Mouse/Rat Contactin-2/TAG1 Antibody

Catalog # Availability Size / Price Qty
AF4439
AF4439-SP
Detection of Mouse and Rat Contactin‑2/TAG1 by Western Blot.
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Product Details
Citations (7)
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Reviews (1)

Mouse/Rat Contactin-2/TAG1 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat Contactin-2 in direct ELISAs and Western blots. In direct ELISAs, 20% cross-reactivity with recombinant human (rh) Contactin‑2 is observed and 2% cross-reactivity with recombinant mouse (rm) Contactin‑3, rhContactin‑4, rmContactin‑4, rhContactin‑5, and rmContactin‑6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Contactin-2/TAG1
Gln31-Ser1014
Accession # Q61330
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse and Rat Contactin‑2/TAG1 by Western Blot. View Larger

Detection of Mouse and Rat Contactin‑2/TAG1 by Western Blot. Western blot shows lysates of mouse spinal cord tissue and rat embryonic cortical neuron/glial cells. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat Contactin‑2/TAG1 Polyclonal Antibody (Catalog # AF4439) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Contactin‑2/TAG1 at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry Contactin‑2/TAG1 in Mouse Embryo. View Larger

Contactin‑2/TAG1 in Mouse Embryo. Contactin‑2/TAG1 was detected in immersion fixed frozen sections of mouse embryo (E13) using Goat Anti-Mouse/Rat Contactin‑2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4439) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells in proximity to ribs. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western Detection of Mouse Contactin‑2/TAG1 by Simple WesternTM. View Larger

Detection of Mouse Contactin‑2/TAG1 by Simple WesternTM. Simple Western lane view shows lysates of mouse spinal cord tissue, loaded at 0.2 mg/mL. A specific band was detected for Contactin‑2/TAG1 at approximately 162 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse/Rat Contactin‑2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4439) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Contactin-2/TAG1

Contactin-2 (CNTN2), also called TAG1 (transient axonal glycoprotein), TAX1 (transiently-expressed axonal glycoprotein), or axonin-1, is a 135 kDa glycosyl-phosphatidylinositol (GPI)- anchored cell adhesion molecule that belongs to the contactin subfamily within the immunoglobulin (Ig) protein superfamily (1-3). Mouse Contactin-2 cDNA encodes a 30 amino acid (aa) signal peptide, a 984 aa mature secreted protein with 6 Ig-like domains followed by 4 fibronectin type III-like repeats, and a 26 aa C-terminal GPI anchor pro-sequence. GPI-specific phospholipase activity can release soluble, active Contactin-2 from the membrane (2). Mature mouse Contactin-2 shares approximately 93%, 97%, and 77% aa sequence identity with human, rat, and chicken Contactin-2, respectively. During development, Contactin-2 is expressed by a subset of neuronal populations in the central nervous system (CNS) and peripheral nervous system (PNS), particularly during initial phases of axon outgrowth (3-5). Both the 135 kDa form and a 90 kDa form are also upregulated in response to CNS injury in the adult (6). Data support a role for Contactin-2 in axon pathfinding, neurite outgrowth and adhesion, especially in the CNS (3-6). In mature myelinated fibers, Contactin-2 is expressed by oligodendrocytes and Schwann cells, which are myelinating glial cells of the CNS and PNS, respectively (7, 8). It is enriched in the juxtaparanodal regions, where it recruits contactin-associated protein 2 (caspr2), a transmembrane neurexin involved in cell adhesion and intercellular communication (7-10). The axonal Contactin-2 interacts in cis with caspr2 and in trans with another Contactin-2 on the glial membrane (8). This ternary complex is required for the accumulation and organization of K+ channels in the juxtaparanodes (9).

References
  1. Wolfer, D. and R.J. Giger (1994) Swissprot Accession # Q61330.
  2. Hasler, T.H. et al. (1993) Eur. J. Biochem. 211:329.
  3. Karagogeos, D. (2003) Front. Biosci. 8:s1304.
  4. Liu, Y. and M.C. Halloran (2005) J. Neurosci. 25:10556.
  5. Denaxa, M. et al. (2005) Dev. Biol. 288:87.
  6. Soares, S. et al. (2005) Eur. J. Neurosci. 21:1169.
  7. Traka, M. et al. (2002) J. Neurosci. 22:3016.
  8. Poliak, S. and E. Peles (2003) Nat. Rev. Neurosci. 4:968.
  9. Traka, M. et al. (2003) J. Cell Biol. 162:1161.
  10. Poliak, S. et al. (2003) J. Cell Biol. 162:1149.
Entrez Gene IDs
6900 (Human)
Alternate Names
Axonal glycoprotein TAG-1; axonin-1 cell adhesion molecule; Axonin-1; AXT; CNTN2; contactin 2 (axonal); contactin 2 (transiently expressed); Contactin2; Contactin-2; DKFZp781D102; FLJ37193; MGC157722; TAG1; TAG-1; TAX; TAX1; TAX-1; TAX1FLJ42746; Transient axonal glycoprotein 1; transiently-expressed axonal glycoprotein

Product Datasheets

Citations for Mouse/Rat Contactin-2/TAG1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Genome Stability by DNA polymerase ? in Neural Progenitors Contributes to Neuronal Differentiation in Cortical Development
    Authors: K Onishi, A Uyeda, M Shida, T Hirayama, T Yagi, N Yamamoto, N Sugo
    J. Neurosci., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. MUNC18-1 gene abnormalities are involved in neurodevelopmental disorders through defective cortical architecture during brain development
    Authors: N Hamada, I Iwamoto, H Tabata, KI Nagata
    Acta Neuropathol Commun, 2017;5(1):92.
    Species: Primate
    Sample Types: Whole Cells
    Applications: ICC
  3. Phenotypically silent Cre recombination within the postnatal ventricular conduction system
    Authors: S Bhattachar, M Bhakta, NV Munshi
    PLoS ONE, 2017;12(3):e0174517.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  4. NOVA2-mediated RNA regulation is required for axonal pathfinding during development
    Elife, 2016;5(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC OCT-embedded
  5. An aberrant sugar modification of BACE1 blocks its lysosomal targeting in Alzheimer's disease.
    Authors: Kizuka Y, Kitazume S, Fujinawa R, Saito T, Iwata N, Saido T, Nakano M, Yamaguchi Y, Hashimoto Y, Staufenbiel M, Hatsuta H, Murayama S, Manya H, Endo T, Taniguchi N
    EMBO Mol Med, 2015;7(2):175-89.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  6. BACE1 activity regulates cell surface contactin-2 levels.
    Authors: Gautam, Vivek, D'Avanzo, Carla, Hebisch, Matthias, Kovacs, Dora M, Kim, Doo Yeon
    Mol Neurodegener, 2014;9(0):4.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  7. Secretome protein enrichment identifies physiological BACE1 protease substrates in neurons.
    Authors: Kuhn PH, Koroniak K, Hogl S, Colombo A, Zeitschel U, Willem M, Volbracht C, Schepers U, Imhof A, Hoffmeister A, Haass C, Rossner S, Brase S, Lichtenthaler SF
    EMBO J., 2012;31(14):3157-68.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB

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Mouse/Rat Contactin‑2/TAG1 Antibody
By Anonymous on 04/26/2016
Application: WB Sample Tested: Brain tissue Species: Mouse

80, 40 and 20ug of total mouse brain homogenate was separated by SDS-PAGE and transfered to PVDF membraneBlock: 1% BSA, 1% FSG PBS-T 1h RTPrimary: 1:1000, 1% BSA, 1% FSG PBS-T O/N 4oCSecondary: 1:5000,1% BSA, 1% FSG PBS-T 2h RT(Secondary - DaG 800, Licor)