Mouse Semaphorin 6C Antibody

Catalog # Availability Size / Price Qty
AF2108
AF2108-SP
Semaphorin 6C in Mouse Embryo.
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Product Details
Citations (1)
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Mouse Semaphorin 6C Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Semaphorin 6C in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) Semaphorin 6B and rhSemaphorin 6D is observed, 5% cross-reactivity with rhSemaphorin 6A is observed, and less than 3% cross‑reactivity with rhSemaphorin 3A, rhSemaphorin 3B, recombinant mouse (rm) Semaphorin 3C, rmSemaphorin 3E, rmSemaphorin 3F, and rmSemaphorin 7A is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 6C
Ala26-Ile602
Accession # Q9WTM3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Semaphorin 6C (Catalog # 2108-S6)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Semaphorin 6C in Mouse Embryo. View Larger

Semaphorin 6C in Mouse Embryo. Semaphorin 6C was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c) using Goat Anti-Mouse Semaphorin 6C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2108) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of neuronal cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 6C

Semaphorin 6C (Sema6C; previously Sema Y) is a 120 kDa member of the Semaphorin family of axon guidance molecules (1-3). The four known Class 6 semaphorins are type I transmembrane glycoproteins that are most like Class 1 invertebrate semaphorins in structure, and exhibit neuropilin-independent binding to specific plexin A receptors (1-3). Amino acid (aa) identity of Class 6 semaphorins is around 40% overall, but 53-64% within the Sema domain. Sema6C is expressed developmentally in subregions of the central and peripheral nervous systems, heart, and kidney, and primarily in skeletal muscle in adults (3, 4). Mouse Sema6C cDNA encodes 931 aa, including a 25 aa signal sequence, a 580 aa extracellular domain (ECD) including the Sema domain, a 21 aa transmembrane sequence and a 305 aa cytoplasmic portion. Alternate exon splicing creates a 923 aa short form (Sema6C.3) that is lacking aa 185-224 within the Sema domain, but contains 32 unique aa inserted at aa 586; postnatally, this form predominates in muscle (2, 3). The long form predominates in brain, especially in areas of increased plasticity (4). Mouse Sema6C ECD shares 98%, 92%, 92%, 92%, and 88% aa sequence identity with corresponding rat, human, porcine, equine, and canine sequences, respectively. Sema6C, along with Sema6D, is co-expressed with and binds to Plexin A1 (5). This interaction is thought to guide proprioceptive peripheral neurons by repulsion, excluding them from the superficial dorsal horn of the spinal cord (5). Sema6C is downregulated and redistributed following denervation or axotomy, potentially promoting regrowth (4, 6). In muscle, Sema6C is concentrated at neuromuscular junctions (6).

References
  1. Zhou, Y. et al. (2008) Trends Biol. Sci. 33:161.
  2. Qu, X. et al. (2002) J. Biol. Chem. 277:35574.
  3. Kikuchi, K. et al. (1999) Mol. Cell. Neurosci. 13:9.
  4. Burgaya, F. et al. (2006) Mol. Cell. Neurosci. 33:321.
  5. Yoshida, Y. et al. (2006) Neuron 52:775.
  6. Svensson, A. et al. (2008) J. Mol. Hist. 39:5.
Entrez Gene IDs
10500 (Human); 20360 (Mouse)
Alternate Names
KIAA1869; m-SemaY; m-SemaY2; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6C; Sema Y; Sema6C; Semaphorin 6C; Semaphorin Y; semaphorin-6C; semaphorin-Y; SEMAY

Product Datasheets

Citation for Mouse Semaphorin 6C Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Low expression of SEMA6C accelerates the primordial follicle activation in the neonatal mouse ovary
    Authors: S Zhou, W Yan, W Shen, J Cheng, Y Xi, S Yuan, F Fu, T Ding, A Luo, S Wang
    J. Cell. Mol. Med., 2018;22(1):486-496.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB

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