Mouse TrkB Antibody

Catalog # Availability Size / Price Qty
AF1494
AF1494-SP
Detection  of Mouse TrkB by Western Blot.
3 Images
Product Details
Citations (13)
FAQs
Supplemental Products
Reviews (1)

Mouse TrkB Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TrkB in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant human (rh) TrkB is observed, and less than 2% cross-reactivity with rhTrkA, recombinant rat TrkA, and recombinant mouse TrkC is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TrkB
Cys32-His429
Accession # P15209
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.5-2 µg/mL of this antibody will block 50% of the binding of 5 ng/mL of biotinylated recombinant Human BDNF to immobilized Recombinant Mouse TrkB (Catalog # 1494-TB) coated at 2 µg/mL (100 µL/well). At 50 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Mouse TrkB by Western Blot. View Larger

Detection of Mouse TrkB by Western Blot. Western blot shows lysates of mouse brain (cerebellum) tissue and mouse brain (cortex) tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse TrkB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1494) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for TrkB at approximately 90-100 and 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry TrkB in Mouse Spinal Cord. View Larger

TrkB in Mouse Spinal Cord. TrkB was detected in perfusion fixed frozen sections of mouse spinal cord using Goat Anti-Mouse TrkB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1494) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to neuronal processes and cell bodies. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Simple Western Detection  of Mouse TrkB by Simple WesternTM. View Larger

Detection of Mouse TrkB by Simple WesternTM. Simple Western lane view shows lysates of mouse brain (cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for TrkB at approximately 154 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse TrkB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1494) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TrkB

The neurotrophins, including NGF, BDNF, NT-3, and NT-4/5, constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to and activating two unrelated receptor types: the p75 neurotrophin receptor (p75NTR) and the Trk family of receptor tyrosine kinases (1, 2). p75NTR is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF16. It binds all neurotrophins with low affinity to transduce cellular signaling pathways that synergize with or antagonize those activated by the Trk receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF and NT-3, TrkB binds BDNF, NT-3, and NT-4/5, and TrkC only binds NT-3 (1-2). All Trk family proteins share a conserved, complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain (3). Natural splice variants of the different Trks, lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described (4). At the protein sequence level, human and mouse TrkB show 94% amino acid sequence identity (5-6). The proteins also exhibit cross-species activity. The primary location of TrkB expression is in the central and peripheral nervous systems. Low level TrkB expression has also been observed in a wide variety of tissues outside the nervous system (6).

References
  1. Huang, E.J. and L.F. Reichardt (2003) Annu. Rev. Biochem. 72:609.
  2. Dechant, G. (2001) Cell Tissue Res. 305:229.
  3. Schneider, R. and M. Schweiger (1991) Oncogene 6:1807.
  4. Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
  5. Nakagawara A. et al. (1995) Genomics. 25:538.
  6. Klein, R. et al. (1989) EMBO J. 8:3701.
Long Name
Neurotrophic Tyrosine Kinase Receptor B
Entrez Gene IDs
4915 (Human); 18212 (Mouse); 25054 (Rat)
Alternate Names
BDNF/NT-3 growth factors receptor; EC 2.7.10; EC 2.7.10.1; GP145-TrkB; Neurotrophic tyrosine kinase receptor type 2; neurotrophic tyrosine kinase, receptor, type 2; NTRK2; TrkB tyrosine kinase; TrkB; trk-B; TRKBBDNF-tropomyosine receptor kinase B; tyrosine kinase receptor B

Product Datasheets

Citations for Mouse TrkB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells
    Authors: AJ Alshawaf, S Viventi, W Qiu, G D'Abaco, B Nayagam, M Erlichster, G Chana, I Everall, J Ivanusic, E Skafidas, M Dottori
    Sci Rep, 2018;8(1):603.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Characteristics of sensory neuronal groups in CGRP-cre-ER reporter mice: Comparison to Nav1.8-cre, TRPV1-cre and TRPV1-GFP mouse lines
    Authors: MJ Patil, AH Hovhannisy, AN Akopian
    PLoS ONE, 2018;13(6):e0198601.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Protection of nigral dopaminergic neurons by AAV1 transduction with Rheb(S16H) against neurotoxic inflammation in vivo
    Authors: S Kim, GJ Moon, YS Oh, J Park, WH Shin, JY Jeong, KS Choi, BK Jin, N Kholodilov, RE Burke, HJ Kim, CM Ha, SG Lee, SR Kim
    Exp. Mol. Med., 2018;50(2):e440.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  4. Genetic Tracing of Cav3.2 T-Type Calcium Channel Expression in the Peripheral Nervous System
    Authors: YA Bernal Sie, J Haseleu, A Kozlenkov, V Bégay, GR Lewin
    Front Mol Neurosci, 2017;10(0):70.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Development of a pharmacodynamic biomarker to measure target engagement from inhibition of the NGF-TrkA pathway
    Authors: EA Price, A Krasowska-, KK Nanda, SJ Stachel, DA Henze
    J. Neurosci. Methods, 2017;282(0):34-42.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: ELISA Capture
  6. Treadmill exercise suppressed stress-induced dendritic spine elimination in mouse barrel cortex and improved working memory via BDNF/TrkB pathway
    Authors: K Chen, L Zhang, M Tan, CS Lai, A Li, C Ren, KF So
    Transl Psychiatry, 2017;7(3):e1069.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  7. Pericytes of Multiple Organs Do Not Behave as Mesenchymal Stem Cells In�Vivo
    Authors: N Guimar?es-, P Cattaneo, Y Sun, T Moore-Morr, Y Gu, ND Dalton, E Rockenstei, E Masliah, KL Peterson, WB Stallcup, J Chen, SM Evans
    Cell Stem Cell, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - Frozen
  8. KCNQ Potassium Channels Modulate Sensitivity of Skin Down-hair (D-hair) Mechanoreceptors
    Authors: S Schütze, IJ Orozco, TJ Jentsch
    J. Biol. Chem, 2016;291(11):5566-75.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  9. Merkel Cell-Driven BDNF Signaling Specifies SAI Neuron Molecular and Electrophysiological Phenotypes
    Authors: EG Reed-Geagh, MC Wright, LA See, PC Adelman, KH Lee, HR Koerber, SM Maricich
    J Neurosci, 2016;36(15):4362-76.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - Frozen
  10. Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.
    Authors: Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P
    Nat Neurosci, 2015;18(1):145-53.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Protein tyrosine phosphatase receptor type O inhibits trigeminal axon growth and branching by repressing TrkB and Ret signaling.
    Authors: Gatto G, Dudanova I, Suetterlin P, Davies A, Drescher U, Bixby J, Klein R
    J Neurosci, 2013;33(12):5399-410.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  12. Sortilin associates with Trk receptors to enhance anterograde transport and neurotrophin signaling.
    Authors: Vaegter CB, Jansen P, Fjorback AW
    Nat. Neurosci., 2011;14(1):54-61.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  13. Embryonic sympathoblasts transiently express TrkB in vivo and proliferate in response to brain-derived neurotrophic factor in vitro.
    Authors: Straub JA, Sholler GL, Nishi R
    BMC Dev. Biol., 2007;7(0):10.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: IHC Frozen

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Mouse TrkB Antibody

Average Rating: 4 (Based on 1 Review)

5 Star
0%
4 Star
100%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Mouse TrkB Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Mouse TrkB Antibody
By Anonymous on 09/12/2016
Application: IHC Sample Tested: Brain (cortex) tissue,Adult brain (hippocampus) Species: Mouse