Mouse TIMP-1 Antibody

(9 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TIMP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant rat TIMP-1 is observed, less than 5% cross-reactivity with recombinant human (rh) TIMP-1 is observed, and less than 1% cross-reactivity with rhTIMP-2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse TIMP-1
    Cys25-Arg205
    Accession # P12032
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse TIMP‑1 Western Blot Standard (Catalog # WBC022)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Mouse TIMP‑1 (Catalog # 980‑MT), see our available Western blot detection antibodies
  • Neutralization
    Measured by its ability to neutralize Recombinant Mouse TIMP-1 (0.07 µg/mL, Catalog # 980-MT) inhibition of Recombinant Mouse/Rat MMP‑2 (0.2 µg/mL, Catalog # 924-MP) cleavage of the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2 (10 µM, Catalog # ES001). The Neutralization Dose (ND50) is typically 1.3 µg/mL.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
TIMP-1 in Mouse Ovary. TIMP-1 was detected in perfusion fixed frozen sections of mouse ovary using Goat Anti-Mouse TIMP-1 Antigen Affinity-purified Polyclonal Antibody (red; Catalog # AF980) at 15 µg/mL overnight at 4 °C.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TIMP-1

Tissue inhibitors of metalloproteinases or TIMPs are a family of homologous proteins that regulate the activity of matrix metalloproteinases (MMPs) (1, 2). There are four known members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4 that have been found to exhibit multiple functions, including inhibition of active MMPs, pro‑MMP activation, cell growth promotion, matrix binding, inhibition of angiogenesis and the induction of apoptosis. Structurally, TIMPs have two domains, an N‑terminal domain and a C-terminal domain. Each domain consists of three disulfide-bonded loops. TIMP-1 is a glycoprotein produced by a wide range of cell types. Through its N-terminal domain, TIMP-1 inhibits active MMPs by forming a non-covalent binary complex with the MMP active site. The C-terminal domain of TIMP‑1 interacts with the C-terminal domain of pro‑MMP‑9, which may play a role in regulating pro‑MMP‑9 activation.

  • References:
    1. Murphy, G. and F. Willenbrock (1995) Methods Enzymol. 248:496.
    2. Brew, K. et al. (2000) Biochim. Biophys. Acta 1477:267.
  • Long Name:
    Tissue Inhibitors of Metalloproteinases 1
  • Entrez Gene IDs:
    7076 (Human); 21857 (Mouse); 116510 (Rat)
  • Alternate Names:
    CLGI; Collagenase inhibitor; collagenase inhibitor); EPATIMP-1; EPO; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; metalloproteinase inhibitor 1; TIMP metallopeptidase inhibitor 1; TIMP1; TIMP-1; TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity; Tissue inhibitor of metalloproteinases 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Applications
Sample Type
  1. Deletion of ADAM-9 in HGF/CDK4 mice impairs melanoma development and metastasis
    Authors: N Giebeler, A Schönefu beta, J Landsberg, T Tüting, C Mauch, P Zigrino
    Oncogene, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Fibroblast Activation Protein (FAP) Accelerates Collagen Degradation and Clearance from Lungs in Mice
    Authors: MH Fan, Q Zhu, HH Li, HJ Ra, S Majumdar, DL Gulick, JA Jerome, DH Madsen, M Christofid, DW Speicher, WW Bachovchin, C Feghali-Bo, E Puré
    J. Biol. Chem., 2016;291(15):8070-89.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. TAp73 is essential for germ cell adhesion and maturation in testis.
    Authors: Holembowski L, Kramer D, Riedel D, Sordella R, Nemajerova A, Dobbelstein M, Moll U
    J Cell Biol, 2014;204(7):1173-90.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  4. The MMP-9/TIMP-1 axis controls the status of differentiation and function of myelin-forming Schwann cells in nerve regeneration.
    Authors: Kim Y, Remacle AG, Chernov AV
    PLoS ONE, 2012;7(3):e33664.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  5. Differential vesicular distribution and trafficking of MMP-2, MMP-9, and their inhibitors in astrocytes.
    Authors: Sbai O, Ould-Yahoui A, Ferhat L, Gueye Y, Bernard A, Charrat E, Mehanna A, Risso JJ, Chauvin JP, Fenouillet E, Rivera S, Khrestchatisky M
    Glia, 2010;58(3):344-66.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  6. Amelioration of coxsackievirus B3-mediated myocarditis by inhibition of tissue inhibitors of matrix metalloproteinase-1.
    Authors: Crocker SJ, Frausto RF, Whitmire JK, Benning N, Milner R, Whitton JL
    Am. J. Pathol., 2007;171(6):1762-73.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  7. Upregulation of MMP-9/TIMP-1 enzymatic system in eosinophilic meningitis caused by Angiostrongylus cantonensis.
    Authors: Chen KM, Lee HH, Chou HL, Liu JY, Tsai BC, Lai SC
    Int J Exp Pathol, 2005;86(2):81-9.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  8. Tissue inhibitor of metalloproteinase-1 deficiency amplifies acute lung injury in bleomycin-exposed mice.
    Authors: Kim KH, Burkhart K, Chen P, Frevert CW, Randolph-Habecker J, Hackman RC, Soloway PD, Madtes DK
    Am. J. Respir. Cell Mol. Biol., 2005;33(3):271-9.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  9. Low-density lipoprotein receptor-related protein-1 mediates endocytic clearance of tissue inhibitor of metalloproteinases-1 and promotes its cytokine-like activities.
    Authors: Thevenard J, Verzeaux L, Devy J, Etique N, Jeanne A, Schneider C, Hachet C, Ferracci G, David M, Martiny L, Charpentier E, Khrestchatisky M, Rivera S, Dedieu S, Emonard H
    PLoS ONE, 0;9(7):e103839.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
Cell and Tissue Staining Kits
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Anti-Goat HRP-DAB Cell & Tissue Staining Kit

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Immunohistochemistry Reagents
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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

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Goat IgG HRP-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

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Goat IgG (H+L) APC-conjugated Antibody

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Goat IgG Horseradish Peroxidase-conjugated Antibody

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Donkey Anti-Goat IgG Antibody

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Donkey Anti-Goat IgG Biotinylated Antibody

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Goat IgG VisUCyte HRP Polymer

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Goat IgG (H+L) Fluorescein-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

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Rabbit Anti-Goat IgG Biotinylated Antibody

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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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Average Rating: 5 (Based on 1 review)

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Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedActivated mouse CD8 T cell
SpeciesMouse

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