Mouse TrkB Antibody AF1494: R&D Systems

Mouse TrkB Antibody

(7 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TrkB in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant human (rh) TrkB is observed, and less than 2% cross-reactivity with rhTrkA, recombinant rat TrkA, and recombinant mouse TrkC is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse TrkB
    Cys32-His429
    Accession # P15209
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 0.5-2 µg/mL of this antibody will block 50% of the binding of 5 ng/mL of biotinylated recombinant Human BDNF to immobilized Recombinant Mouse TrkB (Catalog # 1494-TB) coated at 2 µg/mL (100 µL/well). At 50 μg/mL, this antibody will block >90% of the binding.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse TrkB by Western Blot. Western blot shows lysates of mouse brain (cerebellum) tissue and mouse brain (cortex) tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse TrkB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1494) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for TrkB at approximately 90-100 and 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
TrkB in Mouse Spinal Cord. TrkB was detected in perfusion fixed frozen sections of mouse spinal cord using Goat Anti-Mouse TrkB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1494) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to neuronal processes and cell bodies. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Detection of Mouse TrkB by Simple WesternTM. Simple Western lane view shows lysates of mouse brain (cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for TrkB at approximately 154 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse TrkB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1494) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TrkB

The neurotrophins, including NGF, BDNF, NT-3, and NT-4/5, constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to and activating two unrelated receptor types: the p75 neurotrophin receptor (p75NTR) and the Trk family of receptor tyrosine kinases (1, 2). p75NTR is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF16. It binds all neurotrophins with low affinity to transduce cellular signaling pathways that synergize with or antagonize those activated by the Trk receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF and NT-3, TrkB binds BDNF, NT-3, and NT-4/5, and TrkC only binds NT-3 (1-2). All Trk family proteins share a conserved, complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain (3). Natural splice variants of the different Trks, lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described (4). At the protein sequence level, human and mouse TrkB show 94% amino acid sequence identity (5-6). The proteins also exhibit cross-species activity. The primary location of TrkB expression is in the central and peripheral nervous systems. Low level TrkB expression has also been observed in a wide variety of tissues outside the nervous system (6).

  • References:
    1. Huang, E.J. and L.F. Reichardt (2003) Annu. Rev. Biochem. 72:609.
    2. Dechant, G. (2001) Cell Tissue Res. 305:229.
    3. Schneider, R. and M. Schweiger (1991) Oncogene 6:1807.
    4. Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
    5. Nakagawara A. et al. (1995) Genomics. 25:538.
    6. Klein, R. et al. (1989) EMBO J. 8:3701.
  • Long Name:
    Neurotrophic Tyrosine Kinase Receptor B
  • Entrez Gene IDs:
    4915 (Human); 18212 (Mouse); 25054 (Rat)
  • Alternate Names:
    BDNF/NT-3 growth factors receptor; EC 2.7.10; EC 2.7.10.1; GP145-TrkB; Neurotrophic tyrosine kinase receptor type 2; neurotrophic tyrosine kinase, receptor, type 2; NTRK2; TrkB tyrosine kinase; TrkB; trk-B; TRKBBDNF-tropomyosine receptor kinase B; tyrosine kinase receptor B
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Pericytes of Multiple Organs Do Not Behave as Mesenchymal Stem Cells In�Vivo
    Authors: N Guimar?es-, P Cattaneo, Y Sun, T Moore-Morr, Y Gu, ND Dalton, E Rockenstei, E Masliah, KL Peterson, WB Stallcup, J Chen, SM Evans
    Cell Stem Cell, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Frozen
  2. KCNQ Potassium Channels Modulate Sensitivity of Skin Down-hair (D-hair) Mechanoreceptors
    Authors: S Schütze, IJ Orozco, TJ Jentsch
    J. Biol. Chem, 2016;291(11):5566-75.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  3. Merkel Cell-Driven BDNF Signaling Specifies SAI Neuron Molecular and Electrophysiological Phenotypes
    Authors: EG Reed-Geagh, MC Wright, LA See, PC Adelman, KH Lee, HR Koerber, SM Maricich
    J Neurosci, 2016;36(15):4362-76.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Frozen
  4. Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.
    Authors: Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P
    Nat Neurosci, 2015;18(1):145-53.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  5. Protein tyrosine phosphatase receptor type O inhibits trigeminal axon growth and branching by repressing TrkB and Ret signaling.
    Authors: Gatto G, Dudanova I, Suetterlin P, Davies A, Drescher U, Bixby J, Klein R
    J Neurosci, 2013;33(12):5399-410.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  6. Sortilin associates with Trk receptors to enhance anterograde transport and neurotrophin signaling.
    Authors: Vaegter CB, Jansen P, Fjorback AW
    Nat. Neurosci., 2011;14(1):54-61.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  7. Embryonic sympathoblasts transiently express TrkB in vivo and proliferate in response to brain-derived neurotrophic factor in vitro.
    Authors: Straub JA, Sholler GL, Nishi R
    BMC Dev. Biol., 2007;7(0):10.
    Species: Chicken
    Sample Type: Whole Tissue
    Application: IHC Frozen
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 application: Immunohistochemistry.

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Immunohistochemistry Mouse TrkB Antibody AF1494
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 IHC Mouse Anonymous 09/12/2016
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Immunohistochemistry Mouse TrkB Antibody AF1494
Immunohistochemistry: Mouse TrkB Antibody [AF1494]

Summary

ApplicationImmunohistochemistry
Sample TestedBrain (cortex) tissue,Adult brain (hippocampus)
SpeciesMouse

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