Mouse/Rat CD31/PECAM-1 Antibody

  (38 citations)
(5 Reviews)
    
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Product Details
Citations (38)
FAQs
Supplemental Products
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  • Species Reactivity
    Mouse, Rat
  • Specificity
    Detects mouse CD31/PECAM-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human CD31 and recombinant porcine CD31 is observed. Detects mouse CD31 and rat CD31 in flow cytometry.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CD31/PECAM-1
    Glu18-Lys590
    Accession # Q08481
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse CD31/PECAM‑1 by Western Blot. Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD31/PECAM‑1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of CD31/PECAM‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Detection of CD31/PECAM‑1 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Immunocytochemistry
CD31/PECAM‑1 in bEnd.3 Mouse Cell Line. CD31/PECAM‑1 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
CD31/PECAM‑1 in Mouse Embryo. CD31/PECAM‑1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to developing endothelium. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
CD31/PECAM‑1 in Mouse Embryo. CD31/PECAM‑1 was detected in immersion fixed frozen sections of mouse embryo (14 d.p.c.) using Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing guts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD31/PECAM-1

PECAM-1 (Platelet-Endothelial Cell Adhesion Molecule-1), also known as CD31, is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily (1, 2). Expression is restricted to cells involved in circulation, especially endothelial cells, platelets, monocytes, neutrophils and lymphocyte subsets. PECAM-1 is concentrated at cell-cell junctions and is required for Transendothelial Migration (TEM) (1-3). The Extracellular Domain (ECD) of PECAM-1 has ten potential N-linked glycosylation sites and six C2-type Ig-like domains, the first of which is critical for adhesion and extravasation (3, 4). The cytoplasmic domain contains Immunoregulatory Tyrosine-based Inhibitory and Switch Motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine-mediated engagement of SH2-containing signaling molecules (1, 5). Metalloproteinase-mediated ectodomain shedding occurs during apoptosis (6) but increased serum PECAM-1 ectodomain in HIV and active multiple sclerosis occurs independent of apoptosis (7, 8). In humans, expression of six isoforms with exon deletions in the cytoplasmic domain is tissue- and stage-specific, but full-length PECAM-1 is predominant. A form lacking the ITSM predominates in mouse (9). Mouse PECAM-1 ECD shows 77%, 63%, 63%, 63%, and 61% amino acid (aa) identity with rat, human, canine, porcine, and bovine PECAM-1, respectively. PECAM-1 participates with other adhesion molecules in some functions, but is the critical molecule for TEM. Homotypic PECAM-1 adhesion in trans, combined with cycling of PECAM-1 to and from surface-connected endothelial cell vesicles, leads leukocytes across endothelial tight junctions (3, 10). Homotypic adhesion and signaling functions also strongly suppress mitochondria-dependent apoptosis (11). In platelets, PECAM-1 is necessary for limiting thrombus formation (12) and promoting integrin-mediated clot retraction and platelet spreading (13), but mechanisms for these phenomena are unclear. PECAM-/- mice are deficient in chemokine-mediated chemotaxis (14).

  • References:
    1. Ilan, N. and J.A. Madri (2003) Curr. Opin. Cell Biol. 15:515.
    2. Xie, Y. and W.A. Muller (1993) Proc. Natl. Acad. Sci. USA 90:5569.
    3. Liao, F. et al. (1997) J. Exp. Med. 185:1349.
    4. Nakada, M.T. et al. (2000) J. Immunol. 164:452.
    5. Chemnitz, J.M. et al. (2004) J. Immunol. 173:945.
    6. Ilan, N. et al. (2001) FASEB J. 15:362.
    7. Eugenin, E.A. et al. (2006) J. Leukoc. Biol. 79:444.
    8. Losy, J. et al. (1999) J. Neuroimmunol. 99:169.
    9. Wang, Y. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284:H1008.
    10. Mamdouh, Z. et al. (2003) Nature 421:748.
    11. Gao, C. et al. (2003) Blood 102:169.
    12. Falati, S. et al. (2006) Blood 107:535.
    13. Wee, J.L. and D.E. Jackson (2005) Blood 106:3816.
    14. Wu, Y. et al. (2005) J. Immunol. 175:3484.
  • Long Name:
    Platelet Endothelial Cell Adhesion Molecule 1
  • Entrez Gene IDs:
    5175 (Human); 18613 (Mouse); 29583 (Rat)
  • Alternate Names:
    adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; endoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

38 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Dosimetric evaluation of radionuclides for VCAM-1-targeted radionuclide therapy of early brain metastases
    Authors: N Falzone, NL Ackerman, LF Rosales, MA Bernal, X Liu, SG Peeters, MS Soto, A Corroyer-D, M Bernaudin, E Grimoin, O Touzani, NR Sibson, KA Vallis
    Theranostics, 2018;8(1):292-303.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Basal lamina remodeling at the skeletal muscle stem cell niche mediates stem cell self-renewal
    Authors: SS Rayagiri, D Ranaldi, A Raven, NIF Mohamad Az, O Lefebvre, PS Zammit, AG Borycki
    Nat Commun, 2018;9(1):1075.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  3. Local delivery of tetramethylpyrazine eliminates the senescent phenotype of bone marrow mesenchymal stromal cells and creates an anti-inflammatory and angiogenic environment in aging mice
    Authors: B Gao, X Lin, H Jing, J Fan, C Ji, Q Jie, C Zheng, D Wang, X Xu, Y Hu, W Lu, Z Luo, L Yang
    Aging Cell, 2018;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. UHRF1 epigenetically orchestrates smooth muscle cell plasticity in arterial disease
    Authors: L Elia, P Kunderfran, P Carullo, M Vacchiano, FM Farina, IF Hall, S Mantero, C Panico, R Papait, G Condorelli, M Quintavall
    J. Clin. Invest., 2018;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  5. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2018;15(1):39-46.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  6. Hindlimb Ischemia Impairs Endothelial Recovery and Increases Neointimal Proliferation in the Carotid Artery
    Authors: S Sorrentino, C Iaconetti, S De Rosa, A Polimeni, J Sabatino, C Gareri, F Passafaro, T Mancuso, L Tammè, C Mignogna, C Camastra, G Esposito, A Curcio, D Torella, C Indolfi
    Sci Rep, 2018;8(1):761.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC-P
  7. Immunofluorescence reveals unusual patterns of labelling for connexin43 localized to calbindin-D28K-positive interstitial cells in the pineal gland
    Authors: DD Tsao, SG Wang, BD Lynn, JI Nagy
    Eur. J. Neurosci., 2017;0(0):.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC-F
  8. Resident Arterial Cells and Circulating Bone Marrow-Derived Cells both Contribute to Intimal Hyperplasia in a Rat Allograft Carotid Transplantation Model
    Authors: Y He, X Xu, T Zhu, M Tang, J Mei, Y Si
    Cell. Physiol. Biochem., 2017;42(4):1303-1312.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  9. Endoglin prevents vascular malformation by regulating flow-induced cell migration and specification through VEGFR2 signalling
    Authors: Y Jin, L Muhl, M Burmakin, Y Wang, AC Duchez, C Betsholtz, HM Arthur, L Jakobsson
    Nat. Cell Biol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  10. Clearance of Heparan Sulfate and Attenuation of CNS Pathology by Intracerebroventricular BMN 250 in Sanfilippo Type B Mice
    Authors: M Aoyagi-Sch, D Crippen-Ha, R Lawrence, J Vincelette, G Yogalingam, H Prill, BK Yip, B Baridon, C Vitelli, A Lee, O Gorostiza, EG Adintori, WC Minto, JL Van Vleet, B Yates, S Rigney, TM Christians, PMN Tiger, MJ Lo, J Holtzinger, PA Fitzpatric, JH LeBowitz, S Bullens, BE Crawford, S Bunting
    Mol Ther Methods Clin Dev, 2017;6(0):43-53.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  11. Coronary angiogenic effect of long-term administration of Nigella sativa
    Authors: LI Al Asoom
    BMC Complement Altern Med, 2017;17(1):308.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  12. Silencing of galectin-1 inhibits retinal neovascularization and ameliorates retinal hypoxia in a murine model of oxygen-induced ischemic retinopathy
    Authors: N Yang, W Zhang, T He, Y Xing
    Exp. Eye Res, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  13. Pericyte-targeting prodrug overcomes tumor resistance to vascular disrupting agents
    Authors: M Chen, X Lei, C Shi, M Huang, X Li, B Wu, Z Li, W Han, B Du, J Hu, Q Nie, W Mai, N Ma, N Xu, X Zhang, C Fan, A Hong, M Xia, L Luo, A Ma, H Li, Q Yu, H Chen, D Zhang, W Ye
    J. Clin. Invest., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  14. Natural compound bavachalcone promotes the differentiation of endothelial progenitor cells and neovascularization through the ROR?-erythropoietin-AMPK axis
    Authors: S Ling, RZ Ni, Y Yuan, YQ Dang, QM Zhou, S Liang, F Guo, W Feng, Y Chen, K Ikeda, Y Yamori, JW Xu
    Oncotarget, 2017;8(49):86188-86205.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC-P
  15. Gli1(+) Mesenchymal Stromal Cells Are a Key Driver of Bone Marrow Fibrosis and an Important Cellular Therapeutic Target
    Authors: RK Schneider, A Mullally, A Dugourd, F Peisker, R Hoogenboez, PMH Van Strien, EM Bindels, D Heckl, G Büsche, D Fleck, G Müller-New, J Wongboonsi, M Ventura Fe, VG Puelles, J Saez-Rodri, BL Ebert, BD Humphreys, R Kramann
    Cell Stem Cell, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  16. Brachyury drives formation of a distinct vascular branchpoint critical for fetal-placental arterial union in the mouse gastrula
    Authors: AM Rodriguez, DX Jin, AD Wolfe, MM Mikedis, L Wierenga, MP Hashmi, C Viebahn, KM Downs
    Dev. Biol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  17. Endoglin controls blood vessel diameter through endothelial cell shape changes in response to haemodynamic cues
    Authors: WW Sugden, R Meissner, T Aegerter-W, R Tsaryk, EV Leonard, J Bussmann, MJ Hamm, W Herzog, Y Jin, L Jakobsson, C Denz, AF Siekmann
    Nat. Cell Biol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  18. Excessive dietary intake of vitamin A reduces skull bone thickness in mice
    Authors: T Lind, C Öhman, G Calounova, A Rasmusson, G Andersson, G Pejler, H Melhus
    PLoS ONE, 2017;12(4):e0176217.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  19. Gene therapy for a mouse model of glucose transporter-1 deficiency syndrome
    Authors: S Nakamura, H Osaka, SI Muramatsu, N Takino, M Ito, S Aoki, EF Jimbo, K Shimazaki, T Onaka, S Ohtsuki, T Terasaki, T Yamagata
    Mol Genet Metab Rep, 2017;10(0):67-74.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  20. Impacts of femoral artery and vein excision versus femoral artery excision on the hindlimb ischemic model in CD-1 mice
    Authors: Miao Chen
    Microvasc. Res, 2016;110(0):48-55.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  21. Murine liver-resident group 1 innate lymphoid cells regulate optimal priming of anti-viral CD8+ T cells
    J Leukoc Biol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  22. TLR2-mediated leukocyte trafficking to the developing brain
    J Leukoc Biol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  23. Integrin beta1 controls VE-cadherin localization and blood vessel stability.
    Authors: Yamamoto H, Ehling M, Kato K, Kanai K, van Lessen M, Frye M, Zeuschner D, Nakayama M, Vestweber D, Adams R
    Nat Commun, 2015;6(0):6429.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  24. The formyl peptide receptor 1 exerts a tumor suppressor function in human gastric cancer by inhibiting angiogenesis.
    Authors: Prevete N, Liotti F, Visciano C, Marone G, Melillo R, de Paulis A
    Oncogene, 2015;34(29):3826-38.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  25. The transcription factor Mesp1 interacts with cAMP-responsive element binding protein 1 (Creb1) and coactivates Ets variant 2 (Etv2) gene expression.
    Authors: Shi X, Zirbes K, Rasmussen T, Ferdous A, Garry M, Koyano-Nakagawa N, Garry D
    J Biol Chem, 2015;290(15):9614-25.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  26. Formation of fenestrae in murine liver sinusoids depends on plasmalemma vesicle-associated protein and is required for lipoprotein passage.
    Authors: Herrnberger, Leonie, Hennig, Robert, Kremer, Werner, Hellerbrand, Claus, Goepferich, Achim, Kalbitzer, Hans Rob, Tamm, Ernst R
    PLoS ONE, 2014;9(12):e115005.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  27. MicroRNA-210 overexpression induces angiogenesis and neurogenesis in the normal adult mouse brain.
    Authors: Zeng, L, He, X, Wang, Y, Tang, Y, Zheng, C, Cai, H, Liu, J, Wang, Y, Fu, Y, Yang, G-Y
    Gene Ther, 2014;21(1):37-43.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-OCT embedded
  28. Peroxisome proliferator-activated receptor alpha protects capillary pericytes in the retina.
    Authors: Ding L, Cheng R, Hu Y, Takahashi Y, Jenkins A, Keech A, Humphries K, Gu X, Elliott M, Xia X, Ma J
    Am J Pathol, 2014;184(10):2709-20.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IF
  29. Characterization of the adverse effects of nicotine on placental development: in vivo and in vitro studies.
    Authors: Holloway, A C, Salomon, A, Soares, M J, Garnier, V, Raha, S, Sergent, F, Nicholson, C J, Feige, J J, Benharouga, M, Alfaidy, N
    Am J Physiol Endocrinol Metab, 2014;306(4):E443-56.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: WB
  30. Melanocyte-secreted fibromodulin promotes an angiogenic microenvironment.
    Authors: Adini, Irit, Ghosh, Kaustabh, Adini, Avner, Chi, Zai-Long, Yoshimura, Takeru, Benny, Ofra, Connor, Kip M, Rogers, Michael, Bazinet, Lauren, Birsner, Amy E, Bielenberg, Diane R, D'Amato, Robert J
    J Clin Invest, 2014;124(1):425-36.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  31. ITE and TCDD differentially regulate the vascular remodeling of rat placenta via the activation of AhR.
    Authors: Wu Y, Chen X, Zhou Q, He Q, Kang J, Zheng J, Wang K, Duan T
    PLoS ONE, 2014;9(1):e86549.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  32. Periadventitial application of rapamycin-loaded nanoparticles produces sustained inhibition of vascular restenosis.
    Authors: Shi, Xudong, Chen, Guojun, Guo, Lian-Wan, Si, Yi, Zhu, Men, Pilla, Srikanth, Liu, Bo, Gong, Shaoqin, Kent, K Craig
    PLoS ONE, 2014;9(2):e89227.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  33. Anticancer effects of gemcitabine are enhanced by co-administered iRGD peptide in murine pancreatic cancer models that overexpressed neuropilin-1.
    Authors: Akashi Y, Oda T, Ohara Y, Miyamoto R, Kurokawa T, Hashimoto S, Enomoto T, Yamada K, Satake M, Ohkohchi N
    Br J Cancer, 2014;110(6):1481-7.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  34. TGF-beta mediates homing of bone marrow-derived human mesenchymal stem cells to glioma stem cells.
    Authors: Shinojima N, Hossain A, Takezaki T, Fueyo J, Gumin J, Gao F, Nwajei F, Marini F, Andreeff M, Kuratsu J, Lang F
    Cancer Res, 2013;73(7):2333-44.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  35. Endoglin regulates the activation and quiescence of endothelium by participating in canonical and non-canonical TGF-beta signaling pathways.
    Authors: Park S, Dimaio T, Liu W, Wang S, Sorenson C, Sheibani N
    J Cell Sci, 2013;126(0):1392-405.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  36. The critical role of agrin in the hematopoietic stem cell niche.
    Authors: Mazzon C, Anselmo A, Cibella J, Soldani C, Destro A, Kim N, Roncalli M, Burden SJ, Dustin ML, Sarukhan A, Viola A
    Blood, 2011;118(10):2733-42.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  37. Dysfunctional microvasculature as a consequence of shb gene inactivation causes impaired tumor growth.
    Authors: Funa NS, Kriz V, Zang G, Calounova G, Akerblom B, Mares J, Larsson E, Sun Y, Betsholtz C, Welsh M
    Cancer Res., 2009;69(5):2141-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  38. Crosstalk between keratinocytes and adaptive immune cells in an IkappaBalpha protein-mediated inflammatory disease of the skin.
    Authors: Rebholz B, Haase I, Eckelt B, Paxian S, Flaig MJ, Ghoreschi K, Nedospasov SA, Mailhammer R, Debey-Pascher S, Schultze JL, Weindl G, Forster I, Huss R, Stratis A, Ruzicka T, Rocken M, Pfeffer K, Schmid RM, Rupec RA
    Immunity, 2007;27(2):296-307.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
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Average Rating: 5 (Based on 5 reviews)

Have you used Mouse/Rat CD31/PECAM-1 Antibody?

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Read what people are saying who have used Mouse/Rat CD31/PECAM-1 Antibody.

We have 5 review tested in 1 species: Mouse.
We have 5 reviews tested in 3 applications: Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Mouse/Rat CD31/PECAM-1 Antibody AF3628 Array AF3628
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Excellent
 ICC/IF Mouse Anonymous 04/05/2018
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Immunocytochemistry/Immunofluorescence Mouse/Rat CD31/PECAM-1 Antibody AF3628
Immunocytochemistry/Immunofluorescence: Mouse/Rat CD31/PECAM-1 Antibody [AF3628].

Summary

Application Immunocytochemistry/Immunofluorescence
Sample Tested Adult brain
Species Mouse

Other Experimental Details

Other Experimental Details Excellent!
Immunohistochemistry Mouse/Rat CD31/PECAM-1 Antibody AF3628 Array AF3628
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Excellent
 IHC Mouse Anonymous 03/26/2018
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Immunohistochemistry Mouse/Rat CD31/PECAM-1 Antibody AF3628
Immunohistochemistry: Mouse/Rat CD31/PECAM-1 Antibody [AF3628].

Summary

Application Immunohistochemistry
Sample Tested Liver tissue
Species Mouse

Other Experimental Details

Other Experimental Details FFPE mouse liver stained O/N at 4C with AF3826 at 10 ug/mL in 0.3% PBS-Tx. Secondary Donkey anti-goat Alexa Fluor 488 was applied for 1 hr RT at 2 ug/mL in PBS-Tx. Citrate buffer pH 6.0 used for epitope retrieval.
Immunohistochemistry Mouse/Rat CD31/PECAM-1 Antibody AF3628 Array AF3628
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Excellent
 IHC Mouse Anonymous 09/12/2017
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Immunohistochemistry Mouse/Rat CD31/PECAM-1 Antibody AF3628
Immunohistochemistry: Mouse/Rat CD31/PECAM-1 Antibody [AF3628].

Summary

Application Immunohistochemistry
Sample Tested Embryonic kidney
Species Mouse
Immunohistochemistry-Paraffin Mouse/Rat CD31/PECAM-1 Antibody AF3628 Array AF3628
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Excellent
 IHC-P Mouse Anonymous 06/29/2017
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Immunohistochemistry-Paraffin Mouse/Rat CD31/PECAM-1 Antibody AF3628
Immunohistochemistry-Paraffin: Mouse/Rat CD31/PECAM-1 Antibody [AF3628].

Summary

Application Immunohistochemistry-Paraffin
Sample Tested Formalin Fixed Paraffin Embedded Mouse Liver
Species Mouse

Other Experimental Details

Other Experimental Details WD [1:500], HIER with DIVA; secondary ab RTU Polymer-HRP
Immunohistochemistry Mouse/Rat CD31/PECAM-1 Antibody AF3628 Array AF3628
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Excellent
 IHC Mouse Anonymous 08/08/2016
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Immunohistochemistry Mouse/Rat CD31/PECAM-1 Antibody AF3628
Immunohistochemistry: Mouse/Rat CD31/PECAM-1 Antibody [AF3628].

Summary

Application Immunohistochemistry
Sample Tested Embryo tissue
Species Mouse

Other Experimental Details

Other Experimental Details E9.5 mouse embryonic tissue, whole mount staining with 1:100 dilution, Alex Fluor 594 donkey anti-goat with 1:400 dilution.

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