Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Neutralization, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Immunocytochemistry, In vivo assay, Electron Microscopy

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TIMP-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TIMP-1
Cys25-Arg205
Accession # P12032

Specificity

Detects mouse TIMP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant rat TIMP-1 is observed, less than 5% cross-reactivity with recombinant human (rh) TIMP-1 is observed, and less than 1% cross-reactivity with rhTIMP-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse TIMP‑1 Antibody

TIMP-1 antibody in Mouse Ovary by Immunohistochemistry (IHC-Fr).

TIMP-1 in Mouse Ovary.

TIMP-1 was detected in perfusion fixed frozen sections of mouse ovary using Goat Anti-Mouse TIMP-1 Antigen Affinity-purified Polyclonal Antibody (red; Catalog # AF980) at 15 µg/mL overnight at 4 °C.
Detection of Mouse TIMP-1 by Western Blot

Detection of Mouse TIMP-1 by Western Blot

Conditioned media from G17-stimulated melanoma cells exhibit upregulation of MMP-2 and downregulation of TIMP-3 expression. Representative demonstration of the confirmation of proteomic data using Western blot analysis, with prosaposin serving as a benchmark for gastrin responsiveness (A). Mean and standard deviation (±SD) of the densitometric analysis results from three independent experiments (B). Statistical difference (* p < 0.05) between treated and untreated control groups (considered as 100%) are indicated as follows: prosaposin, TIMP-3 and TIMP-1 levels in G361 cells and MMP2 expressions in the SK-MEL-2 cell line. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/24/23/16851), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse TIMP‑1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse ovary

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse TIMP‑1 (Catalog # 980‑MT), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse TIMP‑1 Western Blot Standard (Catalog # WBC022)

Neutralization

Measured by its ability to neutralize Recombinant Mouse TIMP-1 (0.07 µg/mL, Catalog # 980-MT) inhibition of Recombinant Mouse/Rat MMP‑2 (0.2 µg/mL, Catalog # 924-MP) cleavage of the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2 (10 µM, Catalog # ES001). The Neutralization Dose (ND50) is typically 1.3 µg/mL.

Reviewed Applications

Read 3 reviews rated 4.3 using AF980 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TIMP-1

Tissue inhibitors of metalloproteinases or TIMPs are a family of homologous proteins that regulate the activity of matrix metalloproteinases (MMPs) (1, 2). There are four known members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4 that have been found to exhibit multiple functions, including inhibition of active MMPs, pro‑MMP activation, cell growth promotion, matrix binding, inhibition of angiogenesis and the induction of apoptosis. Structurally, TIMPs have two domains, an N‑terminal domain and a C-terminal domain. Each domain consists of three disulfide-bonded loops. TIMP-1 is a glycoprotein produced by a wide range of cell types. Through its N-terminal domain, TIMP-1 inhibits active MMPs by forming a non-covalent binary complex with the MMP active site. The C-terminal domain of TIMP‑1 interacts with the C-terminal domain of pro‑MMP‑9, which may play a role in regulating pro‑MMP‑9 activation.

References

  1. Murphy, G. and F. Willenbrock (1995) Methods Enzymol. 248:496.
  2. Brew, K. et al. (2000) Biochim. Biophys. Acta 1477:267.

Long Name

Tissue Inhibitors of Metalloproteinases 1

Alternate Names

TIMP1

Entrez Gene IDs

7076 (Human); 21857 (Mouse); 116510 (Rat)

Gene Symbol

TIMP1

UniProt

P12032

Additional TIMP-1 Products

Product Documents for Mouse TIMP‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse TIMP‑1 Antibody

For research use only

Citations for Mouse TIMP‑1 Antibody

Customer Reviews for Mouse TIMP‑1 Antibody (3)

4.3 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
67%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Mouse TIMP-1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Skeletal muscle
    Species: Mouse
    Verified Customer | Posted 01/25/2024
    Fresh frozen skeletal muscle tissue from wt mouse (11 months old)
    Mouse TIMP‑1 Antibody AF980
  • Mouse TIMP-1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: activated mouse CD8 T cell
    Species: Mouse
    Verified Customer | Posted 04/26/2017
  • Mouse TIMP-1 Antibody
    Name: Ruhul Choudhury
    Application: Immunohistochemistry-Paraffin
    Sample Tested: First trimester human decidua
    Species: Goat
    Verified Customer | Posted 01/20/2017
    20X magnification. First trimester human decidua express TIMP-1.
    sodium citrate antigen retrieval
    Mouse TIMP‑1 Antibody AF980

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Protocols

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