Mouse/Rat CD31/PECAM-1 Antibody Summary
Glu18-Lys590
Accession # Q08481
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Mouse CD31/PECAM‑1 by Western Blot. Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD31/PECAM-1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of CD31/PECAM‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Detection of CD31/PECAM‑1 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

CD31/PECAM‑1 in bEnd.3 Mouse Cell Line. CD31/PECAM-1 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

CD31/PECAM‑1 in Mouse Embryo. CD31/PECAM-1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Goat Anti-Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to developing endothelium. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

CD31/PECAM‑1 in Mouse Embryo. CD31/PECAM-1 was detected in immersion fixed frozen sections of mouse embryo (14 d.p.c.) using Goat Anti-Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing guts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD31/PECAM-1
PECAM-1 (Platelet-Endothelial Cell Adhesion Molecule-1), also known as CD31, is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily (1, 2). Expression is restricted to cells involved in circulation, especially endothelial cells, platelets, monocytes, neutrophils and lymphocyte subsets. PECAM-1 is concentrated at cell-cell junctions and is required for Transendothelial Migration (TEM) (1-3). The Extracellular Domain (ECD) of PECAM-1 has ten potential N-linked glycosylation sites and six C2-type Ig-like domains, the first of which is critical for adhesion and extravasation (3, 4). The cytoplasmic domain contains Immunoregulatory Tyrosine-based Inhibitory and Switch Motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine-mediated engagement of SH2-containing signaling molecules (1, 5). Metalloproteinase-mediated ectodomain shedding occurs during apoptosis (6) but increased serum PECAM-1 ectodomain in HIV and active multiple sclerosis occurs independent of apoptosis (7, 8). In humans, expression of six isoforms with exon deletions in the cytoplasmic domain is tissue- and stage-specific, but full-length PECAM-1 is predominant. A form lacking the ITSM predominates in mouse (9). Mouse PECAM-1 ECD shows 77%, 63%, 63%, 63%, and 61% amino acid (aa) identity with rat, human, canine, porcine, and bovine PECAM-1, respectively. PECAM-1 participates with other adhesion molecules in some functions, but is the critical molecule for TEM. Homotypic PECAM-1 adhesion in trans, combined with cycling of PECAM-1 to and from surface-connected endothelial cell vesicles, leads leukocytes across endothelial tight junctions (3, 10). Homotypic adhesion and signaling functions also strongly suppress mitochondria-dependent apoptosis (11). In platelets, PECAM-1 is necessary for limiting thrombus formation (12) and promoting integrin-mediated clot retraction and platelet spreading (13), but mechanisms for these phenomena are unclear. PECAM-/- mice are deficient in chemokine-mediated chemotaxis (14).
- Ilan, N. and J.A. Madri (2003) Curr. Opin. Cell Biol. 15:515.
- Xie, Y. and W.A. Muller (1993) Proc. Natl. Acad. Sci. USA 90:5569.
- Liao, F. et al. (1997) J. Exp. Med. 185:1349.
- Nakada, M.T. et al. (2000) J. Immunol. 164:452.
- Chemnitz, J.M. et al. (2004) J. Immunol. 173:945.
- Ilan, N. et al. (2001) FASEB J. 15:362.
- Eugenin, E.A. et al. (2006) J. Leukoc. Biol. 79:444.
- Losy, J. et al. (1999) J. Neuroimmunol. 99:169.
- Wang, Y. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284:H1008.
- Mamdouh, Z. et al. (2003) Nature 421:748.
- Gao, C. et al. (2003) Blood 102:169.
- Falati, S. et al. (2006) Blood 107:535.
- Wee, J.L. and D.E. Jackson (2005) Blood 106:3816.
- Wu, Y. et al. (2005) J. Immunol. 175:3484.
Product Datasheets
Citations for Mouse/Rat CD31/PECAM-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
100
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Eradication of tumor growth by delivering novel photothermal selenium-coated tellurium nanoheterojunctions
Authors: S Chen, C Xing, D Huang, C Zhou, B Ding, Z Guo, Z Peng, D Wang, X Zhu, S Liu, Z Cai, J Wu, J Zhao, Z Wu, Y Zhang, C Wei, Q Yan, H Wang, D Fan, L Liu, H Zhang, Y Cao
Sci Adv, 2020;6(15):eaay6825.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Brain Endothelial Cells Are Exquisite Sensors of Age-Related Circulatory Cues
Authors: MB Chen, AC Yang, H Yousef, D Lee, W Chen, N Schaum, B Lehallier, SR Quake, T Wyss-Coray
Cell Rep, 2020;30(13):4418-4432.e4.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Identification of a novel tumor angiogenesis inhibitor targeting Shh/Gli1 signaling pathway in Non-small cell lung cancer
Authors: X Lei, Y Zhong, L Huang, S Li, J Fu, L Zhang, Y Zhang, Q Deng, X Yu
Cell Death Dis, 2020;11(4):232.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Weekends-Off Lenvatinib for Unresectable Hepatocellular Carcinoma Improves Therapeutic Response and Tolerability toward Adverse Events
Authors: H Iwamoto, H Suzuki, S Shimose, T Niizeki, M Nakano, T Shirono, S Okamura, Y Noda, N Kamachi, T Nakamura, A Masuda, T Sakaue, T Tanaka, D Nakano, M Sakai, T Yamaguchi, R Kuromatsu, H Koga, T Torimura
Cancers (Basel), 2020;12(4):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Imatinib Sets Pericyte Mosaic in the Retina
Authors: T Kovács-Öll, E Ivanova, G Szarka, ÁJ Tengölics, B Völgyi, BT Sagdullaev
Int J Mol Sci, 2020;21(7):.
Species: Mouse
Sample Types: Tissue
Applications: IHC-P -
Macrophage M2 polarization induced by exosomes from adipose-derived stem cells contributes to the exosomal proangiogenic effect on mouse ischemic hindlimb
Authors: D Zhu, TK Johnson, Y Wang, M Thomas, K Huynh, Q Yang, VC Bond, YE Chen, D Liu
Stem Cell Res Ther, 2020;11(1):162.
Species: Mouse
Sample Types: Tissue
Applications: IHC-Fr -
Blocking endothelial apoptosis revascularises the retina in a model of ischemic retinopathy
Authors: ZL Grant, L Whitehead, VHY Wong, Z He, RY Yan, AR Miles, AV Benest, DO Bates, C Prahst, K Bentley, BV Bui, RC Symons, L Coultas
J. Clin. Invest., 2020;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IIHC -
Neutrophil extracellular traps released by neutrophils impair revascularization and vascular remodeling after stroke
Authors: L Kang, H Yu, X Yang, Y Zhu, X Bai, R Wang, Y Cao, H Xu, H Luo, L Lu, MJ Shi, Y Tian, W Fan, BQ Zhao
Nat Commun, 2020;11(1):2488.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Farnesoid X receptor knockout protects brain against ischemic injury through reducing neuronal apoptosis in mice
Authors: HM Shan, M Zang, Q Zhang, RB Shi, XJ Shi, M Mamtilahun, C Liu, LL Luo, X Tian, Z Zhang, GY Yang, Y Tang, J Pu, Y Wang
J Neuroinflammation, 2020;17(1):164.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
The Paracrine Role of Endothelial Cells in Bone Formation via CXCR4/SDF-1 Pathway
Authors: T Tamari, R Kawar-Jara, O Doppelt, B Giladi, N Sabbah, H Zigdon-Gil
Cells, 2020;9(6):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Mutations that prevent caspase cleavage of RIPK1 cause autoinflammatory disease
Authors: N Lalaoui, SE Boyden, H Oda, GM Wood, DL Stone, D Chau, L Liu, M Stoffels, T Kratina, KE Lawlor, KJM Zaal, PM Hoffmann, N Etemadi, K Shield-Art, C Biben, WL Tsai, MD Blake, HS Kuehn, D Yang, H Anderton, N Silke, L Wachsmuth, L Zheng, NS Moura, DB Beck, G Gutierrez-, AK Ombrello, GP Pinto-Pata, AJ Kueh, MJ Herold, C Hall, H Wang, JJ Chae, NI Dmitrieva, M McKenzie, A Light, BK Barham, A Jones, TM Romeo, Q Zhou, I Aksentijev, JC Mullikin, AJ Gross, AK Shum, ED Hawkins, SL Masters, MJ Lenardo, M Boehm, SD Rosenzweig, M Pasparakis, AK Voss, M Gadina, DL Kastner, J Silke
Nature, 2020;577(7788):103-108.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Functional and Evolutionary Diversification of Otx2 and Crx in Vertebrate Retinal Photoreceptor and Bipolar Cell Development
Authors: H Yamamoto, T Kon, Y Omori, T Furukawa
Cell Rep, 2020;30(3):658-671.e5.
Species: Mouse
Sample Types: Whole Tissue
Applications: IF -
Transfusion of Resting Platelets Reduces Brain Hemorrhage After Intracerebral Hemorrhage and tPA-Induced Hemorrhage After Cerebral Ischemia
Authors: H Luo, L Wei, L Lu, L Kang, Y Cao, X Yang, X Bai, W Fan, BQ Zhao
Front Neurosci, 2019;13(0):338.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes
Authors: R Diéguez-Hu, K Kato, BD Giaimo, M Nieminen-K, H Arf, F Ferrante, M Bartkuhn, T Zimmermann, MG Bixel, HM Eilken, S Adams, T Borggrefe, P Vajkoczy, RH Adams
Nat Commun, 2019;10(1):2817.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Administration of Tramadol or Buprenorphine via the drinking water for post-operative analgesia in a mouse-osteotomy model
Authors: P Jirkof, M Durst, R Klopfleisc, R Palme, C Thöne-Rein, F Buttgereit, K Schmidt-Bl, A Lang
Sci Rep, 2019;9(1):10749.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Endothelial proteolytic activity and interaction with non-resorbing osteoclasts mediate bone elongation
Authors: SG Romeo, KM Alawi, J Rodrigues, A Singh, AP Kusumbe, SK Ramasamy
Nat. Cell Biol., 2019;21(4):430-441.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Semaphorin-3A protects against neointimal hyperplasia after vascular injury
Authors: JH Wu, YF Zhou, CD Hong, AQ Chen, Y Luo, L Mao, YP Xia, QW He, HJ Jin, M Huang, YN Li, B Hu
EBioMedicine, 2019;39(0):95-108.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Tumour pH and protein concentration contribute to the signal of amide proton transfer magnetic resonance imaging
Authors: KJ Ray, MA Simard, JR Larkin, J Coates, P Kinchesh, SC Smart, GS Higgins, M Chappell, N Sibson
Cancer Res., 2019;0(0):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
PU.1 controls fibroblast polarization and tissue fibrosis
Authors: T Wohlfahrt, S Rauber, S Uebe, M Luber, A Soare, A Ekici, S Weber, AE Matei, CW Chen, C Maier, E Karouzakis, HP Kiener, E Pachera, C Dees, C Beyer, C Daniel, K Gelse, AE Kremer, E Naschberge, M Stürzl, F Butter, M Sticherlin, S Finotto, A Kreuter, MH Kaplan, A Jüngel, S Gay, SL Nutt, DW Boykin, GMK Poon, O Distler, G Schett, JHW Distler, A Ramming
Nature, 2019;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Endothelial cells are a source of Nestin expression in Pulmonary Arterial Hypertension
Authors: AR Bhagwani, S Hultman, D Farkas, R Moncayo, K Dandamudi, AK Zadu, CD Cool, L Farkas
PLoS ONE, 2019;14(3):e0213890.
Species: Rat
Sample Types: Whole Cells
Applications: Cell Selection -
Long-term mucosal injury and repair in a murine model of pelvic radiotherapy
Authors: DK Malipatlol, P Patel, F Sjöberg, S Devarakond, M Kalm, E Angenete, EB Lindskog, R Grandér, L Persson, A Stringer, U Wilderäng, J Swanpalmer, HG Kuhn, G Steineck, C Bull
Sci Rep, 2019;9(1):13803.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
UTX/KDM6A Deletion Promotes Recovery of Spinal Cord Injury by Epigenetically Regulating Vascular Regeneration
Authors: S Ni, Z Luo, L Jiang, Z Guo, P Li, X Xu, Y Cao, C Duan, T Wu, C Li, H Lu, J Hu
Mol. Ther., 2019;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Cellular Mechanisms of Angiogenesis in Neonatal Rat Models of Retinal Neurodegeneration
Authors: D Asano, M Hokazono, S Hirano, A Morita, T Nakahara
Int J Mol Sci, 2019;20(19):.
Species: Rat
Sample Types: Whole Cells
Applications: Cell Culture -
TAFI deficiency causes maladaptive vascular remodeling after hemophilic joint bleeding
Authors: T Wyseure, T Yang, JY Zhou, EJ Cooke, B Wanko, M Olmer, R Agashe, Y Morodomi, N Behrendt, M Lotz, J Morser, A von Drygal, LO Mosnier
JCI Insight, 2019;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Mitochondrial calcium exchange links metabolism with the epigenome to control cellular differentiation
Authors: AA Lombardi, AA Gibb, E Arif, DW Kolmetzky, D Tomar, TS Luongo, P Jadiya, EK Murray, PK Lorkiewicz, G Hajnóczky, E Murphy, ZP Arany, DP Kelly, KB Margulies, BG Hill, JW Elrod
Nat Commun, 2019;10(1):4509.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Secreted protein acidic and rich in cysteine mediates active targeting of human serum albumin in U87MG xenograft mouse models
Authors: CR Park, JH Jo, MG Song, JY Park, YH Kim, H Youn, SH Paek, JK Chung, JM Jeong, YS Lee, KW Kang
Theranostics, 2019;9(24):7447-7457.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Predicting Angiogenesis by Endothelial Progenitor Cells Relying on In-Vitro Function Assays and VEGFR-2 Expression Levels
Authors: N Sabbah, T Tamari, R Elimelech, O Doppelt, U Rudich, H Zigdon-Gil
Biomolecules, 2019;9(11):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Graft immaturity and safety concerns in transplanted human kidney organoids
Authors: SA Nam, E Seo, JW Kim, HW Kim, HL Kim, K Kim, TM Kim, JH Ju, IG Gomez, K Uchimura, BD Humphreys, CW Yang, JY Lee, J Kim, DW Cho, BS Freedman, YK Kim
Exp. Mol. Med., 2019;51(11):145.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Cleaved CD31 as a target for in vivo molecular imaging of inflammation
Authors: J Vigne, S Bay, R Aid-Launai, G Pariscoat, G Rucher, J Sénémaud, A Truffier, N Anizan, G Even, C Ganneau, F Andreata, M Le Borgne, A Nicoletti, D Le Guludec, G Caligiuri, F Rouzet
Sci Rep, 2019;9(1):19560.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-P -
Endothelial EphB4 maintains vascular integrity and transport function in adult heart
Authors: G Luxán, J Stewen, N Díaz, K Kato, SK Maney, A Aravamudha, F Berkenfeld, N Nagelmann, HC Drexler, D Zeuschner, C Faber, H Schillers, S Hermann, J Wiseman, JM Vaquerizas, ME Pitulescu, RH Adams
Elife, 2019;8(0):.
Species: Mouse
Sample Types: Tissue Lysates
Applications: IHC -
Cardiomyocyte d-dopachrome tautomerase protects against heart failure
Authors: Y Ma, KN Su, D Pfau, VS Rao, X Wu, X Hu, L Leng, X Du, M Piecychna, K Bedi, SG Campbell, A Eichmann, JM Testani, KB Margulies, R Bucala, LH Young
JCI Insight, 2019;4(17):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Engineering transferrable microvascular meshes for subcutaneous islet transplantation
Authors: W Song, A Chiu, LH Wang, RE Schwartz, B Li, N Bouklas, DT Bowers, D An, SH Cheong, JA Flanders, Y Pardo, Q Liu, X Wang, VK Lee, G Dai, M Ma
Nat Commun, 2019;10(1):4602.
Species: Mouse
Sample Types: Microvascular Mesh
Applications: IHC -
Single-cell analysis reveals T cell infiltration in old neurogenic niches
Authors: BW Dulken, MT Buckley, P Navarro Ne, N Saligrama, R Cayrol, DS Leeman, BM George, SC Boutet, K Hebestreit, JV Pluvinage, T Wyss-Coray, IL Weissman, H Vogel, MM Davis, A Brunet
Nature, 2019;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Genetic dissection of the miR-200-Zeb1 axis reveals its importance in tumor differentiation and invasion
Authors: AC Title, SJ Hong, ND Pires, L Hasenöhrl, S Godbersen, N Stokar-Reg, DP Bartel, M Stoffel
Nat Commun, 2018;9(1):4671.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA
Authors: MA Scavuzzo, J Chmielowie, D Yang, K Wamble, LS Chaboub, L Duraine, B Tepe, SM Glasgow, BR Arenkiel, C Brou, B Deneen, M Borowiak
Cell Rep, 2018;25(13):3811-3827.e7.
Species: Mouse
Sample Types: Whole Tissue
Applications: Ifs -
SCL/TAL1 cooperates with Polycomb RYBP-PRC1 to suppress alternative lineages in blood-fated cells
Authors: H Chagraoui, MS Kristianse, JP Ruiz, A Serra-Barr, J Richter, E Hall-Ponse, N Gray, D Waithe, K Clark, P Hublitz, E Repapi, G Otto, P Sopp, S Taylor, S Thongjuea, P Vyas, C Porcher
Nat Commun, 2018;9(1):5375.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Tumour-vasculature development via endothelial-to-mesenchymal transition after radiotherapy controls CD44v6+ cancer cell and macrophage polarization
Authors: SH Choi, AR Kim, JK Nam, JM Kim, JY Kim, HR Seo, HJ Lee, J Cho, YJ Lee
Nat Commun, 2018;9(1):5108.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Mesenchymal Precursor Cells in Adult Nerves Contribute to Mammalian Tissue Repair and Regeneration
Authors: MJ Carr, JS Toma, APW Johnston, PE Steadman, SA Yuzwa, N Mahmud, PW Frankland, DR Kaplan, FD Miller
Cell Stem Cell, 2018;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Radiation-induced premature cellular senescence involved in glomerular diseases in rats
Authors: S Aratani, M Tagawa, S Nagasaka, Y Sakai, A Shimizu, S Tsuruoka
Sci Rep, 2018;8(1):16812.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-P -
Peripheral myeloid cells contribute to brain injury in male neonatal mice
Authors: PLP Smith, A Mottahedin, P Svedin, CJ Mohn, H Hagberg, J Ek, C Mallard
J Neuroinflammation, 2018;15(1):301.
-
A Bayesian Framework for Generalized Linear Mixed Modeling Identifies New Candidate Loci for Late-Onset Alzheimer's Disease
Authors: X Wang, VM Philip, G Ananda, CC White, A Malhotra, PJ Michalski, KRM Karuturi, SR Chintalapu, C Acklin, M Sasner, DA Bennett, PL De Jager, GR Howell, GW Carter
Genetics, 2018;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Wnt/?-catenin signaling regulates VE-cadherin-mediated anastomosis of brain capillaries by counteracting S1pr1 signaling
Authors: K Hübner, P Cabochette, R Diéguez-Hu, C Wiesner, Y Wakayama, KS Grassme, M Hubert, S Guenther, HG Belting, M Affolter, RH Adams, B Vanhollebe, W Herzog
Nat Commun, 2018;9(1):4860.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Positive-feedback regulation of subchondral H-type vessel formation by chondrocyte promotes osteoarthritis development in mice
Authors: J Lu, H Zhang, D Cai, C Zeng, P Lai, Y Shao, H Fang, D Li, J Ouyang, C Zhao, D Xie, B Huang, J Yang, Y Jiang, X Bai
J. Bone Miner. Res., 2018;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Loss of pyruvate kinase M2 limits growth and triggers innate immune signaling in endothelial cells
Authors: OA Stone, M El-Brolosy, K Wilhelm, X Liu, AM Romão, E Grillo, JKH Lai, S Günther, S Jeratsch, C Kuenne, IC Lee, T Braun, MM Santoro, JW Locasale, M Potente, DYR Stainier
Nat Commun, 2018;9(1):4077.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Type I interferons instigate fetal demise after Zika virus infection
Authors: LJ Yockey, KA Jurado, N Arora, A Millet, T Rakib, KM Milano, AK Hastings, E Fikrig, Y Kong, TL Horvath, S Weatherbee, HJ Kliman, CB Coyne, A Iwasaki
Sci Immunol, 2018;3(19):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Gellan Gum-based luminal fillers for peripheral nerve regeneration: an in vivo study in the rat sciatic nerve repair model
Authors: CR Carvalho, S Wrobel, C Meyer, C Brandenber, IF Cengiz, R López-Cebr, J Silva-Corr, G Ronchi, RL Reis, C Grothe, JM Oliveira, K Haastert-T
Biomater Sci, 2018;0(0):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-P -
Differential role of Interleukin-1 and Interleukin-6 in K-Ras-driven pancreatic carcinoma undergoing mesenchymal transition
Authors: I Siddiqui, M Erreni, MA Kamal, C Porta, F Marchesi, S Pesce, F Pasqualini, S Schiarea, C Chiabrando, A Mantovani, P Allavena
Oncoimmunology, 2018;7(2):e1388485.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Pr -
Intratumoral administration of IL2- and TNF-based fusion proteins cures cancer without establishing protective immunity
Authors: B Ziffels, F Pretto, D Neri
Immunotherapy, 2018;10(3):177-188.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Prolonged systemic hyperglycemia does not cause pericyte loss and permeability at the mouse blood-brain barrier
Authors: MA Mäe, T Li, G Bertuzzi, E Raschperge, M Vanlandewi, L He, K Nahar, A Dalheim, JJ Hofmann, B Laviña, A Keller, C Betsholtz, G Genové
Sci Rep, 2018;8(1):17462.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Angiopoietin/Tie2 axis regulates the age-at-injury cerebrovascular response to traumatic brain injury
Authors: TR Brickler, A Hazy, F Guilhaume, R Dai, EJA Kowalski, R Dickerson, J Chen, X Wang, PD Morton, A Whittingto, A Ahmed, MH Theus
J. Neurosci., 2018;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Dosimetric evaluation of radionuclides for VCAM-1-targeted radionuclide therapy of early brain metastases
Authors: N Falzone, NL Ackerman, LF Rosales, MA Bernal, X Liu, SG Peeters, MS Soto, A Corroyer-D, M Bernaudin, E Grimoin, O Touzani, NR Sibson, KA Vallis
Theranostics, 2018;8(1):292-303.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Hindlimb Ischemia Impairs Endothelial Recovery and Increases Neointimal Proliferation in the Carotid Artery
Authors: S Sorrentino, C Iaconetti, S De Rosa, A Polimeni, J Sabatino, C Gareri, F Passafaro, T Mancuso, L Tammè, C Mignogna, C Camastra, G Esposito, A Curcio, D Torella, C Indolfi
Sci Rep, 2018;8(1):761.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-P -
Characterization of multi-cellular dynamics of angiogenesis and vascular remodelling by intravital imaging of the wounded mouse cornea
Authors: Y Wang, Y Jin, B Laviña, L Jakobsson
Sci Rep, 2018;8(1):10672.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
cxcl12-engineered endothelial progenitor cells enhance neurogenesis and angiogenesis after ischemic brain injury in mice
Authors: Y Li, S Chang, W Li, G Tang, Y Ma, Y Liu, F Yuan, Z Zhang, GY Yang, Y Wang
Stem Cell Res Ther, 2018;9(1):139.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Basal lamina remodeling at the skeletal muscle stem cell niche mediates stem cell self-renewal
Authors: SS Rayagiri, D Ranaldi, A Raven, NIF Mohamad Az, O Lefebvre, PS Zammit, AG Borycki
Nat Commun, 2018;9(1):1075.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Endothelial repair is dependent on CD11c+ leukocytes to establish regrowing endothelial sheets with high cellular density
Authors: U Yrlid, M Holm, M Levin, S Alsén, M Lindbom, L Glise, N Bergh, J Borén, P Fogelstran
J. Leukoc. Biol., 2018;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Local delivery of tetramethylpyrazine eliminates the senescent phenotype of bone marrow mesenchymal stromal cells and creates an anti-inflammatory and angiogenic environment in aging mice
Authors: B Gao, X Lin, H Jing, J Fan, C Ji, Q Jie, C Zheng, D Wang, X Xu, Y Hu, W Lu, Z Luo, L Yang
Aging Cell, 2018;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Bones in human CYP26B1 deficiency and rats with hypervitaminosis A phenocopy Vegfa overexpression
Authors: T Lind, R Lugano, AM Gustafson, M Norgård, A van Haerin, A Dimberg, H Melhus, SP Robertson, G Andersson
Bone Rep, 2018;9(0):27-36.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
UHRF1 epigenetically orchestrates smooth muscle cell plasticity in arterial disease
Authors: L Elia, P Kunderfran, P Carullo, M Vacchiano, FM Farina, IF Hall, S Mantero, C Panico, R Papait, G Condorelli, M Quintavall
J. Clin. Invest., 2018;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Syndecan-1 induction in lung microenvironment supports the establishment of breast tumor metastases
Authors: C Chute, X Yang, K Meyer, N Yang, K O'Neil, I Kasza, K Eliceiri, C Alexander, A Friedl
Breast Cancer Res., 2018;20(1):66.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Mesenchymal stem cells attenuate blood-brain barrier leakage after cerebral ischemia in mice
Authors: Z Cheng, L Wang, M Qu, H Liang, W Li, Y Li, L Deng, Z Zhang, GY Yang
J Neuroinflammation, 2018;15(1):135.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Multicolor quantitative confocal imaging cytometry
Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
Nat. Methods, 2018;15(1):39-46.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
The tyrosine phosphatase SHP2 controls TGF?-induced STAT3 signaling to regulate fibroblast activation and fibrosis
Authors: A Zehender, J Huang, AH Györfi, AE Matei, T Trinh-Minh, X Xu, YN Li, CW Chen, J Lin, C Dees, C Beyer, K Gelse, ZY Zhang, C Bergmann, A Ramming, W Birchmeier, O Distler, G Schett, JHW Distler
Nat Commun, 2018;9(1):3259.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Pentoxifylline decreases post-operative intra-abdominal adhesion formation in an animal model
Authors: YL Yang, MG Lee, CC Lee, PI Su, CY Chi, CH Liu, MC Wu, ZS Yen, SC Chen
PeerJ, 2018;6(0):e5434.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Attenuation of Retinal Vascular Development in Neonatal Mice Subjected to Hypoxic-Ischemic Encephalopathy
Authors: IS Zaitoun, U Cikla, D Zafer, E Udho, R Almomani, A Suscha, P Cengiz, CM Sorenson, N Sheibani
Sci Rep, 2018;0(1):9166.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Mesenchymal stem cells ameliorate hyperglycemia-induced endothelial injury through modulation of mitophagy
Authors: W Zhu, Y Yuan, G Liao, L Li, J Liu, Y Chen, J Zhang, J Cheng, Y Lu
Cell Death Dis, 2018;9(8):837.
Species: Rat
Sample Types: Whole Cells
Applications: Flow Cytometry -
KSHV-induced ligand mediated activation of PDGF receptor-alpha drives Kaposi's sarcomagenesis
Authors: LE Cavallin, Q Ma, J Naipauer, S Gupta, M Kurian, P Locatelli, P Romanelli, M Nadji, PJ Goldschmid, EA Mesri
PLoS Pathog., 2018;14(7):e1007175.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Gli1(+) Mesenchymal Stromal Cells Are a Key Driver of Bone Marrow Fibrosis and an Important Cellular Therapeutic Target
Authors: RK Schneider, A Mullally, A Dugourd, F Peisker, R Hoogenboez, PMH Van Strien, EM Bindels, D Heckl, G Büsche, D Fleck, G Müller-New, J Wongboonsi, M Ventura Fe, VG Puelles, J Saez-Rodri, BL Ebert, BD Humphreys, R Kramann
Cell Stem Cell, 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Coronary angiogenic effect of long-term administration of Nigella sativa
Authors: LI Al Asoom
BMC Complement Altern Med, 2017;17(1):308.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Natural compound bavachalcone promotes the differentiation of endothelial progenitor cells and neovascularization through the ROR?-erythropoietin-AMPK axis
Authors: S Ling, RZ Ni, Y Yuan, YQ Dang, QM Zhou, S Liang, F Guo, W Feng, Y Chen, K Ikeda, Y Yamori, JW Xu
Oncotarget, 2017;8(49):86188-86205.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-P -
Activation of Hypoxia Signaling in Stromal Progenitors Impairs Kidney Development
Authors: K Gerl, D Steppan, M Fuchs, C Wagner, C Willam, A Kurtz, B Kurt
Am. J. Pathol., 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Clearance of Heparan Sulfate and Attenuation of CNS Pathology by Intracerebroventricular BMN 250 in Sanfilippo Type B Mice
Authors: M Aoyagi-Sch, D Crippen-Ha, R Lawrence, J Vincelette, G Yogalingam, H Prill, BK Yip, B Baridon, C Vitelli, A Lee, O Gorostiza, EG Adintori, WC Minto, JL Van Vleet, B Yates, S Rigney, TM Christians, PMN Tiger, MJ Lo, J Holtzinger, PA Fitzpatric, JH LeBowitz, S Bullens, BE Crawford, S Bunting
Mol Ther Methods Clin Dev, 2017;6(0):43-53.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Gene therapy for a mouse model of glucose transporter-1 deficiency syndrome
Authors: S Nakamura, H Osaka, SI Muramatsu, N Takino, M Ito, S Aoki, EF Jimbo, K Shimazaki, T Onaka, S Ohtsuki, T Terasaki, T Yamagata
Mol Genet Metab Rep, 2017;10(0):67-74.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Resident Arterial Cells and Circulating Bone Marrow-Derived Cells both Contribute to Intimal Hyperplasia in a Rat Allograft Carotid Transplantation Model
Authors: Y He, X Xu, T Zhu, M Tang, J Mei, Y Si
Cell. Physiol. Biochem., 2017;42(4):1303-1312.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Pericyte-targeting prodrug overcomes tumor resistance to vascular disrupting agents
Authors: M Chen, X Lei, C Shi, M Huang, X Li, B Wu, Z Li, W Han, B Du, J Hu, Q Nie, W Mai, N Ma, N Xu, X Zhang, C Fan, A Hong, M Xia, L Luo, A Ma, H Li, Q Yu, H Chen, D Zhang, W Ye
J. Clin. Invest., 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Brachyury drives formation of a distinct vascular branchpoint critical for fetal-placental arterial union in the mouse gastrula
Authors: AM Rodriguez, DX Jin, AD Wolfe, MM Mikedis, L Wierenga, MP Hashmi, C Viebahn, KM Downs
Dev. Biol., 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Excessive dietary intake of vitamin A reduces skull bone thickness in mice
Authors: T Lind, C Öhman, G Calounova, A Rasmusson, G Andersson, G Pejler, H Melhus
PLoS ONE, 2017;12(4):e0176217.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-P -
Immunofluorescence reveals unusual patterns of labelling for connexin43 localized to calbindin-D28K-positive interstitial cells in the pineal gland
Authors: DD Tsao, SG Wang, BD Lynn, JI Nagy
Eur. J. Neurosci., 2017;0(0):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Endoglin controls blood vessel diameter through endothelial cell shape changes in response to haemodynamic cues
Authors: WW Sugden, R Meissner, T Aegerter-W, R Tsaryk, EV Leonard, J Bussmann, MJ Hamm, W Herzog, Y Jin, L Jakobsson, C Denz, AF Siekmann
Nat. Cell Biol., 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Endoglin prevents vascular malformation by regulating flow-induced cell migration and specification through VEGFR2 signalling
Authors: Y Jin, L Muhl, M Burmakin, Y Wang, AC Duchez, C Betsholtz, HM Arthur, L Jakobsson
Nat. Cell Biol., 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Silencing of galectin-1 inhibits retinal neovascularization and ameliorates retinal hypoxia in a murine model of oxygen-induced ischemic retinopathy
Authors: N Yang, W Zhang, T He, Y Xing
Exp. Eye Res, 2017;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Impacts of femoral artery and vein excision versus femoral artery excision on the hindlimb ischemic model in CD-1 mice
Authors: Miao Chen
Microvasc. Res, 2016;110(0):48-55.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Murine liver-resident group 1 innate lymphoid cells regulate optimal priming of anti-viral CD8+ T cells
J Leukoc Biol, 2016;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
TLR2-mediated leukocyte trafficking to the developing brain
J Leukoc Biol, 2016;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
The transcription factor Mesp1 interacts with cAMP-responsive element binding protein 1 (Creb1) and coactivates Ets variant 2 (Etv2) gene expression.
Authors: Shi X, Zirbes K, Rasmussen T, Ferdous A, Garry M, Koyano-Nakagawa N, Garry D
J Biol Chem, 2015;290(15):9614-25.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
The formyl peptide receptor 1 exerts a tumor suppressor function in human gastric cancer by inhibiting angiogenesis.
Authors: Prevete N, Liotti F, Visciano C, Marone G, Melillo R, de Paulis A
Oncogene, 2015;34(29):3826-38.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Integrin beta1 controls VE-cadherin localization and blood vessel stability.
Authors: Yamamoto H, Ehling M, Kato K, Kanai K, van Lessen M, Frye M, Zeuschner D, Nakayama M, Vestweber D, Adams R
Nat Commun, 2015;6(0):6429.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
MicroRNA-210 overexpression induces angiogenesis and neurogenesis in the normal adult mouse brain.
Authors: Zeng, L, He, X, Wang, Y, Tang, Y, Zheng, C, Cai, H, Liu, J, Wang, Y, Fu, Y, Yang, G-Y
Gene Ther, 2014;21(1):37-43.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
ITE and TCDD differentially regulate the vascular remodeling of rat placenta via the activation of AhR.
Authors: Wu Y, Chen X, Zhou Q, He Q, Kang J, Zheng J, Wang K, Duan T
PLoS ONE, 2014;9(1):e86549.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-P -
Anticancer effects of gemcitabine are enhanced by co-administered iRGD peptide in murine pancreatic cancer models that overexpressed neuropilin-1.
Authors: Akashi Y, Oda T, Ohara Y, Miyamoto R, Kurokawa T, Hashimoto S, Enomoto T, Yamada K, Satake M, Ohkohchi N
Br J Cancer, 2014;110(6):1481-7.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Periadventitial application of rapamycin-loaded nanoparticles produces sustained inhibition of vascular restenosis.
Authors: Shi, Xudong, Chen, Guojun, Guo, Lian-Wan, Si, Yi, Zhu, Men, Pilla, Srikanth, Liu, Bo, Gong, Shaoqin, Kent, K Craig
PLoS ONE, 2014;9(2):e89227.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Characterization of the adverse effects of nicotine on placental development: in vivo and in vitro studies.
Authors: Holloway, A C, Salomon, A, Soares, M J, Garnier, V, Raha, S, Sergent, F, Nicholson, C J, Feige, J J, Benharouga, M, Alfaidy, N
Am J Physiol Endocrinol Metab, 2014;306(4):E443-56.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Peroxisome proliferator-activated receptor alpha protects capillary pericytes in the retina.
Authors: Ding L, Cheng R, Hu Y, Takahashi Y, Jenkins A, Keech A, Humphries K, Gu X, Elliott M, Xia X, Ma J
Am J Pathol, 2014;184(10):2709-20.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Formation of fenestrae in murine liver sinusoids depends on plasmalemma vesicle-associated protein and is required for lipoprotein passage.
Authors: Herrnberger, Leonie, Hennig, Robert, Kremer, Werner, Hellerbrand, Claus, Goepferich, Achim, Kalbitzer, Hans Rob, Tamm, Ernst R
PLoS ONE, 2014;9(12):e115005.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Melanocyte-secreted fibromodulin promotes an angiogenic microenvironment.
Authors: Adini, Irit, Ghosh, Kaustabh, Adini, Avner, Chi, Zai-Long, Yoshimura, Takeru, Benny, Ofra, Connor, Kip M, Rogers, Michael, Bazinet, Lauren, Birsner, Amy E, Bielenberg, Diane R, D'Amato, Robert J
J Clin Invest, 2014;124(1):425-36.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
TGF-beta mediates homing of bone marrow-derived human mesenchymal stem cells to glioma stem cells.
Authors: Shinojima N, Hossain A, Takezaki T, Fueyo J, Gumin J, Gao F, Nwajei F, Marini F, Andreeff M, Kuratsu J, Lang F
Cancer Res, 2013;73(7):2333-44.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Endoglin regulates the activation and quiescence of endothelium by participating in canonical and non-canonical TGF-beta signaling pathways.
Authors: Park S, Dimaio T, Liu W, Wang S, Sorenson C, Sheibani N
J Cell Sci, 2013;126(0):1392-405.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
The critical role of agrin in the hematopoietic stem cell niche.
Authors: Mazzon C, Anselmo A, Cibella J, Soldani C, Destro A, Kim N, Roncalli M, Burden SJ, Dustin ML, Sarukhan A, Viola A
Blood, 2011;118(10):2733-42.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Dysfunctional microvasculature as a consequence of shb gene inactivation causes impaired tumor growth.
Authors: Funa NS, Kriz V, Zang G, Calounova G, Akerblom B, Mares J, Larsson E, Sun Y, Betsholtz C, Welsh M
Cancer Res., 2009;69(5):2141-8.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Crosstalk between keratinocytes and adaptive immune cells in an IkappaBalpha protein-mediated inflammatory disease of the skin.
Authors: Rebholz B, Haase I, Eckelt B, Paxian S, Flaig MJ, Ghoreschi K, Nedospasov SA, Mailhammer R, Debey-Pascher S, Schultze JL, Weindl G, Forster I, Huss R, Stratis A, Ruzicka T, Rocken M, Pfeffer K, Schmid RM, Rupec RA
Immunity, 2007;27(2):296-307.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsCell and Tissue Staining Kits
Immunohistochemistry Reagents
Isotype Controls
Reconstitution Buffers
Secondary Antibodies
Reviews for Mouse/Rat CD31/PECAM-1 Antibody
Average Rating: 4.9 (Based on 11 Reviews)
Have you used Mouse/Rat CD31/PECAM-1 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
Detection of CD31/PECAM‑1 in Mouse brown adipose tissue. 4% PFA fixed Brown adipose tissue was embedded in OCT and cryosectioned at 10µm. After permeabilization and blocking, tissue was incubated overnight at 4˚C with 2µg/ml of gt@CD31 (AF3628) and detected with Alexa fluor dk@gt 488 (1:1000) the next day. CD31 is presented in pseudo color violet here. This product is great, always very clear and bright. I often use it with 488 channel in tissues with strong autofluorescence such as hearts and quadriceps.
One of the best antibodies.
Used on E9.5-E12.5 cryo embedded mouse embryo sections (4% PFA fixed).
Attached picture is E12.5 embryo section.
Dilution used - 1:200
FFPE rat liver stained using AF3628 at 15 µg/mL overnight at 4C. Epitope retrieval was performed at 95 C for 15 minutes in citrate buffer pH 6.0. Secondary was donkey anti-goat Alexa Fluor 555 (psuedocolored red).
Optically cleared 1 mm mouse kidney section
Imaged by diSPIM in 60% TDE with 17X lens
Secondary antibody is donkey anti goat alexa 488
Red is Tubulin Beta 3 stained by alexa 568
Excellent!
FFPE mouse liver stained O/N at 4C with AF3826 at 10 ug/mL in 0.3% PBS-Tx. Secondary Donkey anti-goat Alexa Fluor 488 was applied for 1 hr RT at 2 ug/mL in PBS-Tx. Citrate buffer pH 6.0 used for epitope retrieval.
WD [1:500], HIER with DIVA; secondary ab RTU Polymer-HRP