Mouse Wnt-10b Antibody

R&D Systems | Catalog # AF2110

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Wnt-10b Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Wnt-10b peptide
Leu42-Glu82, Arg263-Arg332
Accession # P48614

Specificity

Detects mouse Wnt-10b in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant mouse (rm) Wnt-3a, rmWnt-4, rmWnt-5a, and rmWnt-8a is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Wnt-10b Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Wnt‑10b

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Wnt-10b

Wnt-10b (also known as Wnt-12) is a 45 kDa glycoprotein involved in fetal limb bud development and adult hematopoiesis. Mouse Wnt-10b exists as two isoforms: a 361 amino acid (aa) long form and a 265 aa short form that lacks a 95 aa internal sequence. Wnt-10b is less than 60% aa identical to other Wnts, but mouse to human, Wnt-10b shows almost complete aa sequence identity at > 97%.

Long Name

Wingless-type MMTV Integration Site Family, Member 10B

Alternate Names

Wnt-12, Wnt10b

Entrez Gene IDs

7480 (Human); 22410 (Mouse)

Gene Symbol

WNT10B

UniProt

P48614

Additional Wnt-10b Products

Product Documents for Mouse Wnt-10b Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Wnt-10b Antibody

For research use only

Related Research Areas

Wnt Ligands (Wnts)

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