Mouse Serpin F2/ alpha 2-Antiplasmin Antibody

Catalog # Availability Size / Price Qty
AF1239
AF1239-SP
Serpin F2/ alpha 2‑Antiplasmin in Mouse Intestine.
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Citations (1)
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Mouse Serpin F2/ alpha 2-Antiplasmin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Serpin F2/ alpha 2‑Antiplasmin in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human (rh) Serpin F2 and less than 1% cross-reactivity with recombinant mouse (rm) Serpin C1, rmSerpin D1, rhSerpin A1, rmSerpin A5, and rhSerpin C1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Serpin F2/ alpha 2‑Antiplasmin
Val28-Lys491
Accession # Q61247
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Serpin F2/ alpha 2‑Antiplasmin (Catalog # 1239-PI)
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Serpin F2/ alpha 2‑Antiplasmin (Catalog # 1239-PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Serpin F2/ alpha 2‑Antiplasmin in Mouse Intestine. View Larger

Serpin F2/ alpha 2‑Antiplasmin in Mouse Intestine. Serpin F2/ alpha 2‑Antiplasmin was detected in perfusion fixed frozen sections of mouse intestine using 5 µg/mL Goat Anti-Mouse Serpin F2/ alpha 2‑Antiplasmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1239) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin F2/alpha 2-Antiplasmin

Serpin F2 is a member of the Serpin superfamily and the primary physiological inhibitor of the serine protease plasmin, which is responsible for the dissolution of fibrin clots (1, 2). In addition to plasmin, Serpin F2 is also an efficient inhibitor of trypsin and chymotrypsin (3). Liver and kidney are major sites of Serpin F2 production and other tissues such as muscle, intestine, central nervous system, and placenta also express its mRNA at a moderate level (3). The tissue expression pattern of Serpin F2 indicates that it is a key regulator of plasmin-mediated proteolysis in these tissues. Mouse Serpin F2 is synthesized as a 491 amino acid precursor with a 27 amino acid signal peptide. The secreted protein has a short propeptide (residues 28-39) and a mature chain (residues 40-491). For human Serpin F2, the presence of the propeptide did not affect its ability to inhibit plasmin but reduced its cross-linking ability to fibrin (4).

References
  1. Menoud, P-A. et al. (1996) J. Clin. Invest. 97:2478.
  2. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  3. Potempa, J. et al. (1988) Science. 241:699.
  4. Sumi, Y. et al. (1989) J. Biochem. 106:703.
Entrez Gene IDs
5345 (Human); 18816 (Mouse)
Alternate Names
AAPclade F (alpha-2 antiplasmin; alpha 2-Antiplasmin; Alpha-2-AP; Alpha-2-PI; Alpha-2-plasmin inhibitor; pigment epithelium derived factor), member 2; Serpin F2; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epitheliumderived factor), member 2

Product Datasheets

Citation for Mouse Serpin F2/ alpha 2-Antiplasmin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. alpha2-antiplasmin is associated with the progression of fibrosis.
    Authors: Kanno Y, Kawashita E, Minamida M, Kaneiwa A, Okada K, Ueshima S, Matsuo O, Matsuno H
    Am. J. Pathol., 2010;176(1):238-45.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB

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Cell and Tissue Staining Kits

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Immunohistochemistry Reagents

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Secondary Antibodies

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