Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Rat

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TRH-degrading Ectoenzyme/TRHDE Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TRH-degrading Ectoenzyme/TRHDE
Arg64-His1025
Accession # Q8K093

Specificity

Detects mouse and rat TRH-degrading Ectoenzyme/TRHDE in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse/Rat TRH-degrading Ectoenzyme/TRHDE Antibody

Detection of Mouse and Rat TRH-degrading Ectoenzyme/ TRHDE antibody by Western Blot.

Detection of Mouse and Rat TRH-degrading Ectoenzyme/ TRHDE by Western Blot.

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and embryonic rat brain hippocampal glial. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat TRH-degrading Ectoenzyme/TRHDE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2985) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TRH-degrading Ectoenzyme/TRHDE at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Mouse/Rat TRH-degrading Ectoenzyme/TRHDE Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: SH‑SY5Y human neuroblastoma cell line and embryonic rat brain hippocampal glial

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRH-degrading Ectoenzyme/TRHDE

TRHDE, also known as pyroglutamyl peptidase II and thyroliberinase, is a metalloprotease that specifically removes pyroglutamate from thyrotropin-releasing hormone, a tripeptide of L-pyroglutamyl-L-histidyl-L-prolineamide. TRH functions as a hypothalamic hypophysiotropic neuropeptide and neurotransmitter/neuromodulator within the central nervous system (1). Inhibitors of TRHDE have potential applications as research and therapeutic agents because TRHDE inactivates TRH (2). TRHDE is a type II transmembrane protein and a soluble form is also present in the serum (1). The recombinant mouse TRHDE corresponds to the ectodomain of the enzyme. Its amino acid sequence is 97% and 95% identical to that of rat and human.

References

  1. Schmitmeier, S. et al. (2002) Eur. J. Biochem. 269:1278.
  2. Kelly, J.A. et al. (2005) Biochem. J. 389:569.

Long Name

Thyrotropin Releasing Hormone-degrading Ectoenzyme

Alternate Names

PAP-II, PGPEP2, Thyroliberinase, TRH-DE, TRHDE, TRHdegrading Ectoenzyme

Entrez Gene IDs

29953 (Human); 237553 (Mouse)

Gene Symbol

TRHDE

UniProt

Q8K093

Additional TRH-degrading Ectoenzyme/TRHDE Products

Product Documents for Mouse/Rat TRH-degrading Ectoenzyme/TRHDE Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse/Rat TRH-degrading Ectoenzyme/TRHDE Antibody

For research use only

Citations for Mouse/Rat TRH-degrading Ectoenzyme/TRHDE Antibody

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