Mouse/Rat Contactin-2/TAG1 Antibody

(6 citations)
(1 Review)
  
  • Species Reactivity
    Mouse, Rat
  • Specificity
    Detects mouse and rat Contactin-2 in direct ELISAs and Western blots. In direct ELISAs, 20% cross-reactivity with recombinant human (rh) Contactin‑2 is observed and 2% cross-reactivity with recombinant mouse (rm) Contactin‑3, rhContactin‑4, rmContactin‑4, rhContactin‑5, and rmContactin‑6 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Contactin-2/TAG1
    Gln31-Ser1014
    Accession # Q61330
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse and Rat Contactin‑2/TAG1 by Western Blot. Western blot shows lysates of mouse spinal cord tissue and rat embryonic cortical neuron/glial cells. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat Contactin‑2/TAG1 Polyclonal Antibody (Catalog # AF4439) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Contactin‑2/TAG1 at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Immunohistochemistry

Contactin‑2/TAG1 in Mouse Embryo. Contactin‑2/TAG1 was detected in immersion fixed frozen sections of mouse embryo (E13) using Goat Anti-Mouse/Rat Contactin‑2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4439) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells in proximity to ribs. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Mouse Contactin‑2/TAG1 by Simple WesternTM. Simple Western lane view shows lysates of mouse spinal cord tissue, loaded at 0.2 mg/mL. A specific band was detected for Contactin‑2/TAG1 at approximately 162 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse/Rat Contactin‑2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4439) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Contactin-2/TAG1

Contactin-2 (CNTN2), also called TAG1 (transient axonal glycoprotein), TAX1 (transiently-expressed axonal glycoprotein), or axonin-1, is a 135 kDa glycosyl-phosphatidylinositol (GPI)- anchored cell adhesion molecule that belongs to the contactin subfamily within the immunoglobulin (Ig) protein superfamily (1-3). Mouse Contactin-2 cDNA encodes a 30 amino acid (aa) signal peptide, a 984 aa mature secreted protein with 6 Ig-like domains followed by 4 fibronectin type III-like repeats, and a 26 aa C-terminal GPI anchor pro-sequence. GPI-specific phospholipase activity can release soluble, active Contactin-2 from the membrane (2). Mature mouse Contactin-2 shares approximately 93%, 97%, and 77% aa sequence identity with human, rat, and chicken Contactin-2, respectively. During development, Contactin-2 is expressed by a subset of neuronal populations in the central nervous system (CNS) and peripheral nervous system (PNS), particularly during initial phases of axon outgrowth (3-5). Both the 135 kDa form and a 90 kDa form are also upregulated in response to CNS injury in the adult (6). Data support a role for Contactin-2 in axon pathfinding, neurite outgrowth and adhesion, especially in the CNS (3-6). In mature myelinated fibers, Contactin-2 is expressed by oligodendrocytes and Schwann cells, which are myelinating glial cells of the CNS and PNS, respectively (7, 8). It is enriched in the juxtaparanodal regions, where it recruits contactin-associated protein 2 (caspr2), a transmembrane neurexin involved in cell adhesion and intercellular communication (7-10). The axonal Contactin-2 interacts in cis with caspr2 and in trans with another Contactin-2 on the glial membrane (8). This ternary complex is required for the accumulation and organization of K+ channels in the juxtaparanodes (9).

  • References:
    1. Wolfer, D. and R.J. Giger (1994) Swissprot Accession # Q61330.
    2. Hasler, T.H. et al. (1993) Eur. J. Biochem. 211:329.
    3. Karagogeos, D. (2003) Front. Biosci. 8:s1304.
    4. Liu, Y. and M.C. Halloran (2005) J. Neurosci. 25:10556.
    5. Denaxa, M. et al. (2005) Dev. Biol. 288:87.
    6. Soares, S. et al. (2005) Eur. J. Neurosci. 21:1169.
    7. Traka, M. et al. (2002) J. Neurosci. 22:3016.
    8. Poliak, S. and E. Peles (2003) Nat. Rev. Neurosci. 4:968.
    9. Traka, M. et al. (2003) J. Cell Biol. 162:1161.
    10. Poliak, S. et al. (2003) J. Cell Biol. 162:1149.
  • Entrez Gene IDs:
    6900 (Human)
  • Alternate Names:
    Axonal glycoprotein TAG-1; axonin-1 cell adhesion molecule; Axonin-1; AXT; CNTN2; contactin 2 (axonal); contactin 2 (transiently expressed); Contactin2; Contactin-2; DKFZp781D102; FLJ37193; MGC157722; TAG1; TAG-1; TAX; TAX1; TAX-1; TAX1FLJ42746; Transient axonal glycoprotein 1; transiently-expressed axonal glycoprotein
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Sample Type
  1. Phenotypically silent Cre recombination within the postnatal ventricular conduction system
    Authors: S Bhattachar, M Bhakta, NV Munshi
    PLoS ONE, 2017;12(3):e0174517.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  2. Genome Stability by DNA polymerase ? in Neural Progenitors Contributes to Neuronal Differentiation in Cortical Development
    Authors: K Onishi, A Uyeda, M Shida, T Hirayama, T Yagi, N Yamamoto, N Sugo
    J. Neurosci., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  3. NOVA2-mediated RNA regulation is required for axonal pathfinding during development
    Elife, 2016;5(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  4. An aberrant sugar modification of BACE1 blocks its lysosomal targeting in Alzheimer's disease.
    Authors: Kizuka Y, Kitazume S, Fujinawa R, Saito T, Iwata N, Saido T, Nakano M, Yamaguchi Y, Hashimoto Y, Staufenbiel M, Hatsuta H, Murayama S, Manya H, Endo T, Taniguchi N
    EMBO Mol Med, 2015;7(2):175-89.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  5. BACE1 activity regulates cell surface contactin-2 levels.
    Authors: Gautam, Vivek, D'Avanzo, Carla, Hebisch, Matthias, Kovacs, Dora M, Kim, Doo Yeon
    Mol Neurodegener, 2014;9(0):4.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  6. Secretome protein enrichment identifies physiological BACE1 protease substrates in neurons.
    Authors: Kuhn PH, Koroniak K, Hogl S, Colombo A, Zeitschel U, Willem M, Volbracht C, Schepers U, Imhof A, Hoffmeister A, Haass C, Rossner S, Brase S, Lichtenthaler SF
    EMBO J., 2012;31(14):3157-68.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
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Average Rating: 5 (Based on 1 review)

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Western Blot Mouse/Rat Contactin‑2/TAG1 Antibody AF4439
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 WB Mouse Anonymous 04/26/2016
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Western Blot Mouse/Rat Contactin‑2/TAG1 Antibody AF4439
Western Blot: Mouse/Rat Contactin‑2/TAG1 Antibody [AF4439]

Summary

ApplicationWestern Blot
Sample TestedBrain tissue
SpeciesMouse

Other Experimental Details

Other Experimental Details80, 40 and 20ug of total mouse brain homogenate was separated by SDS-PAGE and transfered to PVDF membraneBlock: 1% BSA, 1% FSG PBS-T 1h RTPrimary: 1:1000, 1% BSA, 1% FSG PBS-T O/N 4oCSecondary: 1:5000,1% BSA, 1% FSG PBS-T 2h RT(Secondary - DaG 800, Licor)

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