Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Trypsin 3/PRSS3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Trypsin 3
Phe16-Asn246
Accession # NP_035775

Specificity

Detects mouse Trypsin 3 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) Trypsin 1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Trypsin 3/PRSS3 Antibody

Trypsin 3/PRSS3 antibody in Mouse Pancreas by Immunohistochemistry (IHC-Fr).

Trypsin 3/PRSS3 in Mouse Pancreas.

Trypsin 3/PRSS3 was detected in perfusion fixed frozen sections of mouse pancreas using 5 µg/mL Goat Anti-Mouse Trypsin 3/PRSS3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3565) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Trypsin 3/PRSS3 antibody in Mouse Pancreas by Immunohistochemistry (IHC-Fr).

Trypsin 3/PRSS3 in Mouse Pancreas.

Trypsin 3/PRSS3 was detected in perfusion fixed frozen sections of mouse pancreas using Mouse Trypsin 3/PRSS3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3565) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse Trypsin 3/PRSS3 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse pancreas

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Trypsin 3/PRSS3 (Catalog # 3565-SE), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Trypsin 3/PRSS3 (Catalog # 3565-SE)

Reviewed Applications

Read 1 review rated 5 using AF3565 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Trypsin 3/PRSS3

Mouse Trypsin 3, encoded by the PRSS3 gene, is also known as mesotrypsin. It consists of a signal peptide (residues 1-15), a pro region (residues 16-23), and a mature chain (residues 24-246) (1). The purified recombinant mouse Trypsin 3 corresponds to the pro form, which can be activated by enterokinase.

References

  1. Halfon, S. et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 1483, Academic Press, San Diego.

Alternate Names

Mesotrypsin, MTG, PRSS3, PRSS4, Trypsin 4, Trypsinogen 15

Entrez Gene IDs

5646 (Human); 22073 (Mouse); 362347 (Rat)

Gene Symbol

PRSS3

UniProt

NP_035775

Additional Trypsin 3/PRSS3 Products

Product Documents for Mouse Trypsin 3/PRSS3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Trypsin 3/PRSS3 Antibody

For research use only

Related Research Areas

Serine Proteases and Regulators

Citations for Mouse Trypsin 3/PRSS3 Antibody

Customer Reviews for Mouse Trypsin 3/PRSS3 Antibody (1)

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  • Mouse Trypsin 3/PRSS3 Antibody
    Name: Gonzalo Gonzalez
    Application: Western Blot
    Sample Tested: Recombinant protein and mouse feces
    Species: Mouse
    Verified Customer | Posted 05/02/2017
    1:1000 dilution in 1% milk 3 hours incubation @ RT

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Protocols

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