Mouse SR-AI/MSR Antibody

Catalog # Availability Size / Price Qty
AF1797
AF1797-SP

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Product Details
Citations (11)
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Mouse SR-AI/MSR Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse SR-A1/MSR in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SR-AI/MSR
Trp79-Ser454
Accession # AAA39747
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse SR-AI/MSR (Catalog # 1797-MS)
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.5-2 µg/mL of this antibody will block 50% of the binding of 100 ng/mL of biotinylated AGE‑BSA to immobilized Recombinant Mouse SR-AI/MSR1 (Catalog # 1797-MS) coated at 5 µg/mL (100 µL/well). At 20 µg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SR-AI/MSR

The scavenger receptor (SR) family comprises a group of functionally defined membrane receptors that share the common ability to bind and internalize modified forms of Low Density Lipoproteins (mLDL) (1 - 3). Family members are classified alphabetically. The A class include four proteins: the three subtypes of SR-A (AI, AII, and AIII) that are generated by alternative splicing of the same gene, and a structurally similar protein named MARCO (4). All A class SRs are multidomain trimeric type II membrane proteins. SR-AI has an N-terminal cytoplasmic domain, a transmembrane domain, a spacer domain, an alpha -helical coiled coil, a collagen-like domain and a C-terminal cysteine-rich domain. SR-A is expressed by most tissue macrophages, dendritic cells and Kupffer cells. It is also highly expressed by microglia in neonatal as well as Alzheimer’ Disease brains. SR-AI binds a broad range of polyanionic ligands including modified proteins (e.g. Oxidized, acetylated or maleylated LDL, Advanced glycation end-product proteins), polyribonucleotides (polyguanosine and polyinosine), polysaccharides (dextran sulfate, fucoidan), phospholipids (phosphatidylserine), bacterial products (lipopolysaccharide and lipoteichoic acid) and selected chemical compounds (silica, crocidolite asbestos). The ligand-binding region has been localized to a positively charged region in the carboxyl end of the collagen-like domain. Based on its ligand binding characteristics, SR-AI is implicated in many physiological and pathophysiological functions. Studies using SR-A knockout mouse have also suggested roles of SR-A in atherogenesis, host defense and innate immunity, acquired immune responses, macrophage adhesion, and phagocytosis of apoptotic cells (1 - 3).

References
  1. Daugherty, A. et al. (2000) Curr. Opin. Cardiovasc. Pulm. Ren. Invest. Drugs 2:223.
  2. Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649.
  3. Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193.
  4. Elomaa, O. et al. (1995) Cell 80:603.
Long Name
Macrophage Scavenger Receptor Types I and II
Entrez Gene IDs
4481 (Human); 20288 (Mouse); 25073 (Rat)
Alternate Names
CD204 antigen; CD204; Macrophage acetylated LDL receptor I and II; macrophage scavenger receptor 1; macrophage scavenger receptor type III; MSR1; phSR1; phSR2; SCARA1; SCARA1macrophage scavenger receptor types I and II; Scavenger receptor class A member 1; scavenger receptor class A, member 1; SR-A; SRAI; SR-AI

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Citations for Mouse SR-AI/MSR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Advanced glycation end-products reduce lipopolysaccharide uptake by macrophages
    Authors: A Kitaura, T Nishinaka, S Hamasaki, OF Hatipoglu, H Wake, M Nishibori, S Mori, S Nakao, H Takahashi
    PLoS ONE, 2021;16(1):e0245957.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Scavenger receptor-A is a biomarker and effector of rheumatoid arthritis: A large-scale multicenter study
    Authors: F Hu, X Jiang, C Guo, Y Li, S Chen, W Zhang, Y Du, P Wang, X Zheng, X Fang, X Li, J Song, Y Xie, F Huang, J Xue, M Bai, Y Jia, X Liu, L Ren, X Zhang, J Guo, H Pan, Y Su, H Yi, H Ye, D Zuo, J Li, H Wu, Y Wang, R Li, L Liu, XY Wang, Z Li
    Nat Commun, 2020;11(1):1911.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. LncRNA kcnq1ot1 promotes lipid accumulation and accelerates atherosclerosis via functioning as a ceRNA through the miR-452-3p/HDAC3/ABCA1 axis
    Authors: XH Yu, WY Deng, JJ Chen, XD Xu, XX Liu, L Chen, MW Shi, QX Liu, M Tao, K Ren
    Cell Death & Disease, 2020;11(12):1043.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Effects of scavenger receptors-1 class A stimulation on macrophage morphology and highly modified advanced glycation end product-protein phagocytosis
    Authors: S Hamasaki, T Kobori, Y Yamazaki, A Kitaura, A Niwa, T Nishinaka, M Nishibori, S Mori, S Nakao, H Takahashi
    Sci Rep, 2018;8(1):5901.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Exercise training enhances in vivo clearance of endotoxin and attenuates inflammatory responses by potentiating Kupffer cell phagocytosis
    Authors: S Komine, K Akiyama, E Warabi, S Oh, K Kuga, K Ishige, S Togashi, T Yanagawa, J Shoda
    Sci Rep, 2017;7(1):11977.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Autophagy regulates phagocytosis by modulating the expression of scavenger receptors.
    Authors: Bonilla D, Bhattacharya A, Sha Y, Xu Y, Xiang Q, Kan A, Jagannath C, Komatsu M, Eissa N
    Immunity, 2013;39(3):537-47.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. The receptor of advanced glycation end products plays a central role in advanced oxidation protein products-induced podocyte apoptosis.
    Kidney Int, 2012;82(7):759-70.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  8. CD204 suppresses large heat shock protein-facilitated priming of tumor antigen gp100-specific T cells and chaperone vaccine activity against mouse melanoma.
    Authors: Qian J, Yi H, Guo C, Yu X, Zuo D, Chen X, Kane JM, Repasky EA, Subjeck JR, Wang XY
    J. Immunol., 2011;187(6):2905-14.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Advanced glycation end products induce production of reactive oxygen species via the activation of NADPH oxidase in murine hepatic stellate cells.
    Authors: Guimaraes EL, Empsen C, Geerts A, van Grunsven LA
    J. Hepatol., 2010;52(3):389-97.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Pattern recognition scavenger receptor SRA/CD204 down-regulates Toll-like receptor 4 signaling-dependent CD8 T-cell activation.
    Authors: Yi H, Yu X, Gao P, Wang Y, Baek SH, Chen X, Kim HL, Subjeck JR, Wang XY
    Blood, 2009;113(23):5819-28.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Cilostazol inhibits modified low-density lipoprotein uptake and foam cell formation in mouse peritoneal macrophages.
    Authors: Okutsu R, Yoshikawa T, Nagasawa M, Hirose Y, Takase H, Mitani K, Okada K, Miyakoda G, Yabuuchi Y
    Atherosclerosis, 2009;204(2):405-11.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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