD31/PECAM‑1 in Mouse Embryo. CD31/PECAM‑1 was detected in immersion fixed frozen sections of mouse embryo (14 d.p.c.) using Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing guts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD31/PECAM-1

PECAM-1 (Platelet-Endothelial Cell Adhesion Molecule-1), also known as CD31, is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily (1, 2). Expression is restricted to cells involved in circulation, especially endothelial cells, platelets, monocytes, neutrophils and lymphocyte subsets. PECAM-1 is concentrated at cell-cell junctions and is required for Transendothelial Migration (TEM) (1-3). The Extracellular Domain (ECD) of PECAM-1 has ten potential N-linked glycosylation sites and six C2-type Ig-like domains, the first of which is critical for adhesion and extravasation (3, 4). The cytoplasmic domain contains Immunoregulatory Tyrosine-based Inhibitory and Switch Motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine-mediated engagement of SH2-containing signaling molecules (1, 5). Metalloproteinase-mediated ectodomain shedding occurs during apoptosis (6) but increased serum PECAM-1 ectodomain in HIV and active multiple sclerosis occurs independent of apoptosis (7, 8). In humans, expression of six isoforms with exon deletions in the cytoplasmic domain is tissue- and stage-specific, but full-length PECAM-1 is predominant. A form lacking the ITSM predominates in mouse (9). Mouse PECAM-1 ECD shows 77%, 63%, 63%, 63%, and 61% amino acid (aa) identity with rat, human, canine, porcine, and bovine PECAM-1, respectively. PECAM-1 participates with other adhesion molecules in some functions, but is the critical molecule for TEM. Homotypic PECAM-1 adhesion in trans, combined with cycling of PECAM-1 to and from surface-connected endothelial cell vesicles, leads leukocytes across endothelial tight junctions (3, 10). Homotypic adhesion and signaling functions also strongly suppress mitochondria-dependent apoptosis (11). In platelets, PECAM-1 is necessary for limiting thrombus formation (12) and promoting integrin-mediated clot retraction and platelet spreading (13), but mechanisms for these phenomena are unclear. PECAM-/- mice are deficient in chemokine-mediated chemotaxis (14).

References
  1. Ilan, N. and J.A. Madri (2003) Curr. Opin. Cell Biol. 15:515.
  2. Xie, Y. and W.A. Muller (1993) Proc. Natl. Acad. Sci. USA 90:5569.
  3. Liao, F. et al. (1997) J. Exp. Med. 185:1349.
  4. Nakada, M.T. et al. (2000) J. Immunol. 164:452.
  5. Chemnitz, J.M. et al. (2004) J. Immunol. 173:945.
  6. Ilan, N. et al. (2001) FASEB J. 15:362.
  7. Eugenin, E.A. et al. (2006) J. Leukoc. Biol. 79:444.
  8. Losy, J. et al. (1999) J. Neuroimmunol. 99:169.
  9. Wang, Y. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284:H1008.
  10. Mamdouh, Z. et al. (2003) Nature 421:748.
  11. Gao, C. et al. (2003) Blood 102:169.
  12. Falati, S. et al. (2006) Blood 107:535.
  13. Wee, J.L. and D.E. Jackson (2005) Blood 106:3816.
  14. Wu, Y. et al. (2005) J. Immunol. 175:3484.
Long Name
Platelet Endothelial Cell Adhesion Molecule 1
Entrez Gene IDs
5175 (Human); 18613 (Mouse); 29583 (Rat)
Alternate Names
adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; endoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule

Product Datasheets

Product Datasheet
COA
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Citations for Mouse/Rat CD31/PECAM-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

61 Citations: Showing 1 - 10
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  1. Wnt/?-catenin signaling regulates VE-cadherin-mediated anastomosis of brain capillaries by counteracting S1pr1 signaling
    Authors: K Hübner, P Cabochette, R Diéguez-Hu, C Wiesner, Y Wakayama, KS Grassme, M Hubert, S Guenther, HG Belting, M Affolter, RH Adams, B Vanhollebe, W Herzog
    Nat Commun, 2018;9(1):4860.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2018;15(1):39-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. SCL/TAL1 cooperates with Polycomb RYBP-PRC1 to suppress alternative lineages in blood-fated cells
    Authors: H Chagraoui, MS Kristianse, JP Ruiz, A Serra-Barr, J Richter, E Hall-Ponse, N Gray, D Waithe, K Clark, P Hublitz, E Repapi, G Otto, P Sopp, S Taylor, S Thongjuea, P Vyas, C Porcher
    Nat Commun, 2018;9(1):5375.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. Basal lamina remodeling at the skeletal muscle stem cell niche mediates stem cell self-renewal
    Authors: SS Rayagiri, D Ranaldi, A Raven, NIF Mohamad Az, O Lefebvre, PS Zammit, AG Borycki
    Nat Commun, 2018;9(1):1075.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. The tyrosine phosphatase SHP2 controls TGF?-induced STAT3 signaling to regulate fibroblast activation and fibrosis
    Authors: A Zehender, J Huang, AH Györfi, AE Matei, T Trinh-Minh, X Xu, YN Li, CW Chen, J Lin, C Dees, C Beyer, K Gelse, ZY Zhang, C Bergmann, A Ramming, W Birchmeier, O Distler, G Schett, JHW Distler
    Nat Commun, 2018;9(1):3259.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Prolonged systemic hyperglycemia does not cause pericyte loss and permeability at the mouse blood-brain barrier
    Authors: MA Mäe, T Li, G Bertuzzi, E Raschperge, M Vanlandewi, L He, K Nahar, A Dalheim, JJ Hofmann, B Laviña, A Keller, C Betsholtz, G Genové
    Sci Rep, 2018;8(1):17462.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Bones in human CYP26B1 deficiency and rats with hypervitaminosis A phenocopy Vegfa overexpression
    Authors: T Lind, R Lugano, AM Gustafson, M Norgård, A van Haerin, A Dimberg, H Melhus, SP Robertson, G Andersson
    Bone Rep, 2018;9(0):27-36.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  8. Syndecan-1 induction in lung microenvironment supports the establishment of breast tumor metastases
    Authors: C Chute, X Yang, K Meyer, N Yang, K O'Neil, I Kasza, K Eliceiri, C Alexander, A Friedl
    Breast Cancer Res., 2018;20(1):66.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Mesenchymal stem cells attenuate blood-brain barrier leakage after cerebral ischemia in mice
    Authors: Z Cheng, L Wang, M Qu, H Liang, W Li, Y Li, L Deng, Z Zhang, GY Yang
    J Neuroinflammation, 2018;15(1):135.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-F
  10. Local delivery of tetramethylpyrazine eliminates the senescent phenotype of bone marrow mesenchymal stromal cells and creates an anti-inflammatory and angiogenic environment in aging mice
    Authors: B Gao, X Lin, H Jing, J Fan, C Ji, Q Jie, C Zheng, D Wang, X Xu, Y Hu, W Lu, Z Luo, L Yang
    Aging Cell, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  11. Loss of pyruvate kinase M2 limits growth and triggers innate immune signaling in endothelial cells
    Authors: OA Stone, M El-Brolosy, K Wilhelm, X Liu, AM Romão, E Grillo, JKH Lai, S Günther, S Jeratsch, C Kuenne, IC Lee, T Braun, MM Santoro, JW Locasale, M Potente, DYR Stainier
    Nat Commun, 2018;9(1):4077.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Hindlimb Ischemia Impairs Endothelial Recovery and Increases Neointimal Proliferation in the Carotid Artery
    Authors: S Sorrentino, C Iaconetti, S De Rosa, A Polimeni, J Sabatino, C Gareri, F Passafaro, T Mancuso, L Tammè, C Mignogna, C Camastra, G Esposito, A Curcio, D Torella, C Indolfi
    Sci Rep, 2018;8(1):761.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. Angiopoietin/Tie2 axis regulates the age-at-injury cerebrovascular response to traumatic brain injury
    Authors: TR Brickler, A Hazy, F Guilhaume, R Dai, EJA Kowalski, R Dickerson, J Chen, X Wang, PD Morton, A Whittingto, A Ahmed, MH Theus
    J. Neurosci., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Endothelial repair is dependent on CD11c+ leukocytes to establish regrowing endothelial sheets with high cellular density
    Authors: U Yrlid, M Holm, M Levin, S Alsén, M Lindbom, L Glise, N Bergh, J Borén, P Fogelstran
    J. Leukoc. Biol., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. KSHV-induced ligand mediated activation of PDGF receptor-alpha drives Kaposi's sarcomagenesis
    Authors: LE Cavallin, Q Ma, J Naipauer, S Gupta, M Kurian, P Locatelli, P Romanelli, M Nadji, PJ Goldschmid, EA Mesri
    PLoS Pathog., 2018;14(7):e1007175.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Mesenchymal stem cells ameliorate hyperglycemia-induced endothelial injury through modulation of mitophagy
    Authors: W Zhu, Y Yuan, G Liao, L Li, J Liu, Y Chen, J Zhang, J Cheng, Y Lu
    Cell Death Dis, 2018;9(8):837.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow
  17. Tumour-vasculature development via endothelial-to-mesenchymal transition after radiotherapy controls CD44v6+ cancer cell and macrophage polarization
    Authors: SH Choi, AR Kim, JK Nam, JM Kim, JY Kim, HR Seo, HJ Lee, J Cho, YJ Lee
    Nat Commun, 2018;9(1):5108.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA
    Authors: MA Scavuzzo, J Chmielowie, D Yang, K Wamble, LS Chaboub, L Duraine, B Tepe, SM Glasgow, BR Arenkiel, C Brou, B Deneen, M Borowiak
    Cell Rep, 2018;25(13):3811-3827.e7.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IFS
  19. UHRF1 epigenetically orchestrates smooth muscle cell plasticity in arterial disease
    Authors: L Elia, P Kunderfran, P Carullo, M Vacchiano, FM Farina, IF Hall, S Mantero, C Panico, R Papait, G Condorelli, M Quintavall
    J. Clin. Invest., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Characterization of multi-cellular dynamics of angiogenesis and vascular remodelling by intravital imaging of the wounded mouse cornea
    Authors: Y Wang, Y Jin, B Laviña, L Jakobsson
    Sci Rep, 2018;8(1):10672.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. Attenuation of Retinal Vascular Development in Neonatal Mice Subjected to Hypoxic-Ischemic Encephalopathy
    Authors: IS Zaitoun, U Cikla, D Zafer, E Udho, R Almomani, A Suscha, P Cengiz, CM Sorenson, N Sheibani
    Sci Rep, 2018;0(1):9166.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. Genetic dissection of the miR-200-Zeb1 axis reveals its importance in tumor differentiation and invasion
    Authors: AC Title, SJ Hong, ND Pires, L Hasenöhrl, S Godbersen, N Stokar-Reg, DP Bartel, M Stoffel
    Nat Commun, 2018;9(1):4671.
    Species: Mouse
    Sample Types: Tissue Section
    Applications: IHC-P
  23. Peripheral myeloid cells contribute to brain injury in male neonatal mice
    Authors: PLP Smith, A Mottahedin, P Svedin, CJ Mohn, H Hagberg, J Ek, C Mallard
    J Neuroinflammation, 2018;15(1):301.
  24. cxcl12-engineered endothelial progenitor cells enhance neurogenesis and angiogenesis after ischemic brain injury in mice
    Authors: Y Li, S Chang, W Li, G Tang, Y Ma, Y Liu, F Yuan, Z Zhang, GY Yang, Y Wang
    Stem Cell Res Ther, 2018;9(1):139.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. Radiation-induced premature cellular senescence involved in glomerular diseases in rats
    Authors: S Aratani, M Tagawa, S Nagasaka, Y Sakai, A Shimizu, S Tsuruoka
    Sci Rep, 2018;8(1):16812.
    Species: Rat
    Sample Types: Tissue Section
    Applications: IHC-P
  26. Pentoxifylline decreases post-operative intra-abdominal adhesion formation in an animal model
    Authors: YL Yang, MG Lee, CC Lee, PI Su, CY Chi, CH Liu, MC Wu, ZS Yen, SC Chen
    PeerJ, 2018;6(0):e5434.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  27. Mesenchymal Precursor Cells in Adult Nerves Contribute to Mammalian Tissue Repair and Regeneration
    Authors: MJ Carr, JS Toma, APW Johnston, PE Steadman, SA Yuzwa, N Mahmud, PW Frankland, DR Kaplan, FD Miller
    Cell Stem Cell, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  28. Dosimetric evaluation of radionuclides for VCAM-1-targeted radionuclide therapy of early brain metastases
    Authors: N Falzone, NL Ackerman, LF Rosales, MA Bernal, X Liu, SG Peeters, MS Soto, A Corroyer-D, M Bernaudin, E Grimoin, O Touzani, NR Sibson, KA Vallis
    Theranostics, 2018;8(1):292-303.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  29. Activation of Hypoxia Signaling in Stromal Progenitors Impairs Kidney Development
    Authors: K Gerl, D Steppan, M Fuchs, C Wagner, C Willam, A Kurtz, B Kurt
    Am. J. Pathol., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  30. Natural compound bavachalcone promotes the differentiation of endothelial progenitor cells and neovascularization through the ROR?-erythropoietin-AMPK axis
    Authors: S Ling, RZ Ni, Y Yuan, YQ Dang, QM Zhou, S Liang, F Guo, W Feng, Y Chen, K Ikeda, Y Yamori, JW Xu
    Oncotarget, 2017;8(49):86188-86205.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  31. Silencing of galectin-1 inhibits retinal neovascularization and ameliorates retinal hypoxia in a murine model of oxygen-induced ischemic retinopathy
    Authors: N Yang, W Zhang, T He, Y Xing
    Exp. Eye Res, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  32. Gli1(+) Mesenchymal Stromal Cells Are a Key Driver of Bone Marrow Fibrosis and an Important Cellular Therapeutic Target
    Authors: RK Schneider, A Mullally, A Dugourd, F Peisker, R Hoogenboez, PMH Van Strien, EM Bindels, D Heckl, G Büsche, D Fleck, G Müller-New, J Wongboonsi, M Ventura Fe, VG Puelles, J Saez-Rodri, BL Ebert, BD Humphreys, R Kramann
    Cell Stem Cell, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC OCT-embedded
  33. Coronary angiogenic effect of long-term administration of Nigella sativa
    Authors: LI Al Asoom
    BMC Complement Altern Med, 2017;17(1):308.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  34. Pericyte-targeting prodrug overcomes tumor resistance to vascular disrupting agents
    Authors: M Chen, X Lei, C Shi, M Huang, X Li, B Wu, Z Li, W Han, B Du, J Hu, Q Nie, W Mai, N Ma, N Xu, X Zhang, C Fan, A Hong, M Xia, L Luo, A Ma, H Li, Q Yu, H Chen, D Zhang, W Ye
    J. Clin. Invest., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  35. Resident Arterial Cells and Circulating Bone Marrow-Derived Cells both Contribute to Intimal Hyperplasia in a Rat Allograft Carotid Transplantation Model
    Authors: Y He, X Xu, T Zhu, M Tang, J Mei, Y Si
    Cell. Physiol. Biochem., 2017;42(4):1303-1312.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  36. Endoglin prevents vascular malformation by regulating flow-induced cell migration and specification through VEGFR2 signalling
    Authors: Y Jin, L Muhl, M Burmakin, Y Wang, AC Duchez, C Betsholtz, HM Arthur, L Jakobsson
    Nat. Cell Biol., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  37. Brachyury drives formation of a distinct vascular branchpoint critical for fetal-placental arterial union in the mouse gastrula
    Authors: AM Rodriguez, DX Jin, AD Wolfe, MM Mikedis, L Wierenga, MP Hashmi, C Viebahn, KM Downs
    Dev. Biol., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  38. Immunofluorescence reveals unusual patterns of labelling for connexin43 localized to calbindin-D28K-positive interstitial cells in the pineal gland
    Authors: DD Tsao, SG Wang, BD Lynn, JI Nagy
    Eur. J. Neurosci., 2017;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-F
  39. Endoglin controls blood vessel diameter through endothelial cell shape changes in response to haemodynamic cues
    Authors: WW Sugden, R Meissner, T Aegerter-W, R Tsaryk, EV Leonard, J Bussmann, MJ Hamm, W Herzog, Y Jin, L Jakobsson, C Denz, AF Siekmann
    Nat. Cell Biol., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  40. Excessive dietary intake of vitamin A reduces skull bone thickness in mice
    Authors: T Lind, C Öhman, G Calounova, A Rasmusson, G Andersson, G Pejler, H Melhus
    PLoS ONE, 2017;12(4):e0176217.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  41. Clearance of Heparan Sulfate and Attenuation of CNS Pathology by Intracerebroventricular BMN 250 in Sanfilippo Type B Mice
    Authors: M Aoyagi-Sch, D Crippen-Ha, R Lawrence, J Vincelette, G Yogalingam, H Prill, BK Yip, B Baridon, C Vitelli, A Lee, O Gorostiza, EG Adintori, WC Minto, JL Van Vleet, B Yates, S Rigney, TM Christians, PMN Tiger, MJ Lo, J Holtzinger, PA Fitzpatric, JH LeBowitz, S Bullens, BE Crawford, S Bunting
    Mol Ther Methods Clin Dev, 2017;6(0):43-53.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  42. Gene therapy for a mouse model of glucose transporter-1 deficiency syndrome
    Authors: S Nakamura, H Osaka, SI Muramatsu, N Takino, M Ito, S Aoki, EF Jimbo, K Shimazaki, T Onaka, S Ohtsuki, T Terasaki, T Yamagata
    Mol Genet Metab Rep, 2017;10(0):67-74.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  43. Murine liver-resident group 1 innate lymphoid cells regulate optimal priming of anti-viral CD8+ T cells
    J Leukoc Biol, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC OCT-embedded
  44. Impacts of femoral artery and vein excision versus femoral artery excision on the hindlimb ischemic model in CD-1 mice
    Authors: Miao Chen
    Microvasc. Res, 2016;110(0):48-55.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  45. TLR2-mediated leukocyte trafficking to the developing brain
    J Leukoc Biol, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  46. The transcription factor Mesp1 interacts with cAMP-responsive element binding protein 1 (Creb1) and coactivates Ets variant 2 (Etv2) gene expression.
    Authors: Shi X, Zirbes K, Rasmussen T, Ferdous A, Garry M, Koyano-Nakagawa N, Garry D
    J Biol Chem, 2015;290(15):9614-25.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Not Specified
  47. Integrin beta1 controls VE-cadherin localization and blood vessel stability.
    Authors: Yamamoto H, Ehling M, Kato K, Kanai K, van Lessen M, Frye M, Zeuschner D, Nakayama M, Vestweber D, Adams R
    Nat Commun, 2015;6(0):6429.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  48. The formyl peptide receptor 1 exerts a tumor suppressor function in human gastric cancer by inhibiting angiogenesis.
    Authors: Prevete N, Liotti F, Visciano C, Marone G, Melillo R, de Paulis A
    Oncogene, 2015;34(29):3826-38.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Fresh
  49. Melanocyte-secreted fibromodulin promotes an angiogenic microenvironment.
    Authors: Adini, Irit, Ghosh, Kaustabh, Adini, Avner, Chi, Zai-Long, Yoshimura, Takeru, Benny, Ofra, Connor, Kip M, Rogers, Michael, Bazinet, Lauren, Birsner, Amy E, Bielenberg, Diane R, D'Amato, Robert J
    J Clin Invest, 2014;124(1):425-36.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  50. Formation of fenestrae in murine liver sinusoids depends on plasmalemma vesicle-associated protein and is required for lipoprotein passage.
    Authors: Herrnberger, Leonie, Hennig, Robert, Kremer, Werner, Hellerbrand, Claus, Goepferich, Achim, Kalbitzer, Hans Rob, Tamm, Ernst R
    PLoS ONE, 2014;9(12):e115005.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  51. ITE and TCDD differentially regulate the vascular remodeling of rat placenta via the activation of AhR.
    Authors: Wu Y, Chen X, Zhou Q, He Q, Kang J, Zheng J, Wang K, Duan T
    PLoS ONE, 2014;9(1):e86549.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  52. Characterization of the adverse effects of nicotine on placental development: in vivo and in vitro studies.
    Authors: Holloway, A C, Salomon, A, Soares, M J, Garnier, V, Raha, S, Sergent, F, Nicholson, C J, Feige, J J, Benharouga, M, Alfaidy, N
    Am J Physiol Endocrinol Metab, 2014;306(4):E443-56.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: WB
  53. Periadventitial application of rapamycin-loaded nanoparticles produces sustained inhibition of vascular restenosis.
    Authors: Shi, Xudong, Chen, Guojun, Guo, Lian-Wan, Si, Yi, Zhu, Men, Pilla, Srikanth, Liu, Bo, Gong, Shaoqin, Kent, K Craig
    PLoS ONE, 2014;9(2):e89227.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  54. Anticancer effects of gemcitabine are enhanced by co-administered iRGD peptide in murine pancreatic cancer models that overexpressed neuropilin-1.
    Authors: Akashi Y, Oda T, Ohara Y, Miyamoto R, Kurokawa T, Hashimoto S, Enomoto T, Yamada K, Satake M, Ohkohchi N
    Br J Cancer, 2014;110(6):1481-7.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC OCT-embedded
  55. MicroRNA-210 overexpression induces angiogenesis and neurogenesis in the normal adult mouse brain.
    Authors: Zeng, L, He, X, Wang, Y, Tang, Y, Zheng, C, Cai, H, Liu, J, Wang, Y, Fu, Y, Yang, G-Y
    Gene Ther, 2014;21(1):37-43.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-OCT embedded
  56. Peroxisome proliferator-activated receptor alpha protects capillary pericytes in the retina.
    Authors: Ding L, Cheng R, Hu Y, Takahashi Y, Jenkins A, Keech A, Humphries K, Gu X, Elliott M, Xia X, Ma J
    Am J Pathol, 2014;184(10):2709-20.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IF
  57. Endoglin regulates the activation and quiescence of endothelium by participating in canonical and non-canonical TGF-beta signaling pathways.
    Authors: Park S, Dimaio T, Liu W, Wang S, Sorenson C, Sheibani N
    J Cell Sci, 2013;126(0):1392-405.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  58. TGF-beta mediates homing of bone marrow-derived human mesenchymal stem cells to glioma stem cells.
    Authors: Shinojima N, Hossain A, Takezaki T, Fueyo J, Gumin J, Gao F, Nwajei F, Marini F, Andreeff M, Kuratsu J, Lang F
    Cancer Res, 2013;73(7):2333-44.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  59. The critical role of agrin in the hematopoietic stem cell niche.
    Authors: Mazzon C, Anselmo A, Cibella J, Soldani C, Destro A, Kim N, Roncalli M, Burden SJ, Dustin ML, Sarukhan A, Viola A
    Blood, 2011;118(10):2733-42.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  60. Dysfunctional microvasculature as a consequence of shb gene inactivation causes impaired tumor growth.
    Authors: Funa NS, Kriz V, Zang G, Calounova G, Akerblom B, Mares J, Larsson E, Sun Y, Betsholtz C, Welsh M
    Cancer Res., 2009;69(5):2141-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  61. Crosstalk between keratinocytes and adaptive immune cells in an IkappaBalpha protein-mediated inflammatory disease of the skin.
    Authors: Rebholz B, Haase I, Eckelt B, Paxian S, Flaig MJ, Ghoreschi K, Nedospasov SA, Mailhammer R, Debey-Pascher S, Schultze JL, Weindl G, Forster I, Huss R, Stratis A, Ruzicka T, Rocken M, Pfeffer K, Schmid RM, Rupec RA
    Immunity, 2007;27(2):296-307.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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Reviews for Mouse/Rat CD31/PECAM-1 Antibody

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Mouse/Rat CD31/PECAM-1 Antibody
By Anonymous on 08/20/2018
Application: IHC Sample Tested: Liver tissue Species: Rat

FFPE rat liver stained using AF3628 at 15 µg/mL overnight at 4C. Epitope retrieval was performed at 95 C for 15 minutes in citrate buffer pH 6.0. Secondary was donkey anti-goat Alexa Fluor 555 (psuedocolored red).

Immunohistochemistry Mouse/Rat CD31/PECAM-1 Antibody AF3628
Mouse/Rat CD31/PECAM-1 Antibody
By Anonymous on 06/02/2018
Application: CLARITY deep tissue imaging Sample Tested: Adult kidney Species: Mouse

Optically cleared 1 mm mouse kidney section
Imaged by diSPIM in 60% TDE with 17X lens

Secondary antibody is donkey anti goat alexa 488

Red is Tubulin Beta 3 stained by alexa 568

CLARITY deep tissue imaging Mouse/Rat CD31/PECAM-1 Antibody AF3628
Mouse/Rat CD31/PECAM-1 Antibody
By Anonymous on 04/05/2018
Application: ICC/IF Sample Tested: Adult brain Species: Mouse

Excellent!

Immunocytochemistry/Immunofluorescence Mouse/Rat CD31/PECAM-1 Antibody AF3628
Mouse/Rat CD31/PECAM-1 Antibody
By Anonymous on 03/26/2018
Application: IHC Sample Tested: Liver tissue Species: Mouse

FFPE mouse liver stained O/N at 4C with AF3826 at 10 ug/mL in 0.3% PBS-Tx. Secondary Donkey anti-goat Alexa Fluor 488 was applied for 1 hr RT at 2 ug/mL in PBS-Tx. Citrate buffer pH 6.0 used for epitope retrieval.

Immunohistochemistry Mouse/Rat CD31/PECAM-1 Antibody AF3628
Mouse/Rat CD31/PECAM-1 Antibody
By Anonymous on 09/12/2017
Application: IHC Sample Tested: Embryonic kidney Species: Mouse
Immunohistochemistry Mouse/Rat CD31/PECAM-1 Antibody AF3628
Mouse/Rat CD31/PECAM-1 Antibody
By Anonymous on 06/29/2017
Application: IHC-P Sample Tested: Formalin Fixed Paraffin Embedded Mouse Liver Species: Mouse

WD [1:500], HIER with DIVA; secondary ab RTU Polymer-HRP

Immunohistochemistry-Paraffin Mouse/Rat CD31/PECAM-1 Antibody AF3628
Mouse/Rat CD31/PECAM-1 Antibody
By Anonymous on 08/08/2016
Application: IHC Sample Tested: Embryo tissue Species: Mouse

E9.5 mouse embryonic tissue, whole mount staining with 1:100 dilution, Alex Fluor 594 donkey anti-goat with 1:400 dilution.

Immunohistochemistry Mouse/Rat CD31/PECAM-1 Antibody AF3628