Mouse Trypsin 3/PRSS3 Antibody

Catalog # Availability Size / Price Qty
AF3565
AF3565-SP
Mouse Trypsin 3/PRSS3 Antibody in Data
2 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Mouse Trypsin 3/PRSS3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Trypsin 3 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) Trypsin 1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Trypsin 3
Phe16-Asn246
Accession # NP_035775
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Trypsin 3/PRSS3 (Catalog # 3565-SE)
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Trypsin 3/PRSS3 (Catalog # 3565-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry Trypsin 3/PRSS3 antibody in Mouse Pancreas by Immunohistochemistry (IHC-Fr). View Larger

Trypsin 3/PRSS3 in Mouse Pancreas. Trypsin 3/PRSS3 was detected in perfusion fixed frozen sections of mouse pancreas using 5 µg/mL Goat Anti-Mouse Trypsin 3/PRSS3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3565) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunohistochemistry Trypsin 3/PRSS3 antibody in Mouse Pancreas by Immunohistochemistry (IHC-Fr). View Larger

Trypsin 3/PRSS3 in Mouse Pancreas. Trypsin 3/PRSS3 was detected in perfusion fixed frozen sections of mouse pancreas using Mouse Trypsin 3/PRSS3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3565) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Trypsin 3/PRSS3

Mouse Trypsin 3, encoded by the PRSS3 gene, is also known as mesotrypsin. It consists of a signal peptide (residues 1-15), a pro region (residues 16-23), and a mature chain (residues 24-246) (1). The purified recombinant mouse Trypsin 3 corresponds to the pro form, which can be activated by enterokinase.

References
  1. Halfon, S. et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 1483, Academic Press, San Diego.
Entrez Gene IDs
5646 (Human); 22073 (Mouse); 362347 (Rat)
Alternate Names
Brain trypsinogen; EC 3.4.21; EC 3.4.21.4; Mesotrypsin; mesotrypsinogen; MTG; pancreatic trypsinogen III; protease, serine, 3 (mesotrypsin); protease, serine, 3; protease, serine, 4 (trypsin 4, brain); PRSS3; PRSS4; Serine protease 3; Serine protease 4; T9; TRY3MTG; TRY4mesotrypsin; Trypsin 3; Trypsin 4; Trypsin III; Trypsin IV; trypsin-3; Trypsinogen 15; trypsinogen 4; trypsinogen 5; trypsinogen IV

Product Datasheets

Citation for Mouse Trypsin 3/PRSS3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Heterogeneous Effects of Calorie Content and Nutritional Components Underlie Dietary Influence on Pancreatic Cancer Susceptibility
    Authors: J Dooley, V Lagou, J Goveia, A Ulrich, K Rohlenova, N Heirman, T Karakach, Y Lampi, S Khan, J Wang, T Dresselaer, U Himmelreic, MJ Gunter, I Prokopenko, P Carmeliet, A Liston
    Cell Rep, 2020;32(2):107880.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Mouse Trypsin 3/PRSS3 Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Mouse Trypsin 3/PRSS3 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Mouse Trypsin 3/PRSS3 Antibody
By Anonymous on 05/02/2017
Application: WB Sample Tested: mouse feces,Recombinant protein Species: Mouse

1:1000 dilution in 1% milk
3 hours incubation @ RT