Detects mouse Serpin F2/ alpha 2‑Antiplasmin in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human (rh) Serpin F2 and less than 1% cross-reactivity with recombinant mouse (rm) Serpin C1, rmSerpin D1, rhSerpin A1, rmSerpin A5, and rhSerpin C1 is observed.
Serpin F2/ alpha 2‑Antiplasmin in Mouse Intestine.
Serpin F2/ alpha 2‑Antiplasmin was detected in perfusion fixed frozen sections of mouse intestine using 5 µg/mL Goat Anti-Mouse Serpin F2/ alpha 2‑Antiplasmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1239) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin F2/alpha 2-Antiplasmin
Serpin F2 is a member of the Serpin superfamily and the primary physiological inhibitor of the serine protease plasmin, which is responsible for the dissolution of fibrin clots (1, 2). In addition to plasmin, Serpin F2 is also an efficient inhibitor of trypsin and chymotrypsin (3). Liver and kidney are major sites of Serpin F2 production and other tissues such as muscle, intestine, central nervous system, and placenta also express its mRNA at a moderate level (3). The tissue expression pattern of Serpin F2 indicates that it is a key regulator of plasmin-mediated proteolysis in these tissues. Mouse Serpin F2 is synthesized as a 491 amino acid precursor with a 27 amino acid signal peptide. The secreted protein has a short propeptide (residues 28-39) and a mature chain (residues 40-491). For human Serpin F2, the presence of the propeptide did not affect its ability to inhibit plasmin but reduced its cross-linking ability to fibrin (4).
Menoud, P-A. et al. (1996) J. Clin. Invest. 97:2478.
Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
Potempa, J. et al. (1988) Science. 241:699.
Sumi, Y. et al. (1989) J. Biochem. 106:703.
Entrez Gene IDs:
5345 (Human); 18816 (Mouse)
AAPclade F (alpha-2 antiplasmin; alpha 2-Antiplasmin; Alpha-2-AP; Alpha-2-PI; Alpha-2-plasmin inhibitor; pigment epithelium derived factor), member 2; Serpin F2; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epitheliumderived factor), member 2
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