Mouse TLR4 Antibody (1203B)

Name: Mouse TLR4 Antibody (1203B)
Brand: R&D Systems
SKU: MAB27591
Price: 469 USD
Availability: InStock

R&D Systems | Catalog # MAB27591

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Citations (6)

Product Documents

Recombinant Monoclonal Antibody.

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry

Cited:

Flow Cytometry, Immunocytochemistry, Bioassay

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1203B

View all TLR4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TLR4
Asn26-Lys629
Accession # Q9QUK6

Specificity

Detects mouse TLR4 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mouse TLR4 Antibody (1203B)

Detection of TLR4 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rabbit Anti-Mouse TLR4 Monoclonal Antibody (Catalog # MAB27591, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110).

TLR4 in RAW 264.7 Mouse Cell Line.

TLR4 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Rabbit Anti-Mouse TLR4 Monoclonal Antibody (Catalog # MAB27591) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of TLR4 by Immunocytochemistry/ Immunofluorescence

(s)uPAR is a part of TLR4 interactome. (A) Primary peritoneal macrophages from uPAR−/− mice were stimulated with suPAR with or without LPS for 15 min. Then, cells were fixed and stained for TLR4 (Alexa 488, green) and uPAR (Alexa 647, red). DAPI was used as nuclear stain. Scale bar 10 μm. (B) Primary WT and uPAR−/− macrophages were stimulated with 50 ng/ml LPS and 1 μg/ml suPAR for 3 h. Data are presented as mean ± SD from two independent experiments performed in duplicates with pooled PM isolated from n=4 mice per group. *P < 0.05; **P < 0.01; ***P < 0.001. (C) Raw 264.7 cells were stimulated with 100 ng/ml LPS, fixed and stained as in A. Scale bar 12.5 μm. (D) Raw 264.7 cells were stimulated with 1 μg/ml biotin-LPS for 30 min, then cell lysis was performed. Protein complexes were precipitated using streptavidin magnetic beads and analyzed by western blotting using anti-murine uPAR antibody. One of 3 independent experiments with similar results is shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33362762), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse TLR4 Antibody (1203B)

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line

Immunocytochemistry

1-25 µg/mL
Sample: Immersion fixed RAW 264.7 mouse monocyte/macrophage cell line

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TLR4

TLR4 belongs to the IL-1 receptor/Toll-like receptor superfamily. It is expressed in placenta and peripheral blood leukocytes. TLR4 is the pattern recognition receptor for LPS.