Mouse Serpin F2/ alpha 2-Antiplasmin Antibody Summary
Accession # Q61247
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Serpin F2/ alpha 2‑Antiplasmin in Mouse Intestine. Serpin F2/a2‑Antiplasmin was detected in perfusion fixed frozen sections of mouse intestine using 5 µg/mL Goat Anti-Mouse Serpin F2/a2‑Antiplasmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1239) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin F2/alpha 2-Antiplasmin
Serpin F2 is a member of the Serpin superfamily and the primary physiological inhibitor of the serine protease plasmin, which is responsible for the dissolution of fibrin clots (1, 2). In addition to plasmin, Serpin F2 is also an efficient inhibitor of trypsin and chymotrypsin (3). Liver and kidney are major sites of Serpin F2 production and other tissues such as muscle, intestine, central nervous system, and placenta also express its mRNA at a moderate level (3). The tissue expression pattern of Serpin F2 indicates that it is a key regulator of plasmin-mediated proteolysis in these tissues. Mouse Serpin F2 is synthesized as a 491 amino acid precursor with a 27 amino acid signal peptide. The secreted protein has a short propeptide (residues 28-39) and a mature chain (residues 40-491). For human Serpin F2, the presence of the propeptide did not affect its ability to inhibit plasmin but reduced its cross-linking ability to fibrin (4).
- Menoud, P-A. et al. (1996) J. Clin. Invest. 97:2478.
- Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
- Potempa, J. et al. (1988) Science. 241:699.
- Sumi, Y. et al. (1989) J. Biochem. 106:703.
Citation for Mouse Serpin F2/ alpha 2-Antiplasmin Antibody
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1 Citation: Showing 1 - 1
alpha2-antiplasmin is associated with the progression of fibrosis.
Authors: Kanno Y, Kawashita E, Minamida M, Kaneiwa A, Okada K, Ueshima S, Matsuo O, Matsuno H
Am. J. Pathol., 2010;176(1):238-45.
Sample Types: Tissue Homogenates
Applications: Western Blot
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