Detection of Mouse SLC39A4 by Western Blot.
Western blot shows lysates of Hepa 1‑6 mouse hepatoma cell line and D3 mouse embryonic stem cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse SLC39A4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7315) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SLC39A4 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of SLC39A4 in Hepa 1‑6 Mouse Cell Line by Flow Cytometry.
Hepa 1‑6 mouse hepatoma cell line was stained with Goat Anti-Mouse SLC39A4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7315, filled histogram) or control antibody (Catalog # AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
SLC39A4 was detected in immersion fixed frozen sections of adult mouse small intestine using Goat Anti-Mouse SLC39A4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7315) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to intestinal epithelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
SLC39A4 (Solute carrier family 39 member 4; also Zip-4) is a 75-90 kDa member of the ZIP transporter family of proteins. It is expressed by select cell types, including pancreatic beta -cells plus epithelium of the choroid plexus and intestine. SLC39A4 is part of a zinc-sensitive cell cycling system. When extracellular zinc levels are high, SLC39A4 is generally found internally associated with endosomes, and is unavailable at the cell membrane to transport zinc into the cell. Under low zinc conditions, SLC39A4 generally appears in the plasma membrane to ensure adequate zinc uptake in the face of limited zinc availability. Mouse SLC39A4 is a 660 amino acid (aa) six transmembrane (TM) glycoprotein. It contains a long N-terminal extracellular region (aa 1-337) followed by six TM segments (aa 338-651) and a short C-terminal nine aa extracellular tail (aa 652-660). There is one splice variant that shows a 16 aa substitution for aa 1-63. SLC39A4 undergoes cell-specific proteolytic processing in response to low zinc concentrations. Cleavage occurs in the N-terminal extracellular region to generate a soluble 35 kDa polypeptide and a 37 kDa six TM integral membrane protein. This does not appear to be an inactivating mechanism. Over aa 1-337, mouse SLC39A4 shares 84% and 59% aa sequence identity with rat and human SLC39A4, respectively.
Solute Carrier Family 39 Member 4
Entrez Gene IDs:
55630 (Human); 72027 (Mouse); 300051 (Rat)
acrodermatitis enteropathica, zinc-deficiency type; AEZ; FLJ20327; MGC74741; SLC39A4; solute carrier family 39 (zinc transporter), member 4; Solute carrier family 39 member 4; zinc transporter ZIP4; ZIP4; ZIP-4; ZIP4AEZ; Zrt- and Irt-like protein 4
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