Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, Avian - Chicken, Primate, Primate - Callithrix jacchus (Common Marmoset), Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Blockade of Receptor-ligand Interaction, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Bioassay, ELISA Capture, ELISA Development (Capture), IHC fixed frozen, IHC-F

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TrkB Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TrkB
Cys32-His429
Accession # P15209

Specificity

Detects mouse TrkB in direct ELISAs. Detects human, mouse, and rat TrkB in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human/Mouse/Rat TrkB Antibody

Detection of Human, Mouse, and Rat TrkB antibody by Western Blot.

Detection of Human, Mouse, and Rat TrkB by Western Blot.

Western blot shows lysates of human brain (motor cortex) tissue, mouse brain (cortex) tissue, and rat brain (hippocampus) tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/Rat TrkB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1494) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for TrkB at approximately 95 kDa and 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse TrkB antibody by Western Blot.

Detection of Mouse TrkB by Western Blot.

Western blot shows lysates of mouse brain (cerebellum) tissue and mouse brain (cortex) tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat TrkB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1494) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for TrkB at approximately 90-100 and 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TrkB antibody in Mouse Spinal Cord by Immunohistochemistry (IHC-Fr).

TrkB in Mouse Spinal Cord.

TrkB was detected in perfusion fixed frozen sections of mouse spinal cord using Goat Anti-Human/Mouse/Rat TrkB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1494) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to neuronal processes and cell bodies. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Detection of Mouse TrkB antibody by Simple WesternTM.

Detection of Mouse TrkB by Simple WesternTM.

Simple Western lane view shows lysates of mouse brain (cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for TrkB at approximately 154 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat TrkB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1494) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of TrkB by Western Blot

Detection of TrkB by Western Blot

Expression profile of neurotrophic factors and receptors 7 days post surgery.Seven days post surgery, injured (R) and intact (L) sides of C5–C7 segments were collected separately for Western blot with anti- TrkB, p75, BDNF, NT-3 and GDNF antibodies in control (Ctrl) and Celsr3|Emx1 (Mut) mice, and beta -tubulin was used as a control protein (A). The expression level of each protein was normalized to beta -tubulin (B). Comparisons were summarized in C. In control mices, the expression of TrkB and BDNF was significantly decreased, but that of p75 was significantly increased, on injured sides (R) compared to intact sides (L). There was not significant difference in expression of neurotrophic factors and receptors between injured and intact sides in mutant mice. On both sides, the expression level of p75 was significantly lower in the mutant than in the control. On injured sides, the expression of TrkB and BDNF was significantly higher in the mutant than in the control although their expression was comparable on intact sides between two groups. “↓”, lower or decreased expression; “↑”, higher or increased expression; “_”, no significant difference; *, P<0.05; **, P<0.01; Ctrl-L, left side of control mice; Ctrl-R, right side of control mice; Mut-L, left side of mutant mice; Mut-R, right side of mutant mice. Six animals were used in each group and t-test was used for comparisons. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25003601), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse TrkB by Immunohistochemistry

Detection of Mouse TrkB by Immunohistochemistry

Normal developmental Trk expression dynamics in the trigeminal ganglion and maxillary nerve.(A–C) Representative images of fluorescent immunohistochemistry on serial horizontal sections showing TrkA (A, green), TrkB (B, green), or TrkC (C, green) with Isl1 (A–C, purple) in Control embryos at embryonic day 11 (E11). Arrows point to the maxillary nerve (MxV). (D and E) Representative maximum intensity projections of confocal Z-stacks through the Control maxillary process at E11.5 after whole-mount immunohistochemistry to detect Tubb3 (D, white) and TrkA (E, white), followed by tissue clearing. (F–K) Representative images of fluorescent immunohistochemistry on serial horizontal sections showing TrkA (F and I, green), TrkB (G and J, green), or TrkC (H and K, green) in Control embryos at E11.5 (F–H) and E12.5 (I–K). Scale bars: 50 µm (A), applies to (B and C); 100 µm (D), applies to (E); 100 µm (F), applies to (G and K). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35713404), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse TrkB by Immunohistochemistry

Detection of Mouse TrkB by Immunohistochemistry

Normal developmental Trk expression dynamics in the trigeminal ganglion and maxillary nerve.(A–C) Representative images of fluorescent immunohistochemistry on serial horizontal sections showing TrkA (A, green), TrkB (B, green), or TrkC (C, green) with Isl1 (A–C, purple) in Control embryos at embryonic day 11 (E11). Arrows point to the maxillary nerve (MxV). (D and E) Representative maximum intensity projections of confocal Z-stacks through the Control maxillary process at E11.5 after whole-mount immunohistochemistry to detect Tubb3 (D, white) and TrkA (E, white), followed by tissue clearing. (F–K) Representative images of fluorescent immunohistochemistry on serial horizontal sections showing TrkA (F and I, green), TrkB (G and J, green), or TrkC (H and K, green) in Control embryos at E11.5 (F–H) and E12.5 (I–K). Scale bars: 50 µm (A), applies to (B and C); 100 µm (D), applies to (E); 100 µm (F), applies to (G and K). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35713404), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse TrkB by Immunohistochemistry

Detection of Mouse TrkB by Immunohistochemistry

Normal developmental Trk expression dynamics in the trigeminal ganglion and maxillary nerve.(A–C) Representative images of fluorescent immunohistochemistry on serial horizontal sections showing TrkA (A, green), TrkB (B, green), or TrkC (C, green) with Isl1 (A–C, purple) in Control embryos at embryonic day 11 (E11). Arrows point to the maxillary nerve (MxV). (D and E) Representative maximum intensity projections of confocal Z-stacks through the Control maxillary process at E11.5 after whole-mount immunohistochemistry to detect Tubb3 (D, white) and TrkA (E, white), followed by tissue clearing. (F–K) Representative images of fluorescent immunohistochemistry on serial horizontal sections showing TrkA (F and I, green), TrkB (G and J, green), or TrkC (H and K, green) in Control embryos at E11.5 (F–H) and E12.5 (I–K). Scale bars: 50 µm (A), applies to (B and C); 100 µm (D), applies to (E); 100 µm (F), applies to (G and K). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35713404), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse TrkB by Immunohistochemistry

Detection of Mouse TrkB by Immunohistochemistry

Normal developmental Trk expression dynamics in the trigeminal ganglion and maxillary nerve.(A–C) Representative images of fluorescent immunohistochemistry on serial horizontal sections showing TrkA (A, green), TrkB (B, green), or TrkC (C, green) with Isl1 (A–C, purple) in Control embryos at embryonic day 11 (E11). Arrows point to the maxillary nerve (MxV). (D and E) Representative maximum intensity projections of confocal Z-stacks through the Control maxillary process at E11.5 after whole-mount immunohistochemistry to detect Tubb3 (D, white) and TrkA (E, white), followed by tissue clearing. (F–K) Representative images of fluorescent immunohistochemistry on serial horizontal sections showing TrkA (F and I, green), TrkB (G and J, green), or TrkC (H and K, green) in Control embryos at E11.5 (F–H) and E12.5 (I–K). Scale bars: 50 µm (A), applies to (B and C); 100 µm (D), applies to (E); 100 µm (F), applies to (G and K). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35713404), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of TrkB by Western Blot

Detection of TrkB by Western Blot

TrkB protein expression in DRG is decreased following SCI. A, Histogram shows that TrkB expression in DRGs are significantly decreased 5–7 d after SCI (open circles) compared with uninjured condition (black-filled circles; *p = 0.0469). B, Representative Western blot images are shown for TrkB 95 expression in the thoracic DRGs from uninjured and SCI animals (TrkB145 was not observed). C, Double immunofluorescence demonstrates TrkB expression (red fluorescence) in NeuN-positive neuron (green fluorescence; merged, yellow fluorescence). Scale bars represent 50 μm. Differential pattern of colocalization (indicated by arrowheads) of TrkB and NeuN shows a potentially altered expression of cell-surface TrkB in uninjured (left) versus SCI (right) small-diameter DRG neurons. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39753357), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of TrkB by Western Blot

Detection of TrkB by Western Blot

TrkB protein expression in DRG is decreased following SCI. A, Histogram shows that TrkB expression in DRGs are significantly decreased 5–7 d after SCI (open circles) compared with uninjured condition (black-filled circles; *p = 0.0469). B, Representative Western blot images are shown for TrkB 95 expression in the thoracic DRGs from uninjured and SCI animals (TrkB145 was not observed). C, Double immunofluorescence demonstrates TrkB expression (red fluorescence) in NeuN-positive neuron (green fluorescence; merged, yellow fluorescence). Scale bars represent 50 μm. Differential pattern of colocalization (indicated by arrowheads) of TrkB and NeuN shows a potentially altered expression of cell-surface TrkB in uninjured (left) versus SCI (right) small-diameter DRG neurons. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39753357), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of TrkB by Immunocytochemistry/ Immunofluorescence

Detection of TrkB by Immunocytochemistry/ Immunofluorescence

TrkB protein expression in DRG is decreased following SCI. A, Histogram shows that TrkB expression in DRGs are significantly decreased 5–7 d after SCI (open circles) compared with uninjured condition (black-filled circles; *p = 0.0469). B, Representative Western blot images are shown for TrkB 95 expression in the thoracic DRGs from uninjured and SCI animals (TrkB145 was not observed). C, Double immunofluorescence demonstrates TrkB expression (red fluorescence) in NeuN-positive neuron (green fluorescence; merged, yellow fluorescence). Scale bars represent 50 μm. Differential pattern of colocalization (indicated by arrowheads) of TrkB and NeuN shows a potentially altered expression of cell-surface TrkB in uninjured (left) versus SCI (right) small-diameter DRG neurons. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39753357), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat TrkB Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.5-2 µg/mL of this antibody will block 50% of the binding of 5 ng/mL of biotinylated recombinant Human BDNF to immobilized Recombinant Mouse TrkB (Catalog # 1494-TB) coated at 2 µg/mL (100 µL/well). At 50 μg/mL, this antibody will block >90% of the binding.

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain and spinal cord

Simple Western

10 µg/mL
Sample:   Mouse brain (cortex) tissue

Western Blot

0.1-0.5 µg/mL
Sample: Human brain (motor cortex) tissue, Mouse brain (cortex) tissue, Rat brain (hippocampus) tissue, and Mouse brain (cerebellum) tissue

Reviewed Applications

Read 17 reviews rated 4.6 using AF1494 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TrkB

The neurotrophins, including NGF, BDNF, NT-3, and NT-4/5, constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to and activating two unrelated receptor types: the p75 neurotrophin receptor (p75NTR) and the Trk family of receptor tyrosine kinases (1, 2). p75NTR is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF16. It binds all neurotrophins with low affinity to transduce cellular signaling pathways that synergize with or antagonize those activated by the Trk receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF and NT-3, TrkB binds BDNF, NT-3, and NT-4/5, and TrkC only binds NT-3 (1-2). All Trk family proteins share a conserved, complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain (3). Natural splice variants of the different Trks, lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described (4). At the protein sequence level, human and mouse TrkB show 94% amino acid sequence identity (5-6). The proteins also exhibit cross-species activity. The primary location of TrkB expression is in the central and peripheral nervous systems. Low level TrkB expression has also been observed in a wide variety of tissues outside the nervous system (6).

References

  1. Huang, E.J. and L.F. Reichardt (2003) Annu. Rev. Biochem. 72:609.
  2. Dechant, G. (2001) Cell Tissue Res. 305:229.
  3. Schneider, R. and M. Schweiger (1991) Oncogene 6:1807.
  4. Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
  5. Nakagawara A. et al. (1995) Genomics. 25:538.
  6. Klein, R. et al. (1989) EMBO J. 8:3701.

Long Name

Neurotrophic Tyrosine Kinase Receptor B

Alternate Names

NTRK2

Entrez Gene IDs

4915 (Human); 18212 (Mouse); 25054 (Rat)

Gene Symbol

NTRK2

UniProt

P15209

Additional TrkB Products

Product Documents for Human/Mouse/Rat TrkB Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat TrkB Antibody

For research use only

Citations for Human/Mouse/Rat TrkB Antibody

Customer Reviews for Human/Mouse/Rat TrkB Antibody (17)

4.6 out of 5
17 Customer Ratings
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Showing  1 - 5 of 17 reviews Showing All
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  • Human/Mouse/Rat TrkB Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Hippocampal neurons
    Species: Mouse
    Verified Customer | Posted 07/15/2021
    Human/Mouse/Rat TrkB Antibody AF1494
  • Human/Mouse/Rat TrkB Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Brain (hypothalamus) tissue
    Species: Mouse
    Verified Customer | Posted 05/03/2021
    Human/Mouse/Rat TrkB Antibody AF1494
  • Human/Mouse/Rat TrkB Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: E12 DRG neurons
    Species: Mouse
    Verified Customer | Posted 11/22/2019
    E12 mouse DRG neurons grown overnight with 10ng/ml NGF, then fixed in 4% PFA, blocked for 1 h with 5% BSA, and incubated overnight in 1% BSA containing AF1494 (1:300). A fluorophore-conjugated secondary antibody was used to visualise reactivity.
    Human/Mouse/Rat TrkB Antibody AF1494
  • Mouse TrkB Antibody
    Name: Emily Thompson
    Application: Western Blot
    Sample Tested: Brain (cortex) tissue
    Species: Mouse
    Verified Customer | Posted 04/04/2019
    Human/Mouse/Rat TrkB Antibody AF1494
  • Mouse TrkB Antibody
    Name: Juan Nacher
    Application: Immunohistochemistry
    Sample Tested: Brain (cortex) tissue and Adult brain (hippocampus)
    Species: Mouse
    Verified Customer | Posted 09/12/2016
    Human/Mouse/Rat TrkB Antibody AF1494
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23516305
    Species: Mouse
    Verified Customer | Posted 01/05/2015
  • Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: See PMID 23224557
    Species: Mouse
    Verified Customer | Posted 01/05/2015
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 23516305
    Species: Mouse
    Verified Customer | Posted 01/05/2015
  • Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: Cultured hippocampal neurons
    Species: Mouse
    Verified Customer | Posted 12/19/2014
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Cultured hippocampal neurons
    Species: Mouse
    Verified Customer | Posted 12/19/2014
  • Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: TrkB from hippocampal neurons
    Species: Mouse
    Verified Customer | Posted 12/19/2014
  • Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Hippocampal cryosections
    Species: Mouse
    Verified Customer | Posted 12/19/2014
  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Hippocampal neurons
    Species: Mouse
    Verified Customer | Posted 12/19/2014
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Hippocampus tissues
    Species: Mouse
    Verified Customer | Posted 12/19/2014
    WB analysis of TrkB in mouse synaptosomal preparations of mouse hippocampus
    Human/Mouse/Rat TrkB Antibody AF1494
  • Human/Mouse/Rat TrkB Antibody
    Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: Mouse hippocampal neurons
    Species: Mouse
    Verified Customer | Posted 12/16/2014
  • Human/Mouse/Rat TrkB Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: mouse hippocampus
    Species: Other
    Verified Customer | Posted 12/16/2014
  • Human/Mouse/Rat TrkB Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: mouse hippocampus
    Species: Other
    Verified Customer | Posted 12/16/2014

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